A Peptide‐Based Electrochemical Biosensor for Facile Measurement of Whole‐Blood Heparin

Meng, Fanyu; Liang, Weiguo; Sun, Haixuan; Wu, Ligang; Gong, Xiao

doi:10.1002/celc.201600680

Cited by 16 publications

(8 citation statements)

References 25 publications

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“…Direct quantification of polyions is known to be challenging because a large number of polyions do not have intrinsic visible absorption, fluorescent, or redox active units. Rather, oppositely charged dyes or nanoparticle dyes, oppositely charged redox active molecules, oppositely charged polymers, or oppositely charged surfaces are often used to indirectly indicate the levels of polyions present in given samples via optical methods (e.g., spectrophotometry, fluorimetry, surface plasmon resonance spectroscopy, Raman spectroscopy), − electrochemical methods (e.g., voltammetry, impedimetry), − and other techniques such as quartz crystal microgravimetry. , However, because of tedious protocols, required instrumentation, and interference from sample matrix components, these methods have had limited applications.…”

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confidence: 99%

Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

2017

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The first paper-based polyion-sensitive optodes are reported. Dinonylnaphthalene sulfonic acid (a cation exchanger) and chromoionophore I (a lipophilic optical pH indicator) are printed on filter paper in the absence of any plasticizer and/or additional hydrophobic polymeric phase. The resulting optodes exhibit sensitive colorimetric response to polycations such as protamine but not to small inorganic cations because only polycations are able to form cooperative ion pairs with dinonylnaphthalenesulfonate adsorbed to the cellulose paper. The color change of the optode is recorded via an iPhone camera and analyzed by an iPhone App. The protamine-sensing optode platform is used to indirectly detect protease activity (trypsin) based on proteolytic digestion of protamine, and polyanions (pentosan polysulfate and heparin) based on the strong binding reaction of polyanions with protamine. The indirect sensing system is further simplified on a multilayer membrane device that consists of an optode paper site modified with buffer to prevent optode dependence on sample pH, and an underlying cellulose acetate filter membrane coated with protamine to eliminate addition of the indicator polycation into the sample. The detection of pentosan polysulfate concentrations in an undiluted urine sample is successfully demonstrated via this approach. Lastly, it is shown that plasticizer-free polyanion-sensitive optodes based on an adsorbed layer of quaternary ammonium type anion exchanger and a phenolic azo type proton chromoionophore can also be fabricated directly on cellulose paper strips.

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confidence: 99%

Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

2017

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“…On the surface of the ITO electrode, (−) CdTe QDs reveals a small Ret (curve a), and addition of (+) AuNPs only causes a slight change of Ret (curve b in Figure A). As nonconductive biomolecules, peptide and protein may give rise to steric hindrance on the surface of an electrode and thus serve as barriers to the interfacial electron transfer. , Accordingly, peptide and protein induces much larger increases of Ret (curves c, d, and e in Figure A). Taken together, the assembly of this PEC analytical system is clearly characterized by the variations of Ret .…”

Section: Resultsmentioning

confidence: 99%

Photoelectrochemical Approach to Apoptosis Evaluation via Multi-Functional Peptide- and Electrostatic Attraction-Guided Excitonic Response

Wang

Liu

et al. 2018

Anal. Chem.

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The excitonic response between nanomaterials is distance-dependent, and thus, interparticle distance is a key factor in fabricating diverse photoelectrochemical (PEC) systems. Current studies focus on DNA-mediated regulation of interparticle distance. However, limited by high demands of base-pairing and flexibility of DNA, it is hard for DNA to achieve precise regulation, especially in a short distance. To pursue better PEC performances in bioanalyses, alternative biological materials should be explored to replace DNA as new “distance controllers”. In this work, a peptide with three functional sequences is designed to control interparticle distance between positive-charged Au nanoparticles ((+) AuNPs) and negative-charged CdTe quantum dots ((−) CdTe QDs). Relying on the function of binding sequence, (+) AuNPs and (−) CdTe QDs may be separated to a certain distance by the multifunctional peptide. In this case, the excitonic response is relatively weak, and an evident PEC response can be observed. Because it contains the substrate sequence of caspase-3, the peptide is cleaved in the presence of caspase-3. As a result, without the support of intact peptide, electrostatic attraction plays a dominant role, leading to the aggregation of oppositely charged AuNPs and CdTe QDs, which strengthens the excitonic response and attenuates the PEC response. On the basis of these principles, a novel PEC approach was fabricated to sensitively quantify caspase-3. Meanwhile, caspase-3 in staurosporine-treated A549 cells are also determined by the approach, and the obtained results agree well with the fluorescent intensity of confocal images, manifesting that the proposed PEC method can monitor apoptosis in a label-free strategy. Overall, the study reveals the capability of peptides in controlling interparticle distance of nanomaterials, which may accelerate the development of peptide-based PEC analytical methods.

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“…The DNA tetrahedron product was slowly cooled to room temperature and stored in 4 °C until use. The substrate gold electrode was cleaned as described in a previous literature . Next, 10 μL of DNA tetrahedron was dipped on the pretreated electrode.…”

Section: Methodsmentioning

confidence: 99%

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

Wang

Zhou

2017

ChemElectroChem

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Based on the catalysis of exonuclease I (Exo I) and nicking endonuclease (NEase), a novel electrochemical biosensor for the detection of coralyne is fabricated in this work. Specifically, DNA probe 1 can be protected from Exo I‐catalyzed digestion upon interacting with coralyne, the most representative homo‐adenine binding molecule. Subsequently, DNA probe 1 can further hybridize with a pendant sequence on top of the DNA tetrahedron, which is previously modified on the gold electrode surface. As the formed duplex contains the recognition site of Nb.BssSI, a type of NEase, the pendant sequence is thus cleaved, releasing the electrochemical probe on the 5’‐terminus from the electrode surface. DNA probe 1 is also released, which could then initiate the subsequent cycles of NEase‐catalyzed cleavage and amplify the declined electrochemical signals. We can determine coralyne in a linear range from 0.1 to 100 nM with the detection limit of 0.07 nM by using the proposed method. Experiments in real samples also demonstrate good results, showing excellent practical utility of this method. Moreover, it could be extended to assay other analytes by modifying certain DNA sequences.

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A Peptide‐Based Electrochemical Biosensor for Facile Measurement of Whole‐Blood Heparin

Cited by 16 publications

References 25 publications

Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

Inkjet-Printed Paper-Based Colorimetric Polyion Sensor Using a Smartphone as a Detector

Photoelectrochemical Approach to Apoptosis Evaluation via Multi-Functional Peptide- and Electrostatic Attraction-Guided Excitonic Response

Exonuclease and Nicking Endonuclease‐Assisted Amplified Electrochemical Detection of Coralyne

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