A Partial Genetic Map of Stylosanthes Developed Using Inter-Specific F2 Cross

Abstract

Summary Low polymorphism in Stylosanthes emphasizes use of inter-specific crosses for the development of mapping population for genetic linkage map. Using 78 F 2 individuals of the cross of S. scabra cv. Seca and S. fruticosa, 7 linkage groups were obtained when 81 polymorphic RAPD (Random Amplified Polymorphic DNA) markers were processed. Of these, 53 segregated in expected ratio of 3 : 1 for dominant marker in the F 2 generation, 11 markers in 1 : 1 while 17 deviated from the expected ratio. Thirty markers remain unlinked. Total genome length covered by markers in the present partial map is 630.4 cM (centi Morgan) with an average interval of 39.4 cM. The skeleton map developed is being used to place reasonable numbers of RAPD and STS (Sequence Tagged Sites) markers and to identify QTLs (Quantitative Trait Loci) for anthracnose disease, one of the most important disease of the genus Stylosanthes.

Monitoring of cell wall modifications during callogenesis in Stylosanthes guianensis (Fabaceae) under salt stress conditions

“…Due to their vigorous growth habit, deep rooting ability, and resistance to poor and infertile soils, stylosanthes species, associated with a wide range of grasses, are currently being used as protein banks and green manure and as forage crops (Partridge 1996;Lovato and Martins 1997). Also, they are currently used as feed for pasturing cattle and as a component for wasteland reclamation (Chandra 2006).…”