a b s t r a c tSpatiotemporally resolved wall heat transfer measurements can provide valuable insight into the fundamental mechanisms affecting flow boiling in microchannels. Operating at the microscale, necessitates resolving changes in local and instantaneous heat transfer characteristics on the order of 100 lm and 1 kHz, respectively. Straightforward interpretation of transient temperature measurements is often challenging due to the conjugate conduction effects in the substrate, which can dampen the measured and inferred heat transfer quantities. These damping effects are described using a slip coefficient (S), which represents the fraction of the change in the local heat transfer that is registered by a sensor (negligible thermal mass) located on a given substrate. Using S, arguments are presented that the conduction patterns in the substrate are predominantly 1-D (i.e. into the substrate) at suitable spatiotemporal-scales. Building on these fundamental considerations, a numerical procedure is adopted to allow a time varying estimate of the local convective heat flux and heat transfer coefficient from transient temperature measurements. Examples of this framework are showcased with experimental results and discussions for interactions observed during flow boiling of HFE 7000 in a single microchannel (hydraulic diameter = 370 lm). At the relatively low mass flux of 200 kg/m 2 s reported in this work, liquid evaporation was found to dictate the local heat transfer trends. High rates of heat transfer were observed to accompany the growth of bubbles and evaporation of the liquid film under vapor slugs. Local dryout was routinely observed in the bubbly and slug flow regime and found to initially enhance heat transfer (i.e. at the creation and subsequent propagation of the three-phase contact line) and present near-zero heat transfer rates in the dried-out domain.