A pattern for gastric emptying in mice

Abstract

Gastric emptying was studied in CD-1 mice weighing 15-30 g. Mice were fed a nonabsorbable test meal consisting of radioactive barium sulfate suspended in 1.5% methylcellulose. The amount of test meal remaining in the stomach was determined 1, 2, 5, and 10 min after the administration. The initial rates of emptying were significantly higher in the groups weighing 15-20 g body wt. In all groups, 50% of the radioactivity administered was emptied in less than 5 min. The gastric emptying figures obtained were adjusted to three general equations: exponential, hyperbolic, and parabolic. The best correlation coefficients were obtained with the parabola, although this pattern of emptying is not completely satisfactory because it has a minimum before the 10th min. The comparison of the exponential and hyperbolic patterns, in terms of both the correlation coefficients and the "starting indexes," showed that the hyperbola describes the data more closely. No sex differences were observed.

Resolvase-In Vivo Expression Technology Analysis of theSalmonella entericaSerovar Typhimurium PhoP and PmrA Regulons in BALB/c Mice

“…Moreover, in vitro experiments with transient pH pulses did not show marked induction of pagP or pmrH. Based on data from the literature, the emptying kinetics of the stomach in young mice follows an exponential decay function, and the half-emptying time with phenol red in saline solution is about 8 min (28), while it is less than 5 min for a BaSO 4 meal (37). Similarly, in the duodenum, transit time is approximately 1 min (39).…”

Characterization of the Urease Operon of Brucella abortus and Assessment of Its Role in Virulence of the Bacterium

“…The same pattern of acid sensitivity in vitro and attenuation in the mouse oral model has been observed for ureC1 mutants of B. abortus S19, B. melitensis Rev1, and B. suis 1330 (unpublished results). The transit time through the stomach of a liquid-fed mouse can be estimated to be 15 min (26). Thus, the acid exposure conditions used in this work were milder than the acid stress conditions experienced by Brucella during passage through the stomach of a healthy human, which include a pH of less than 3 and a gastric transit time of 2 h (11), or through the abomasum of a ruminant (41).…”

A non‐invasive method for measurement of gastric emptying in mice: effects of altering fat content and CCK A receptor blockade

“…This method was first demonstrated in rats 1 and has since been modified for use in mice 2–4 . Other variations of this method are those that include the measurement of the passage of a radiolabelled solid meal 5–7 or marker dilution studies using a non‐absorbable dye 8–10 . Time‐course studies are highly effective, but are also resource‐ and labour‐intensive, invasive, stressful, and ultimately non‐survival.…”