A performance improvement of enzyme-based electrochemical lactate sensor fabricated by electroplating novel PdCu mediator on a laser induced graphene electrode

Han, Jiwon; Park, Sang Hyun; Kim, Saeyoung; Pak, James Jungho

doi:10.1016/j.bioelechem.2022.108259

Cited by 18 publications

(6 citation statements)

References 57 publications

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“…5 D). 34,35 The increase in electrode surface area contributed to the higher peak current values obtained with the modified electrode.…”

Section: Resultsmentioning

confidence: 99%

“…The geometric area of the working electrode was 0.16π cm 2 . 34,35 Antibody immobilization on the electrode surface and antigen detection was performed using 5.5 μg of IgY anti-rBlo t 5 (5 μl in PBS) added to the work surface of the stabilized electrode and, for reading, we waited for the sample to be completely dry (5 min at RT). For antibody fixation, a CV reading at 0.1 V s −1 was taken, then the electrode was washed only once with 1000 μl of ultra-high purity water type 1, dried with air jets and stored at RT until the next step.…”

Section: Methodsmentioning

confidence: 99%

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A Novel Electrochemical Immunosensor Based on Anti-IgY and PLA/PEG Nanofibrous Mats Used for Allergens Detection in Farinaceous Products

Alves

Notário

Correia

et al. 2023

J. Electrochem. Soc.

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The present study focuses on the first electrochemical immunosensor built with polymeric mats enriched with nanomaterials, targeting the dust mite protein, for the detection of allergens in flour sources with important implications in allergic reactions. As a proof-of-concept, we have used the Blo t 5 allergen from Blomia tropicalis (target) and established electrochemical parameters to recognize and detect the specific allergen using a polyclonal immunoglobulin (Ig) Y (probe). Detection was performed in a portable potentiostat (EmStat) using a graphite screen-printed electrode. For that, the nanofibrous mats of poly(lactic acid)/poly(ethylene glycol), and carbon nanotubes were placed in contact with the surface of the electrodes. The functionalization that occurs through the deposition of the polymer was induced through the passage of current. Subsequently, the modification was validated by cyclic voltammetry (CV) readings and ferri/ferrocyanide was used as a redox indicator in CV analyses. The novel immunosensor was able to discriminate between allergen-contaminated and uncontaminated farinaceous samples. The immunosensor displays high sensitivity detecting up to 50 µg/ml in the calibration curve with a linear response between 5 and 500 µg/ml. The new biosensor shows great potential in the detection of farinaceous contaminants in laboratories, food factories and in the field.

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“…5 D). 34,35 The increase in electrode surface area contributed to the higher peak current values obtained with the modified electrode.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A Novel Electrochemical Immunosensor Based on Anti-IgY and PLA/PEG Nanofibrous Mats Used for Allergens Detection in Farinaceous Products

Alves

Notário

Correia

et al. 2023

J. Electrochem. Soc.

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“… 24 LOx/Pt-chitosan/LIG 0.2–3 mM 35.8 110 Han et al. 25 LOx/PdCu/LIG 0.1–30 mM 51.91 – Tsou et al. 26 LOx/NiOx-Nafion 0.5–4 mM 205.6 270 Neampet et al.…”

Section: Resultsmentioning

confidence: 99%

Continuous monitoring of multiple biomarkers with an ultrasensitive 3D-structured wearable biosensor

Yang,

Wang,

Cheng

2023

Cell Reports Methods

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“…The example of using the LOx/PdCu nanoparticles/laser-induced graphene electrodes could show a LOD of 0.28 μM and the detection ranges over 0.1-5 mM (the low linear range) and 5-30 mM (the second linear range). [133] Additionally, lactate sensors have been made available commercially by various companies such as BSX insight, Sport-Tec, and AES. Depending on product design and sensing technology, a wearable lactate sensor can cost between $300 and $600.…”

Section: Conclusion and Prospectsmentioning

confidence: 99%

“…As an alternative to PB‐based enzymatic electrodes, metallic catalysts (such as the PdCu‐based composite) are also of interest. The example of using the LOx/PdCu nanoparticles/laser‐induced graphene electrodes could show a LOD of 0.28 μM and the detection ranges over 0.1–5 mM (the low linear range) and 5–30 mM (the second linear range) [133] …”

Section: Conclusion and Prospectsmentioning

confidence: 99%

Wearable Electrodes for Lactate: Applications in Enzyme‐Based Sensors and Energy Biodevices

et al. 2023

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Wearable bioelectronics is a promising next-generation technology for its versatility in personalized applications. Measuring lactate is one of the growing trends in wearable biosensing research. To achieve this goal, enzymes capable of catalyzing reactions involving lactate must be coupled with bioelectrode components, creating a variety of biodevices such as biosensors, biofuel cells, and other devices harvesting energy from wearers. This review provides a brief history of noninvasive and minimally invasive enzyme-based lactate biosensors and energy biodevices. We introduce key principles of lactate oxidase and lactate dehydrogenase, together with immobilization strategies for efficient electrical contacts between redox enzymes and electrode supports. Additionally, we discuss recent examples of advanced wearable enzymatic lactate sensors and elaborate on a collection of self-powered wearable energy biodevices (e. g., biofuel cells, triboelectric nanogenerators, and piezoelectric devices). Lastly, we finish this review with discussions on challenges in developing lactate bioelectronics and provide our outlook on the prospects and future directions of this compelling technology.

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A performance improvement of enzyme-based electrochemical lactate sensor fabricated by electroplating novel PdCu mediator on a laser induced graphene electrode

Cited by 18 publications

References 57 publications

A Novel Electrochemical Immunosensor Based on Anti-IgY and PLA/PEG Nanofibrous Mats Used for Allergens Detection in Farinaceous Products

A Novel Electrochemical Immunosensor Based on Anti-IgY and PLA/PEG Nanofibrous Mats Used for Allergens Detection in Farinaceous Products

Continuous monitoring of multiple biomarkers with an ultrasensitive 3D-structured wearable biosensor

Wearable Electrodes for Lactate: Applications in Enzyme‐Based Sensors and Energy Biodevices

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