Biogeochemical reactions in coastal aquifers highly affect the nutrient flux associated with submarine groundwater discharge (SGD) to the ocean, which subsequently influences the oceanic environment and ecology. This study investigates a seasonal variation of SGD-derived nutrient flux to the ocean and nutrient biogeochemistry in an intertidal aquifer of Tolo Harbor, Hong Kong. The results show that the inventory of NO x À and PO 4 3À in the intertidal aquifer has a clear seasonality with a large inventory in summer, a small inventory in spring, and a median inventory in autumn and winter, respectively. Differently, the inventory of NH 4 + is large in winter and summer and small in spring and autumn, which results from the coupled effects of seasonal change of both production and removal of NH 4 + in the aquifer. The SGD-derived nutrient (NH 4 + , NO x À , and PO 4 3À ) flux is the highest in summer (271.71, 24.86, and 116.66 mmol·day À1 ·(m coastline) À1 ) and is the lowest in spring (114.83, 1.70, and 20.26 mmol·day À1 ·(m coastline) À1 ). The majority of SGD-derived nutrient flux is supported by the local remineralization of organic matter along with seawater infiltration. In autumn, the recharge of seawater induced by tidal pumping significantly shifts the biogeochemical balance of nutrients and is the major source of groundwater nutrients in the intertidal aquifer. Among the various nutrient fluxes (SGD, river discharge, atmospheric deposition, and benthic sediment diffusion) to Tolo Harbor, SGD-derived PO 4 3À flux is the second major source of seawater PO 4 3À in addition to benthic sediment diffusion. The PO 4 3À loading via SGD is of significance to the primary production in the phosphorus-limited environment in Tolo Harbor. After considering the natural attenuation of nutrients in a sandy/silty beach aquifer, this study suggests the overestimation of SGD-derived nutrient loading estimated previously that simply use average nutrient concentration of fresh SGD endmember and saline SGD endmember as the nutrient concentration of total SGD endmember.Plain Language Summary The biogeochemical reactions in the intertidal aquifer are the crucial influencing factors affecting the groundwater nutrients that are essential for oceanic environment and ecology. Seasonal hydrologic variations are of significance to the biogeochemical reactions in the aquifer and nutrient speciation and concentrations in coastal groundwater. The results of this study indicate that both nutrient inventories in the aquifer and nutrient fluxes to the sea are higher in summer and autumn than winter and spring. By comparing the nutrient flux carried by the submarine groundwater discharge with other pathways, nutrient flux carried by coastal groundwater is a major source of phosphorus in the sea and supports the primary production in the harbor, which can be demonstrated by the positive trend between the nutrient flux and chlorophyll-a concentration in seawater. However, the red tide occurrence in the harbor does not have a similar seasonal tr...