A phenanthrene-degrading strain Sphingomonas sp. GY2B isolated from contaminated soils

Tao, Xueqin; Lu, Guining; Dang, Zhi; Yang, Chen; Yi, Xiaoyun

doi:10.1016/j.procbio.2006.09.018

Cited by 130 publications

(65 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…5 mg/l (Table 2; Figure 14; Figure 15; Figure 16; Figure 17). This is consistent with a report by Tao et al (2007) who found that this bacterium has unique genes to degrade a wide range of PAH and related compounds. The gene is related from other Pseudomonas genera and reports so far in sequence homology and gene organization.…”

Section: Degradation Study Of Pahsupporting

confidence: 93%

Estimation of Bacterial Biodegradability of PAH in Khor Al-Zubair Channel, Southern Iraq

Alkanany¹,

Gmais²,

Maki³

et al. 2017

ijms

View full text Add to dashboard Cite

Four bacterial strains were isolated from Khor Al-Zubair channel, southern Iraq based on a high growth rate on crude oil and on hydrocarbon degradation ability. The strains were preliminarily identified based on morphological observation, and by the Vitek II system. The isolates were identified as Brevundimonas diminuta /vesicularis, Vibrio vulnificus, Sphingomonas paucimobilis and Ochrobactrum anthropic. However, the ability of these strains to utilize crude oil (0.25%; 0.5%; 1% and 2% v/v) varied both in rates of utilization and in growth profiles. The components of crude oil degraded by the isolates were determined by capillary gas chromatography. Sphingomonas paucimobilis and Vibrio vulnificus were the most effective bacteria degrade PAH on seventh day incubation, especially in concentration 2.0 mg/l (97.39% and 84.23%) respectively, meanwhile, Brevundimonas diminuta/ vesicularis was highly effective degrade PAH (73.41% a nd 62.08%) in concentration 1.0 and 0.5 mg/l, respectively. On the other hand, Ochrobactrum anthropi was the lowest in all concentrations.

show abstract

Section: Degradation Study Of Pahsupporting

confidence: 93%

Estimation of Bacterial Biodegradability of PAH in Khor Al-Zubair Channel, Southern Iraq

Alkanany¹,

Gmais²,

Maki³

et al. 2017

ijms

View full text Add to dashboard Cite

show abstract

“…The organic extracts were combined, dried with anhydrous Na 2 SO 4 , and evaporated at 35 C by a rotary evaporator (IKA, RV10, Germany). The extraction residues were dissolved in methanol (Tao et al, 2007). The metabolites of PHE degradation were analyzed by GCeMS (Agilent 7890A-5975C) and the separation column and column temperatures were as the same as for GC analysis.…”

Section: Methodsmentioning

confidence: 99%

“…(Tao et al, 2007). An enrichment technique was used to obtain the desired microbial consortia according to the previous literature (Ling et al, 2011;Masakorala et al, 2013).…”

Section: Isolation and Identification Of The Phe-degrading Strainmentioning

confidence: 99%

“…Colonies were harvested from dilution plates based on distinct colony morphology, and transferred to fresh PHE coated MSM agar plates several times to ensure culture purity. Single colony of each isolate was transferred to 10 mL of MSM solution, aliquots (1.8 mL) of liquid culture were used for DNA extraction using Ultra-clean microbial DNA isolation Kit (MoBio Laboratories, Carlsbad, CA) while 1 mL residual was cryopreserved at À80 C with 1 mL of 50% glycerol (Tao et al, 2007).…”

Section: Isolation and Identification Of The Phe-degrading Strainmentioning

confidence: 99%

“…As shown in Table 1, strain BZ-3 could use NA, ANTH, PYR, xylene, 1-hydroxy-2-naphthoic acid, and hexane as the sole carbon and energy source, but not nitrobenzene. It is worth noting that salicylic acid and catechol could be utilized by the strain BZ-3, which were considered as the intermediates in the PHE metabolism via salicylate route (Tao et al, 2007).…”

Section: Growth On Other Organic Compoundsmentioning

confidence: 99%

See 2 more Smart Citations

Biodegradation of phenanthrene by Pseudomonas sp. BZ-3, isolated from crude oil contaminated soil

Meng

Chen

et al. 2014

International Biodeterioration & Biodegradation

View full text Add to dashboard Cite

a b s t r a c tA phenanthrene (PHE) degrading bacterium strain BZ-3 was isolated from the crude oil contaminated soil in Binzhou, China. The isolate was identified as Pseudomonas sp. BZ-3 on the basis of 16S rRNA gene sequence. Various experiments were conducted to investigate the effect of pH, salinity and PHE concentration on the degradation efficiency of PHE. The degradation efficiency and degradation metabolites of PHE were detected by using GCeMS and HPLC-MS analyses. The strain BZ-3 could degrade 75% of PHE at an initial concentration of 50 mg/L under 20 g/L salinity in 7 days. PHE degradation kinetics was estimated in a first-order degradation rate model and the rate coefficient was calculated as 0.108 d À1. On the basis of the identified degradation metabolites, the strain BZ-3 could degrade PHE in the salicylate metabolic pathway. In a mixture system consisting of PHE and other PAHs including naphthalene (NA), anthracene (ANTH), and pyrene (PYR), the strain BZ-3 showed an efficiently degradation capability. Further study showed that the strain BZ-3 could also use NA, ANTH, PYR, xylene, 1-hydroxy-2-naphthoic acid, and hexane as the sole carbon and energy source, but did not grow on nitrobenzene-containing medium.

show abstract

In Situ Study on Biodegradation Differences Between Dissolved Phenanthrene and Methylphenanthrene Using First Derivative Synchronous Fluorescence Spectrometry With Double Scans

Li,

Chen,

Zhu

et al. 2024

Luminescence

View full text Add to dashboard Cite

A rapid and highly sensitive first derivative synchronous fluorescence spectrometry with double scans was successfully optimized for the simultaneous determination of dissolved phenanthrene (Phe), 1‐methylphenanthrene (1‐MP), and 3‐methylphenanthrene (3‐MP) and their metabolites such as 1‐hydroxy‐2‐naphthoic acid (1H2NA) and salicylic acid (SA) in the biodegradation processes of Phe, 1‐MP, and 3‐MP by Novosphingobium pentaromativorans US6‐1. Δλ of 55 and 109 nm were selected for Phe, 1‐MP, 3‐MP, 1H2NA, and SA, respectively. The intensities of the first derivative synchronous fluorescence detected at λex of 289, 292, 291, 354, and 312 nm for Phe, 1‐MP, 3‐MP, 1H2NA, and SA varied linearly with the concentrations of them in the ranges of 0.20 × 10−7–44.89 × 10−7, 0.20 × 10−7–13.04 × 10−7, 0.20 × 10−7–13.04 × 10−7, 0.22 × 10−7–76.30 × 10−7, and 0.11 × 10−7–52.00 × 10−7 mol/L. The limits of detection were 0.11 × 10−9, 0.30 × 10−9, 0.27 × 10−9, 0.33 × 10−9, and 2.90 × 10−9 mol/L for Phe, 1‐MP, 3‐MP, 1H2NA, and SA, respectively, with RSD less than 2%. The method was successfully applied in situ to detect the instantaneous concentrations of the PAHs and their metabolites, especially SA, during their biodegradation processes under simulated conditions in the lab. The results demonstrated that the upper metabolic pathways of 1‐MP and 3‐MP by US6‐1 were distinctly different from that of Phe owing to steric hindrance of the methyl group.

show abstract

A phenanthrene-degrading strain Sphingomonas sp. GY2B isolated from contaminated soils

Cited by 130 publications

References 26 publications

Estimation of Bacterial Biodegradability of PAH in Khor Al-Zubair Channel, Southern Iraq

Estimation of Bacterial Biodegradability of PAH in Khor Al-Zubair Channel, Southern Iraq

Biodegradation of phenanthrene by Pseudomonas sp. BZ-3, isolated from crude oil contaminated soil

In Situ Study on Biodegradation Differences Between Dissolved Phenanthrene and Methylphenanthrene Using First Derivative Synchronous Fluorescence Spectrometry With Double Scans

Contact Info

Product

Resources

About