A PCR-Based Epidemiological Survey of Hepatozoon canis in Dogs in Nigeria

Sasaki, Mizuki; Omobowale, Temidayo Olutayo; Ohta, Kaisaku; Tozuka, Morito; Matsuu, Aya; Hirata, Haruyuki; Nottidge, Helen Oyebukola; Ikadai, Hiromi; Oyamada, Takashi

doi:10.1292/jvms.70.743

Cited by 19 publications

(13 citation statements)

References 12 publications

(23 reference statements)

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“…(JEFFERIES et al, 2003), showed a band that was about 500 bp greater than the Babesia band, and was also able to detect Hepatozoon species (O'DWYER et al, 2009). Similar results were obtained by Oyamada et al (2005) and Sasaki et al (2008).…”

Section: Molecular Diagnosissupporting

confidence: 78%

Brazilian canine hepatozoonosis

O’Dwyer

2011

Rev. Bras. Parasitol. Vet.

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The genus Hepatozoon includes hundreds of species that infect birds, reptiles, amphibians and mammals, in all continents with tropical and subtropical climates. Two species have been described in domestic dogs: H. canis, reported in Europe, Asia, Africa, South America and the United States; and H. americanum, which so far has only been diagnosed in the United States. In Brazil, the only species found infecting dogs is H. canis. The objective of this review was to detail some aspects of canine hepatozoonosis, caused by H. canis, and the main points of its biology, transmission, pathogenicity, symptoms, epidemiology and diagnostic methods, with emphasis on research developed in Brazil.

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Section: Molecular Diagnosissupporting

confidence: 78%

Brazilian canine hepatozoonosis

O’Dwyer

2011

Rev. Bras. Parasitol. Vet.

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“…In the present study, cytological analysis of the blood samples yielded negative results for H. canis infection, which was similar to that reported by Sasaki et al (2008), who did not detected H. canis gamonts in the blood smears of 400 dogs. Absence of H. canis gamonts in the blood smears may be because of the low sensitivity of the technique and/or because of low or intermittent parasitemia in the dogs in this region (Baneth et al, 1998).…”

Section: Discussionsupporting

confidence: 92%

Canine hepatozoonosis in southeastern Bahia, Brazil

et al. 2016

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ABSTRACT. In Brazil, canine hepatozoonosis is a tick-borne subclinical hemoparasitosis caused by a protozoa Hepatozoon canis and is highly prevalent in dogs in rural areas. An epizootiological study was conducted to investigate the prevalence of H. canis in the canine population of Ituberá, Bahia, and to analyze any associated risk factors. Blood samples were collected from 380 dogs and determined the presence of the protozoan by performing capillary blood smear and polymerase chain reaction (PCR). Epizootiological data were collected by asking dog owners to answer a structured questionnaire. H. canis gamonts were not detected in the blood smears. However, PCR detected H. canis in 163/380 (42.9%) dogs examined. Physical examination and anamnesis indicated 105 (64.4%) positive asymptomatic dogs. Hematological alterations were observed in 115 (70.5%) infected dogs. No clinical, hematological, or epizootiological variable was found to be significantly associated to the infection. In conclusion, the high prevalence of H. canis infection in local dogs may be because of the peri-urban features of this municipality. Moreover, to the best of our knowledge, this study the first study to report H. canis infection in the State of Bahia.

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“…When compared with the results of other studies conducted in Turkey, the prevalence of canine hepatozoonosis was lower in this region than that of Aegean coast and south-eastern regions (Karagenc et al, 2006;Aktas et al, 2013). Higher infection rates were recorded to be as 25.8% by Karagenc et al (2006) in Turkey, 20.3% by Sasaki et al (2008) in Nigeria, 57.8% by Otranto et al (2011) in Italy, 11.4% by Jittapalapong et al (2006) in Thailand, 11.8% by Vojta et al (2009) in Croatia and 79.2% by de Miranda et al (2014) in Brazil. The lower infection rate of H. canis in this study might be attributed to the main vectors, but the studies related with the identification or distribution of the arthropod vectors of H. canis have not been organized in the region yet.…”

Section: Discussionmentioning

confidence: 51%

Molecular detection and characterization of Hepatozoon spp. in dogs from the Central part of Turkey

Aydın

Sevinç

Sevinc

2015

Ticks and Tick-borne Diseases

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Canine hepatozoonosis is a tick-borne protozoal disease caused by Hepatozoon spp. Two species of Hepatozoon are currently known to infect dogs as Hepatozoon canis and H. americanum. Although H. canis generally causes a chronic infection with relatively mild clinical alterations compared to H. americanum, infection by H. canis can be life-threatening. The disease is widespread in USA, Africa, Europe, South America, and Asia. To determine the frequency of infection with Hepatozoon spp. in stray dogs from Central Anatolia Region of Turkey, a total of 221 blood samples collected over a three-year period were evaluated by using genus specific Polymerase Chain Reaction (PCR) designed to amplify a fragment of 666bp located in 18 S rRNA gene of Hepatozoon spp. Eight (3.61%) blood samples were positive for Hepatozoon spp. For the classification of species, all positive PCR products were purified with a PCR purification kit and sequenced. Sequencing results of eight representative amplicons indicated that 6 were 98-99% identical to the sequence of H. canis and the other 2 sequences were 95-97% identical to the sequence of Hepatozoon spp. So it was named Hepatozoon sp. MF. A phylogenetic tree was constructed from the sequences of the tick-borne agents identified previously and in this study using the neighbor-joining method. The nucleotide sequences were compared to the H. canis sequences reported in Turkey using the nucleotide Basic Local Alignment Search Tool (BLAST) program. The results of this study are significant in terms of the presence of a novel canine Hepatozoon genotype.

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A PCR-Based Epidemiological Survey of Hepatozoon canis in Dogs in Nigeria

Cited by 19 publications

References 12 publications

Brazilian canine hepatozoonosis

Brazilian canine hepatozoonosis

Canine hepatozoonosis in southeastern Bahia, Brazil

Molecular detection and characterization of Hepatozoon spp. in dogs from the Central part of Turkey

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