A Paper Chromatographic Separation of Gibberellic Acid and Gibberellin A.

Abstract

HILTON ET natedl acetic acid was more inhibitory than TCA and was more readily antagonized by ,B-alanine.The inability of high concentrations of ,B-alanine completely to reverse the inhibitions produced by this class of compounds suggest that physical or chemical processes other than the fl-alanine pantothenic acid reaction(s) may be involved in the mechanisms of action. Nevertheless, the utilization of fl-alanine appears to be the most sensitive metabolic process involved under the experimental conditions employed; the enzymes involved in pantothenic acid synthesis should be investigated as a possible important site of action of these herbicidal compounds. Further investigations on this mechanism of action and its relative importance in species selectivity are in progress and will be the subject of future reports from this laboratory. SUMMARY Inhibition of yeast growth by chloro-substituted isobutyric, propionic, and acetic acids has been observed. An exogenous supply of 8-alanine has been shown to produce varying degrees of protection against these inhibitions.The results obtained suggest that these herbicides interfere with synthesis of pantothenic acid, presumablv by competition with ,p-alanine. LITERATURE CITED 1. KINGC, T. E. and CHELDELIN, V'. H. Pantothenic acid studies. IV. Propionic acid and 8-alanine utilization. Jour. Biol. Chem.

The Preparation of 14C-Labelled Gibberellic Acid

“…Spots of GA were detected either by their blue-green fluorescence under ultraviolet (u.v.) radiation after treatment with a mixture of ethanol + sulphuric acid (Curtis & Cross, 1954;Radley, 1956), or, along with other organic substances, by dipping in 0.4% KMnO, and washing in tap water (Bird & Pugh, 1958). On other occasions spots or bands of GA were eluted in 'elution tubes' (Canny, 1960).…”

Growth-Regulator Residues, Estimation of Gibberellic Acid Residue in Rhubarb

“…Exclusive of bioassay procedures, they can be classified as chromatographic methods, which include paper (2,73). thin layer (6,70,14).…”

Microbiological Production of Gibberellic Acid in Glucose Media1

“…Gibberellic acid was also identified by chromatography on Schleicher and Schuell no. 598 paper according to the method of Bird and Pugh (1958).…”