A pendant peptide endows a sunscreen with water-resistance

Ellison, Aubrey J.; Raines, Ronald T.

doi:10.1039/c8ob01773e

Cited by 8 publications

(17 citation statements)

References 40 publications

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“…21 As a CMP, we chose (PPG)7 because of its simplicity and demonstrated efficacy in relevant contexts in vitro, ex vivo, and in vivo. 26,28,29,32,33 In its selection, LTGKNFPMFHRN (Tβrl) was displayed as a fusion to the N-terminus of the PIII coat protein of phage. 21 We mimicked this display by conjugating Tβrl to the N-terminus of CMP.…”

Section: Resultsmentioning

confidence: 99%

“…[23][24][25] We and others have reported on the use of collagenmimetic peptides (CMPs) to anchor probes and growth factors in damaged or abnormal collagen. [26][27][28][29][30][31][32][33][34] Here, we use a CMP conjugate to immobilize a peptidic ligand for TGF-β receptors in wound beds of mice. Our intent is to cluster cell-surface TβRI and TβRII and thereby enhance the sensitivity of cells to circulating TGF-β (Figure 1B).…”

Section: Introductionmentioning

confidence: 99%

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Bifunctional Peptide that Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing

Chattopadhyay

Teixeira

Kiessling

et al. 2021

Preprint

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Transforming growth factor-β (TGF-β) plays important roles in wound healing. The activity of TGF-β is initiated upon binding of the growth factor to extracellular domains of its receptors. We sought to facilitate activation by clustering these extracellular domains. To do so, we used a known peptide that binds to TGF-β receptors without diminishing their affinity for TGF-β. We conjugated this peptide to a collagen-mimetic peptide that can anneal to damaged collagen in a wound bed. We find that the conjugate enhances collagen deposition and wound closure in mice in a manner consistent with the clustering of TGF-β receptors. This strategy provides a means to upregulate the TGF-β signaling pathway without adding exogenous TGF-β and could inspire means to treat severe wounds.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Bifunctional Peptide that Anneals to Damaged Collagen and Clusters TGF-β Receptors Enhances Wound Healing

Chattopadhyay

Teixeira

Kiessling

et al. 2021

Preprint

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“…This conjugate, Red- l CMP, had the sequence Ac-Lys(Rhodamine Red)-(Ser-Gly) 3 -( l -Pro- l -Pro-Gly) 7 . We chose this sequence because a ( l -Pro- l -Pro-Gly) 7 peptide does not form a stable homotrimeric helix ( T m = 6–7 °C) but does anneal to damaged collagen. ,, We used this conjugate to probe the surface of GAS cells. Specifically, we grew bacterial cells for 24 h and incubated them with Red- l CMP for 1 h at 37 °C.…”

Section: Results and Discussionmentioning

confidence: 99%

“…When skin becomes damaged, a human is more vulnerable to infection. In wounds, collagen loses its triple-helical structure, and collagen mimetic peptides (CMPs) can adhere to the damaged collagen. − Hence, we surmised that GAS could likewise use its endogenous collagen-like domains to bind to damaged collagen.…”

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confidence: 99%

Role for Cell-Surface Collagen of Streptococcus pyogenes in Infections

et al. 2020

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Group A Streptococcus (GAS) displays cell-surface proteins that resemble human collagen. We find that a fluorophore-labeled collagen mimetic peptide (CMP) labels GAS cells but not Escherichia coli or Bacillus subtilis cells, which lack such proteins. The CMP likely engages in a heterotrimeric helix with endogenous collagen, as the nonnatural D enantiomer of the CMP does not label GAS cells. To identify a molecular target, we used reverse genetics to "knock-in" the GAS genes that encode two proteins with collagen-like domains, Scl1 and Scl2, into B. subtilis. The fluorescent CMP labels the cells of these B. subtilis strains. Moreover, these strains bind tightly to a surface of mammalian collagen. These data are consistent with streptococcal collagen forming triple helices with damaged collagen in a wound bed, and thus have implications for microbial virulence.

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“…Such annealing could allow for the development of diagonistic or therapeutic agents conjugated to a monomeric CMP. [19][20][21][22] Previously, we have used CMP-conjugates to deliver fluorescent dyes, 23 a sunscreen, 24 and growth factors 25 to damaged collagen. Although (flpFlpGly) 7 seemed to outperform (ProProGly) 7 as an annealing strand, 23 its exceptional propensity was hindered by its poor solubility, which can complicate formulation and dosing, and diminish bioavailability.…”

Section: Introductionmentioning

confidence: 99%