A peptide-DNA hybrid bio-nanomicelle and its application for detection of caspase-3 activity

Zhang, Fan; Mao, Changqing; Cao, Siyu; Zhang, Runchi; Guo, Yi; Chen, Guifang; Feng, Chao

doi:10.3389/fchem.2022.1005315

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…This facilitated the release of a fluorophore at the ends of the DNA, leading to fluorescent measurements. Through this fluorescence‐based strategy, the peptide‐DNA hybrid could selectively and specifically detect caspase‐3 activity via peptide‐substrate cleavage [109] . Park et al [111] .…”

Section: Applications Of Peptide Functionalized Dna Hybrid Nanostruct...mentioning

confidence: 99%

“…[106] Peptide-DNA hybrids also show excellent selectivity and sensitivity, paving the way for molecule recognition applications. Feng et al, [109] developed a peptide-DNA bio-nanomicelle for the detection of caspase-3, an important protein that acts as a biomarker for apoptosis. [109,110] Exploiting electrostatic interactions between negatively charged DNA and positively charged peptides, nanomicelles of certain sizes were formed, guided by the hydrophobic function of a cholesterol group attached to the DNA sequence.…”

Section: Self-assembled Dna-peptide Structures For Cellular Instructi...mentioning

confidence: 99%

“…Through this fluorescence-based strategy, the peptide-DNA hybrid could selectively and specifically detect caspase-3 activity via peptide-substrate cleavage. [109] Park et al [111] used an azide-terminated peptide and alkyneterminated DNA to form diblock and triblock peptide-DNA complexes that assembled into nanosheets. The nanosheets had DNA and peptide on opposite faces, due to which each face had a distinct chemical and functional identity.…”

Section: Self-assembled Dna-peptide Structures For Cellular Instructi...mentioning

confidence: 99%

“…Feng et al, [109] developed a peptide-DNA bio-nanomicelle for the detection of caspase-3, an important protein that acts as a biomarker for apoptosis. [109,110] Exploiting electrostatic interactions between negatively charged DNA and positively charged peptides, nanomicelles of certain sizes were formed, guided by the hydrophobic function of a cholesterol group attached to the DNA sequence. This nanomicelle was observed to be stable in vitro and cleaved an Asp-Glu-Val-Asp peptide substrate in the presence of caspase-3.…”

Section: Self-assembled Dna-peptide Structures For Cellular Instructi...mentioning

confidence: 99%

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Designer, Programmable DNA‐peptide hybrid materials with emergent properties to probe and modulate biological systems

et al. 2023

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The chemistry of DNA endows it with certain functional properties that facilitate the generation of self-assembled nanostructures, offering precise control over their geometry and morphology, that can be exploited for advanced biological applications. Despite the structural promise of these materials, their applications are limited owing to lack of functional capability to interact favourably with biological systems, which has been achieved by functional proteins or peptides. Herein, we outline a strategy for functionalizing DNA structures with short-peptides, leading to the formation of DNA-peptide hybrid materials. This proposition offers the opportunity to leverage the unique advantages of each of these bio-molecules, that have far reaching emergent properties in terms of better cellular interactions and uptake, better stability in biological media, an acceptable and programmable immune response and high bioactive molecule loading capacities. We discuss the synthetic strategies for the formation of these materials, namely, solid-phase functionalization and solution-coupling functionalization. We then proceed to highlight selected biological applications of these materials in the domains of cell instruction & molecular recognition, gene delivery, drug delivery and bone & tissue regeneration. We conclude with discussions shedding light on the challenges that these materials pose and offer our insights on future directions of peptide-DNA research for targeted biomedical applications.

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Section: Applications Of Peptide Functionalized Dna Hybrid Nanostruct...mentioning

confidence: 99%

Section: Self-assembled Dna-peptide Structures For Cellular Instructi...mentioning

confidence: 99%

Section: Self-assembled Dna-peptide Structures For Cellular Instructi...mentioning

confidence: 99%

Section: Self-assembled Dna-peptide Structures For Cellular Instructi...mentioning

confidence: 99%

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Designer, Programmable DNA‐peptide hybrid materials with emergent properties to probe and modulate biological systems

et al. 2023

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“…nanomicelle for the activity detection of caspase-3 (Zhang et al, 2022). They utilized the caspase-3 specific recognition and cleavage of peptide substrates to achieve high sensitivity and selectivity.…”

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confidence: 99%

Editorial: Disease biomarker analysis based on optical biosensing

et al. 2023

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Editorial on the Research Topic Disease biomarker analysis based on optical biosensingDisease biomarker analysis has become a crucial tool for diagnosing and evaluating disease prognosis, especially with the increasing understanding of diseases at the molecular level. Abnormalities in various biomarkers can indicate diseased states, and can be used to rapidly and specifically detect and quantify diseases using optical biosensing techniques (Gao et al., 2023). Optical biosensing techniques have several advantages over traditional methods including higher sensitivity, specificity, and faster analysis times (Plikusiene and Ramanaviciene, 2023). It also allows for non-invasive sample collection. With advancements in optical biosensing technology, many medical conditions including cancers, infectious diseases, and autoimmune disorders can be accurately diagnosed and efficiently treated (Singh et al., 2023;Tang et al., 2023). The combination of optical biosensing with emerging technologies such as material science, optics, and electronics has further accelerated its development in biomarker analysis (Qureshi et al., 2022). Interdisciplinary collaboration between experts in fields such as physics, chemistry, bioengineering, and medicine has helped pave the way for novel optical biosensing technologies as well as improving existing ones. Continued interdisciplinary collaboration is essential in advancing the field of disease biomarker analysis based on optical biosensing. This exciting area of research holds great potential for the future of personalized and precision medicine, and will likely lead to more effective disease diagnoses and treatments (Duo et al., 2023).In order to highlight the most up-to-date research in this field, a Research Topic titled "Disease Biomarker Analysis Based on Optical Biosensing" was launched in Analytical Chemistry in Frontiers. The Research Topic consists of a total of 5 articles, which include 3 original research articles and 2 review articles. Through these publications, novel insights and significant discoveries are presented in the area of optical biosensing, contributing greatly to the advancement of this rapidly evolving field.Bio-nanomicelles based on biomaterials such as nucleic acids, peptides, glycans, and lipids have rapidly developed in the field of bioanalysis (Yang et al., 2020). However, few bionanomicelles integrate DNA with peptides, which can provide unique advantages for biomedical applications. In light of this, Feng et al. designed a peptide-DNA hybrid bio-

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Visual monitoring of cisplatin-regulated caspase-3 activity in living cells based on a reduced graphene oxide-loaded fluorescent probe

Liu,

Zhou,

Zhang

et al. 2024

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A peptide-DNA hybrid bio-nanomicelle and its application for detection of caspase-3 activity

Cited by 3 publications

References 26 publications

Designer, Programmable DNA‐peptide hybrid materials with emergent properties to probe and modulate biological systems

Designer, Programmable DNA‐peptide hybrid materials with emergent properties to probe and modulate biological systems

Editorial: Disease biomarker analysis based on optical biosensing

Visual monitoring of cisplatin-regulated caspase-3 activity in living cells based on a reduced graphene oxide-loaded fluorescent probe

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