A peroxiredoxin,<scp>PRDX</scp>‐2, is required for insulin secretion and insulin/<scp>IIS</scp>‐dependent regulation of stress resistance and longevity

Oláhová, Monika; Veal, Elizabeth A.

doi:10.1111/acel.12321

Cited by 58 publications

(30 citation statements)

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“…Likewise, several studies of regulation by low levels of ROS identified peroxidases that act in homeostasis as ROS sensors rather than ROS quenchers. For example, the knockout of peroxiredoxin-2 showed that the protein is required for normal levels of insulin secretion in Caenorhabditis elegans (Olahova and Veal, 2015), and the knockout of glutathione peroxidase-1 resulted in improved glucose control in mice (Merry et al, 2016). Intriguingly, regulation by low levels of ROS displays special attributes.…”

Section: Discussionmentioning

confidence: 99%

Redox regulation of PGRL1 at the onset of low light intensity

et al. 2020

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SUMMARY PGR5‐LIKE PHOTOSYNTHETIC PHENOTYPE1 (PGRL1) regulates photosystem I cyclic electron flow which transiently activates non‐photochemical quenching at the onset of light. Here, we show that a disulfide‐based mechanism of PGRL1 regulated this process in vivo at the onset of low light levels. We found that PGRL1 regulation depended on active formation of key regulatory disulfides in the dark, and that PGR5 was required for this activity. The disulfide state of PGRL1 was modulated in plants by counteracting reductive and oxidative components and reached a balanced state that depended on the light level. We propose that the redox regulation of PGRL1 fine‐tunes a timely activation of photosynthesis at the onset of low light.

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Section: Discussionmentioning

confidence: 99%

Redox regulation of PGRL1 at the onset of low light intensity

et al. 2020

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“…SKN-1 also up-regulates expression of two genes that encode glutathione peroxidases. DAF-16 and SKN-1 are also associated with the up-regulation of the PRDX-2 gene that encodes peroxiredoxin in C. elegans (Oláhová and Veal, 2015). Comparative genomics studies have enabled ROS-scavenging enzymes to be identified in PPNs (Abad et al , 2008; Opperman et al , 2008; Bird et al , 2009).…”

Section: Section Ii: Potential Key Regulators Of Oxidative Stress Resmentioning

confidence: 99%

Plant-parasitic nematodes: towards understanding molecular players in stress responses

Gillet

Bournaud

Júnior

et al. 2017

Ann Bot

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Background Plant–parasitic nematode interactions occur within a vast molecular plant immunity network. Following initial contact with the host plant roots, plant-parasitic nematodes (PPNs) activate basal immune responses. Defence priming involves the release in the apoplast of toxic molecules derived from reactive species or secondary metabolism. In turn, PPNs must overcome the poisonous and stressful environment at the plant–nematode interface. The ability of PPNs to escape this first line of plant immunity is crucial and will determine its virulence. Scope Nematodes trigger crucial regulatory cytoprotective mechanisms, including antioxidant and detoxification pathways. Knowledge of the upstream regulatory components that contribute to both of these pathways in PPNs remains elusive. In this review, we discuss how PPNs probably orchestrate cytoprotection to resist plant immune responses, postulating that it may be derived from ancient molecular mechanisms. The review focuses on two transcription factors, DAF-16 and SKN-1, which are conserved in the animal kingdom and are central regulators of cell homeostasis and immune function. Both regulate the unfolding protein response and the antioxidant and detoxification pathways. DAF-16 and SKN-1 target a broad spectrum of Caenorhabditis elegans genes coding for numerous protein families present in the secretome of PPNs. Moreover, some regulatory elements of DAF-16 and SKN-1 from C. elegans have already been identified as important genes for PPN infection. Conclusion DAF-16 and SKN-1 genes may play a pivotal role in PPNs during parasitism. In the context of their hub status and mode of regulation, we suggest alternative strategies for control of PPNs through RNAi approaches.

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“…In agreement with www.nature.com/scientificreports www.nature.com/scientificreports/ this hypothesis, we have confirmed that downregulation of the MiDaf16-like and MiSkn1-like1 genes results in strong downregulation of genes from their networks during parasitism of M. incognita in single-or double-gene silencing plants. In this respect, the two superoxide dismutases (MiSod1-like1 and MiSod3-like1), peroxiredoxin (MiPRDX2-like1), glutathione S-transferase and peroxidase (MiGst1-like1 and MiGPX-like1), which act in the nematode antioxidant and detoxification pathways [18][19][20]63,64 , were indeed downregulated in M. incognita during parasitism in transgenic plants.…”

Section: Discussionmentioning

confidence: 96%

MiDaf16-like and MiSkn1-like gene families are reliable targets to develop biotechnological tools for the control and management of Meloidogyne incognita

Basso

Lourenço-Tessutti

Mendes

et al. 2020

Sci Rep

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Meloidogyne incognita is a plant-parasitic root-knot nematode (RKn, ppn) responsible for causing damage to several crops worldwide. in Caenorhabditis elegans, the DAF-16 and SKN-1 transcription factors (tfs) orchestrate aging, longevity, and defense responses to several stresses. Here, we report that MiDaf16-like1 and MiSkn1-like1, which are orthologous to DAF-16 and SKN-1 in C. elegans, and some of their targets, are modulated in M. incognita J2 during oxidative stress or plant parasitism. We used RnAi technology for the stable production of siRnAs in planta to downregulate the MiDaf16-like1 and MiSkn1-like1 genes of M. incognita during host plant parasitism. Arabidopsis thaliana and Nicotiana tabacum overexpressing a hairpin-derived dsRNA targeting these genes individually (single-gene silencing) or simultaneously (double-gene silencing) were generated. t 2 plants were challenged with M. incognita and the number of eggs, galls, and J2, and the nematode reproduction factor (NRF) were evaluated. our data indicate that MiDaf16-like1, MiSkn1-like1 and some genes from their networks are modulated in M. incognita J2 during oxidative stress or plant parasitism. Transgenic A. thaliana and N. tabacum plants with single-or double-gene silencing showed significant reductions in the numbers of eggs, J2, and galls, and in NRF. Additionally, the double-gene silencing plants had the highest resistance level. Gene expression assays confirmed the downregulation of the MiDaf16-like1 and MiSkn1-like1 tfs and defense genes in their networks during nematode parasitism in the transgenic plants. All these findings demonstrate that these two TFs are potential targets for the development of biotechnological tools for nematode control and management in economically important crops. Plant-parasitic nematodes (PPNs) are one of the major agricultural pathogens worldwide 1. PPNs disturb plant roots by altering the cell cycle, increasing the size of parasitized cells, and causing cell hyperproliferation. This process results in the compatible interaction and development of nematode feeding sites (galls) 2-6 , which disrupt the uptake of water and nutrients and reduce plant growth and yield 7-9. Root-knot nematodes (RKNs) are obligate sedentary endoparasites from the genus Meloidogyne spp. 10. Meloidogyne incognita is one of the most commonly reported species, causing damage in several crops of economic importance worldwide 11. Its life cycle comprises of six stages, namely, egg, J1 (first-stage juvenile), J2 (second-stage juvenile), J3 (third-stage juvenile), J4 (fourth-stage juvenile), and adults (female and male). The J3, J4, and females are typically sedentary endophytes, while the eggs and J2 are exophytes 11. The limited range of available control agents or resistant cultivars

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A peroxiredoxin,PRDX‐2, is required for insulin secretion and insulin/IIS‐dependent regulation of stress resistance and longevity

Cited by 58 publications

References 58 publications

Redox regulation of PGRL1 at the onset of low light intensity

Redox regulation of PGRL1 at the onset of low light intensity

Plant-parasitic nematodes: towards understanding molecular players in stress responses

MiDaf16-like and MiSkn1-like gene families are reliable targets to develop biotechnological tools for the control and management of Meloidogyne incognita

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