A paper microfluidics-based fluorescent lateral flow immunoassay for point-of-care diagnostics of non-communicable diseases

Natarajan, Satheesh; Su, Fengmei; Joseph, Jayaraj; Shah, Malay Ilesh; Huang, Youju

doi:10.1039/c9an01382b

Cited by 24 publications

(12 citation statements)

References 52 publications

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“…Sandwich type assays for cTnI have been implemented in LFA while using detection strategies that rely on fluorophores [24], quantum dots [28], gold nanoparticles [29], or Raman tags [30], to name a few. On all of these systems, as in most LFA devices, NC is used in the analytical strip.…”

Section: Resultsmentioning

confidence: 99%

“…We further study the effect of layering CNF on the test line regions of the cellulose strip in the signals generated ( Figure 1d). As a model system, we consider the quantitative detection of cardiac troponin I (cTnI), a biomarker of myocardial cell damage, by a fluorescent LFA [24]. The system relies on the recognition of the target cTnI molecule by fluorescently labelled anti-cTnI detection antibodies and subsequent capture of the complex at the test line by an anti-cTnI capturing antibody (Figure 1b,c).…”

Section: Introductionmentioning

confidence: 99%

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A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I

2021

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This paper presents a lateral flow assay (LFA) for the quantitative, fluorescence-based detection of the cardiac biomarker troponin I (cTnI) that features an analytical strip made of cellulose filter paper. The results show that the wicking and test time are comparable to those obtained with conventional nitrocellulose (NC)-based LFAs. Further, the cellulose paper provides an excellent background with no auto-fluorescence that is very adequate in detecting fluorescent lines. While fluorescence that was generated with cellulose strips was lower when compared to that generated in NC strips, signals could be improved by layering carbon nanofibers (CNF) on the cellulose. A nonlinear behavior of the concentration–response relationship was observed for the LFA architectures with NC, cellulose, and cellulose-CNF in the 0 to 200 ng/mL cTnI concentration range. The measurements were consistent and characterized by coefficients of variation lower than 2.5%. Detection and quantitation limits that were in the range 1.28–1.40 ng/mL and 2.10–2.75 ng/mL were obtained for LFA with cellulose and cellulose CNF strips that are equivalent to the limits obtained with the standard NC LFA. Overall, we showed that commercially available filter paper can be used in the analytical strip of LFA.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I

2021

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“…The comparison of the developed aptamer-based paper strip with previously reported assays for detection of cTnI is shown in Table S1 in the Supplementary Material . Compared with other paper-based assays, 16 , 25 , 26 the developed paper strip used an aptamer, which is more thermally stable, has better tolerance to wide range of pH, and is a lower cost recognition element (

times lower cost compared to the cost of cTnl antibodies purchased online). 34 , 35 In addition, using a portable reader, it showed good sensitivity close to the lower end of physiological range and required a shorter detection time than the other assays based on aptamers.…”

Section: Resultsmentioning

confidence: 99%

“…Recently, various POC testing systems for cTnI have been developed; 14 – 29 however, these systems have limitations in cost effectiveness (especially in the cost of the disposable cartridges), poor sensitivity, or limited stability of the recognition element. Paper-based sensing systems have demonstrated potential as a POC platform to implement assays due to the merits of paper (e.g., abundance, low cost, and ease of disposability).…”

Section: Introductionmentioning

confidence: 99%

“… 17 – 21 Paper-based SERS assays, in particular, have been developed for sensitive detection of cTnI. 15 , 16 , 22 , 23 However, these assays use antibodies as the recognition element, 15 , 17 – 23 which has limitations due to selection difficulties, high costs of production, and stability issues. 33 Aptamers, on the other hand, can be used as the recognition element for low-cost POC testing due to their nontoxicity, thermal stability, tolerance to a range of pH levels, and long shelf life.…”

Section: Introductionmentioning

confidence: 99%

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Aptamer-based surface-enhanced resonance Raman scattering assay on a paper fluidic platform for detection of cardiac troponin I

Holderby

Coté

2020

J. Biomed. Opt.

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Significance: Cardiac troponin I (cTnI) is a primary biomarker for diagnosis of myocardial infarction (MI). In contrast to central laboratory tests for cTnI, point-of-care (POC) testing has the advantage of providing results when the patient is first encountered, which helps high-risk patients to be treated more rapidly and low-risk patients to be released in a timely fashion. A paper fluidic platform is good for POC testing because the paper is abundant, low cost, and disposable. However, current cTnI assays on paper platforms use antibodies as the recognition element, which has limitations due to the high cost of production and antibody stability issues at the POC. Aim: To develop an aptamer-based assay on a paper strip using surface-enhanced resonance Raman spectroscopy (SERRS) for detection of cTnI in the clinically relevant range at the POC. Approach: Gold nanoparticles (AuNPs) were functionalized with a Raman reporter molecule, malachite green isothiocyanate. The functionalized AuNPs were encapsulated in a silica shell and provided a SERRS signal using a handheld Raman system with a 638-nm excitation wavelength. A primary aptamer and a secondary aptamer of cTnI were used in a sandwich assay format to bind the cTnI on a test line of a paper fluidic platform. By measuring the SERRS signal from the test line, the concentration of cTnI was quantitatively determined. Results: The aptamer-based SERRS assay on a paper strip had a detection range of 0.016 to 0.1 ng∕ml for cTnI, had good selectivity for cTnI compared to three other markers, had good stability over 10 days, and had good performance in the more complex serum sample matrix. Conclusions: The aptamer-based SERRS assay on a paper strip has the potential to provide a sensitive, selective, stable, repeatable, and cost-effective platform for the detection of cTnI toward eventual use in diagnosis of MI at the POC.

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Enzyme-free immunoassay for rapid, sensitive, and selective detection of C-reactive protein

Munusamy,

Zheng,

Jahani

et al. 2024

Anal Bioanal Chem

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A paper microfluidics-based fluorescent lateral flow immunoassay for point-of-care diagnostics of non-communicable diseases

Abstract: An easy-to-use, low-cost and portable fluorescent lateral flow immunoassay based on paper microfluidics was developed for the point-of-care diagnosis of non-communicable diseases.

Cited by 24 publications

References 52 publications

A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I

A Cellulose Paper-Based Fluorescent Lateral Flow Immunoassay for the Quantitative Detection of Cardiac Troponin I

Aptamer-based surface-enhanced resonance Raman scattering assay on a paper fluidic platform for detection of cardiac troponin I

Enzyme-free immunoassay for rapid, sensitive, and selective detection of C-reactive protein

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