A pH-sensitive gene delivery system based on folic acid-PEG-chitosan – PAMAM-plasmid DNA complexes for cancer cell targeting

The cellular uptake mechanism, intracellular transportation, and exocytosis of polyamidoamine dendrimers in multidrug-resistant breast cancer cells

“…For example, Yabbarov et al synthesized a three-component delivery system comprising a vector protein (recombinant receptor-binding fragment of Our laboratory has also conducted a number of studies on reversing MDR in cancer 12 and has prepared PAMAM/ HMGB1/pDNA nanocomplexes that can be used as high-efficiency gene carriers in vitro/vivo with high transfection and expression efficiency in sensitive breast cancer cells (MCF-7 cells). 13 We have further applied PAMAM/HMGB1/ pDNA nanocomplexes to multidrug-resistant breast cancer cells (MCF-7/ADR cells). Interestingly, we found the transfection and expression efficiency of PAMAM/HMGB1/pDNA nanocomplexes in MCF-7/ADR cells to be significantly lower than that in MCF-7 cells, which indicates that the transportation process of PAMAM-NH 2 dendrimers in drug-resistant tumor cells is different from that in tumor cells.…”

Folic Acid-Decorated Polyamidoamine Dendrimer Mediates Selective Uptake and High Expression of Genes in Head and Neck Cancer Cells

“…Therefore, it can be targeted for developing active tumor-targeting therapies [9][10][11]. Although FR-targeted drug and gene delivery systems have been developed on the basis of dendrimers [12][13][14][15][16][17] and many other types of nanoparticle carriers [18][19][20][21][22][23], few nonviral vectors have been developed for HNSCC [24,25]. Given the scarcity of nonviral vectors for gene therapy of HNSCC and limited clinical outcomes, it is highly desirable to develop and evaluate vectors under the context of HNSCC.…”

Novel guanidinylated bioresponsive poly(amidoamine)s designed for short hairpin RNA delivery

“…The mixtures were then incubated with 5 µL termination solution (400 mM NaCl, 100 mM ethylenediaminetetraacetic acid, pH 8) for 10 minutes at room temperature. 27 DNA integrity was assessed by agarose-gel electrophoresis after replacement with heparin solution (6 U/µL). The electrophoresis conditions were the same as described in the "Agarose gelretardation assay" section.…”

“…After the addition of 4 µL 6× loading buffer, 24 µL of the mixture was loaded onto a 1% agarose gel containing 0.5 µg/mL Goodview and electrophoresed. 27 The electrophoresis conditions were the same as described in the "Agarose gel-retardation assay" section.…”