A Pathologist's Odyssey

Karnovsky, Morris J.

doi:10.1146/annurev.pathol.1.110304.100140

Cited by 7 publications

(1 citation statement)

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“…Morris Karnovsky who actually deserves the most credit for DAB, as he initially introduced it for the cytochemistry of peroxidase, was invited in 1994 to the 36th annual meeting of the Society for Histochemistry in Heidelberg and delivered the Feulgen Lecture with the topic "Cytochemistry and Reactive Oxygen Species: a Retrospective" (Karnovsky 1994). He continues as an active scientist and still enjoys his odysseys not only in the world of sciences (Karnovsky 2006).…”

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confidence: 99%

Peroxisomes: 40 years of histochemical staining, personal reminiscences

Fahimi

2009

Histochem Cell Biol

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The historical circumstances that led to the discovery of the 3,3'-diamino-benzidine (DAB) method for staining of peroxisomes 40 years ago are reviewed. In the course of studies on the uptake and absorption of horse radish peroxidase in mammalian liver, in sections incubated for detection of peroxidase activity in DAB, it was noted that peroxisomes also stained positively for peroxidase activity. Subsequently, it was revealed that the peroxidatic activity of catalase, which is abundantly present in peroxisomes, is responsible for that staining. This notion was confirmed in quantitative biochemical studies with crystalline beef liver catalase and in tracer studies using catalase as an ultrastructural tracer. The application of the DAB method led to the discovery of peroxisomes as a ubiquitous eukaryotic cell organelle, attracting great interest in their investigation in biomedical research.

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confidence: 99%