A phage-initiated polysaccharide depolymerase in Pseudomonas putida

Transduction and the clustering of genes in fluorescent Pseudomonads.

“…PUG2 was found to lack all four activities and the cross PRS1 X PUG2 to cotransfer all four. Not on]y are the mandelate enzymes absent from fully induced PUG2 cells, but also CRM was not detected with antibodies directed against two of the enzymes purified from PRS1.8 1 A mutant of PRS1, lacking the mandelate dehydrogenase function (PRS3, mdlB1002) when crossed with PUG2 and transductants selected on benzoyl formate, gave cells containing the three other enzymes of the mandelate cluster (Table 7, columns 4 and 5). Thus we deduce that a rather large DNA fragment containing at least four genes functional in conversion of mandelate and benzoate is transferred from PRS1 -PUG2 in transduction.…”

Regulation of catabolic pathways in Pseudomonas.

“…Prospects for Pseudomonas genetics are bright (74). Systems have been developed for conjugation within P. aeruginosa (73,103,104,149) and P. putida (25), and for transduction within P. aeruginosa (76, 102) and P. putida (24,28,156). Contiguous structural genes have been identified, and linkage maps have been established in these species.…”

Genetics of Pseudomonas.

“…This has been demonstrated for both P. aeruginosa phage D3 (36) and P. putida phage pflS (23). Preliminary mapping of the phage chromosome has been done in each case, the mutants used being mainly plaque morphology mutants.…”