A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells

Mattos, B. B.; Montebianco, Caroline de Barros; Romanel, Elisson; Silva, Tatiane da Franca; Bernabé, Renato Barroso; Simas, Fernanda Fogagnoli; Souza, Lauro Mera de; Sassaki, Guilherme L.; Vaslin, Maite F S; Barreto‐Bergter, Eliana

doi:10.1016/j.plaphy.2018.02.023

Cited by 11 publications

(17 citation statements)

References 43 publications

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“…The BY-2 protoplast is proved to be an effective system for agents screening and clarification of their mode of action. For example, BY-2 cells were treated with microbial-derived metabolite from Cladosporium herbarum , which results in significant up-regulation of host defense-related genes [66]. In this study, our results showed that NNM treatment effectively inhibited viral RNA accumulation of TMV and significantly regulated many lines of potential virus resistance genes in the tobacco BY-2 protoplasts.…”

Section: Discussionmentioning

confidence: 64%

Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Zhou

Guo

et al. 2019

Viruses

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Ningnanmycin (NNM) belongs to microbial pesticides that display comprehensive antiviral activity against plant viruses. NNM treatment has been shown to efficiently delay or suppress the disease symptoms caused by tobacco mosaic virus (TMV) infection in local-inoculated or systemic-uninoculated tobacco leaves, respectively. However, the underlying molecular mechanism of NNM-mediated antiviral activity remains to be further elucidated. In this study, 414 differentially expressed genes (DEGs), including 383 which were up-regulated and 31 down-regulated, caused by NNM treatment in TMV-infected BY-2 protoplasts, were discovered by RNA-seq. In addition, KEGG analysis indicated significant enrichment of DEGs in the plant–pathogen interaction and MAPK signaling pathway. The up-regulated expression of crucial DEGs, including defense-responsive genes, such as the receptor-like kinase FLS2, RLK1, and the mitogen-activated protein kinase kinase kinase MAPKKK, calcium signaling genes, such as the calcium-binding protein CML19, as well as phytohormone responsive genes, such as the WRKY transcription factors WRKY40 and WRKY70, were confirmed by RT-qPCR. These findings provided valuable insights into the antiviral mechanisms of NNM, which indicated that the agent induces tobacco systemic resistance against TMV via activating multiple plant defense signaling pathways.

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Section: Discussionmentioning

confidence: 64%

Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Zhou

Guo

et al. 2019

Viruses

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“…Livak and Schmitgen [21]. Primers used for qPCR analysis were described by Mattos et al, 2018. Annealing temperature of all genes evaluated was 60˚C, except for PAL and PR5, whose annealing temperature was 62˚C.…”

Section: Plos Onementioning

confidence: 99%

“…PR-1 (pathogenesis related protein-1 with suggested antifungal action), PR-2 (β-1,3-glucanase), PR-3 (chitinase), PR-5 (osmotin/thaumatin-like), PAL (phenylalanine ammonia-lyase), LOX (lipoxygenase) and POX (Peroxidase) genes expression was evaluated. PP2A (phosphatase 2A) and Ntbuc (ubiquitin) were used to normalize cDNA expression levels [22].…”

Section: Plos Onementioning

confidence: 99%

Antiviral activity of glucosylceramides isolated from Fusarium oxysporum against Tobacco mosaic virus infection

et al. 2020

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Natural elicitors derived from pathogenic microorganisms represent an ecologic strategy to achieve resistance in plants against diseases. Glucosylceramides (GlcCer) are classified as neutral glycosphingolipids. GlcCer were isolated and purified from Fusarium oxysporum mycelium. F. oxysporum is a plant pathogenic fungus, abundant in soil and causing severe losses in economically important crops such as corn, tobacco, banana, cotton and passion fruit. In this study we evaluate the capacity of GlcCer in inducing resistance in N. tabacum cv Xanthi plants against Tobacco mosaic virus (TMV). Spraying tobacco plants with GlcCer before virus infection reduced the incidence of necrotic lesions caused by TMV. In addition, plants already infected with the virus showed a reduction in hypersensitive response (HR) lesions after GlcCer treatment, suggesting an antiviral effect of GlcCer. Our investigations showed that GlcCer stimulates the early accumulation of H2O2 and superoxide radicals. In addition, the expression of PR-1 (pathogenesis-related 1, with suggested antifungal action), PR-2 (β-1,3-glucanase), PR-3 (Chitinase), PR-5 (Osmotin), PAL (Phenylalanine ammonia-lyase), LOX (Lipoxygenase) and POX (Peroxidase) genes was highly induced after treatment of tobacco plants with GlcCer and induction levels remained high throughout a period of 6 to 120 hours. Our experiments demonstrate that GlcCer induces resistance in tobacco plants against infection by TMV.

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“…cDNA amplification reactions were performed in a final volume of 25 μl, according to the manufacturer's guidelines. qPCR cycles were 10 minutes at 95°C for initial denaturation, followed by 40 cycles of denaturation at 95°C for 15 sec and annealing/extension at 60°C for 1 minute, except for the PAL and PR5 genes, for which the annealing temperature was adjusted to 62°C [23]. The results were analyzed by the 2 −△△ CT method according to [29].…”

Section: Analysis Of Defense Gene Expression By Qrt-pcrmentioning

confidence: 99%

“…β-D-Galactofuranosyl residues were present as (1→5)-interlinked side chains of C. herbarum pGM. The role of pGM cell wall glycoprotein in plant-fungus interactions was first studied by Mattos et al [23], who showed the induction of the expression of defense genes in tobacco BY2 cells and a hypersensitive response (HR) after treatment of tobacco leaves with C. herbarum pGM. This recognition and defense response activation would possibly contribute to the plant response to the pathogen attack, protecting itself against new infections and indicating that pGM is probably able to induce SAR.…”

Section: Introductionmentioning

confidence: 99%

Cladosporium herbarumpeptidogalactomannan triggers significant defense responses in whole tobacco plants

et al. 2020

Preprint

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Cladosporium herbarum is one of the most frequently occurring fungal species, with a worldwide distribution, and is found in almost all man-occupied niches in organic and inorganic matter and as a phytopathogen on certain agricultural crops. The structure of the most abundant glycoprotein from the C. herbarum cell wall, peptidogalactomanann or pGM, was previously elucidated and includes carbohydrates (76%), with mannose, galactose and glucose as its main monosaccharides (52:36:12 molar ratio). pGM was able to strongly induce the expression of defense-related genes and ROS accumulation when in contact with BY2 tobacco cells. Here, using two distinct Nicotiana tabacum cultivars, Xanthi and SR1, we evaluated the ability of C. herbarum pGM to induce SAR-like defense by studying its antiviral activity against Tobacco mosaic virus (TMV) and the induction of SAR markers including PR genes and ROS accumulation. Our results show that pGM induced a strong activation of defense responses in treated plants from both tobacco cultivars, contributing to the impairment of viral infection. Expression levels of the pathogenesis-related genes PR-1a (unknown function), PR-2 (□-1-3 endoglucanase) PR-3 (chitinase), and PR-5 (thaumatin-like protein), the phenylpropanoid pathway gene PAL (phenylalanine ammonia-lyase) and genes involved in plant stress responses and innate immunity, such as LOX1 (lipoxygenase) and NtPrxN1 (peroxidase), were strongly induced until 120 h after pGM spray application. Accumulation of superoxide radicals was also observed in a pGM dose-dependent manner.

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A peptidogalactomannan isolated from Cladosporium herbarum induces defense-related genes in BY-2 tobacco cells

Cited by 11 publications

References 43 publications

Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Molecular Regulation of Host Defense Responses Mediated by Biological Anti-TMV Agent Ningnanmycin

Antiviral activity of glucosylceramides isolated from Fusarium oxysporum against Tobacco mosaic virus infection

Cladosporium herbarumpeptidogalactomannan triggers significant defense responses in whole tobacco plants

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