A PETase enzyme synthesised in the chloroplast of the microalga Chlamydomonas reinhardtii is active against post-consumer plastics

Abstract

Polyethylene terephthalate hydrolases (PETases) are a newly discovered and industrially important class of enzymes that catalyze the enzymatic degradation of polyethylene terephatalate (PET), one of the most abundant plastics in the world. The greater enzymatic efficiencies of PETases compared to close relatives from the cutinase and lipase families have resulted in increasing research interest. Despite this, further characterization of PETases is essential, particularly regarding their possible activity against other kinds of plastic. In this study, we exploited for the first time the use of the microalgal chloroplast for more sustainable synthesis of a PETase enzyme. A photosynthetic-restoration strategy was used to generate a marker-free transformant line of the green microalga Chlamydomonas reinhardtii in which the PETase from Ideonella sakaiensis was constitutively expressed in the chloroplast. Subsequently, the activity of the PETase against both PET and post-consumer plastics was investigated via atomic force microscopy, revealing evidence of degradation of the plastics.

The Microalgae <em>Chlamydomonas </em>for Bioremediation and Bioproduct Production

“…In Ideonella sakaiensis, a novel plastic degradation enzyme called PETase has been identified [96]. The I. sakaiensis PETase has been expressed through genetic recombination in the C. reinhardtii nucleus and chloroplast genomes, showing a significant ability to break down PET [97]. Under specific adverse conditions such as NaCl stress, EDTA exposure or acidic pH, C. reinhardtii can form multicellular aggregates called palmelloids.…”