A phenotype of IGFBP‐3 knockout mice revealed by dextran sulfate‐induced colitis

Yancu, Debbie; Blouin, Marie-José; Birman, Elena; Florianova, Livia; Aleynikova, Olga; Zakikhani, Mahvash; VanderMeulen, Heather; Seidman, Ernest G.; Pollak, Michael

doi:10.1111/jgh.13461

Cited by 7 publications

(6 citation statements)

References 25 publications

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“…IGFBP -3 knockout mice demonstrated a less active inflammatory state and lower levels of IL -6, TNF -α, and IL -1β, as well as increased proliferation of intestinal endothelial cells leading to repair of the endothelial barrier, which might confirm the role of the IGF axis in the development of the inflammatory state in patients with IBD. 30 In turn, an experimental study performed by Harris et al 31 in mice with colitis induced by dextran sodium sulfate showed a decrease in the level of plasma IGF -1; these results are concordant with our observations in adult patients with IBD. In conclusion, our results show that the IGF system is disrupted in patients with IBD.…”

Section: Patientssupporting

confidence: 81%

Insulin-like growth factor system in remission and flare of inflammatory bowel diseases

Krakowska-Stasiak¹,

Cibor²,

Domagała-Rodacka³

et al. 2017

Polish Archives of Internal Medicine

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Section: Patientssupporting

confidence: 81%

Insulin-like growth factor system in remission and flare of inflammatory bowel diseases

Krakowska-Stasiak¹,

Cibor²,

Domagała-Rodacka³

et al. 2017

Polish Archives of Internal Medicine

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“…On the contrary, metformin administration was effective in reducing number of tumors, the composition of resident inflammatory cells and other inflammatory scores, and the incidence of adenocarcinomas in the colon of AOM + DSS treated mice after 16-weeks. Similarly, the chemopreventive effect of metformin has been previously demonstrated [22] and the effects of IGFBP3 knockout and metformin were observed in a murine model of ulcerative colitis, showing significantly reduced colitis [30].…”

Section: Discussionmentioning

confidence: 78%

“…LYC was extracted from softgels and homogenized with NaCl 0.9% to provide the corresponding concentration; groups 6–7 (LYC 20 and LYC 50, n = 7) received 20 and 50 mg kg −1 of LYC, respectively. Metformin (1, 1-dimethylbiguanide hydrochloride) has been reported to provide in vivo and in vitro anti-inflammatory effects through inhibition of NF-κB signaling [22,30,44]; thus, a positive control group was included, and animals in group 8 (Metformin, n = 7) received 600 mg·kg −1 of metformin (Merck company, Naucalpan de Juárez, Edo de México, MEX), dissolved in NaCl 0.9%. The corresponding treatments were prepared daily and gavaged every morning (0.2 mL total volume) to all mice throughout the 16 weeks, with the exceptions of weeks 5 and 6.…”

Section: Methodsmentioning

confidence: 99%

Oral Administration of Microencapsulated B. Longum BAA-999 and Lycopene Modulates IGF-1/IGF-1R/IGFBP3 Protein Expressions in a Colorectal Murine Model

Valadez-Bustos

Escamilla‐Silva

García-Vázquez

et al. 2019

IJMS

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The Insulin-like growth factor-I/Insulin-like growth factor-I receptor (IGF-1/IGF-1R) system is a major determinant in colorectal cancer (CRC) pathogenesis. Probiotics (Bifidobacterium longum, BF) and lycopene (LYC) have been individually researched for their beneficial effects in the prevention of CRC. However, the effect of a combined treatment of microencapsulated BF and LYC on IGF-1/IGF-1R/IGFBPs (Insulin-like growth factor-binding proteins) expression in an azoxymethane (AOM)-dextran sulfate sodium (DSS)-induced CRC model have not been demonstrated. BF was microencapsulated by the spray drying technique, with high viability, and daily gavaged with LYC for 16 weeks to CD-1 mice in an AOM-DSS model. The results indicated that BF- and BF + LYC-treated groups had significantly lower inflammation grade, tumor incidence (13–38%) and adenocarcinoma (13–14%) incidence compared to the AOM + DSS group (80%), whereas LYC treatment only protected against inflammation grade and incidence. Caecal, colonic and fecal pH and β-glucuronidase (β-GA) values were significantly normalized by BF and LYC. Similarly, BF and BF + LYC treatments significantly reduced both the positive rate and expression grade of IGF-1 and IGF-1R proteins and normalized Insulin-like growth factor-binding protein-3 (IGFBP3) expression. Based on intestinal parameters related to the specific colon carcinogenesis in an AOM-DSS-induced model, LYC and microencapsulated BF supplementation resulted in a significant chemopreventive potential through the modulation of IGF-1/IGF-1R system.

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“…IGFBP-3 is a growth factor downregulating angiogenesis [ 52 ]. In addition, upregulation of IGFBP-3 is correlated with the improvement of colitic mice, and IGFBP-3 knockout animals are resistant to DSS-induced inflammation of the colon [ 53 , 54 ]. Interestingly, the level of IGFBP-3 in the intestine did not change under the influence of H. polygyrus in healthy mice.…”

Section: Discussionmentioning

confidence: 99%

Nematode-Induced Growth Factors Related to Angiogenesis in Autoimmune Disease Attenuation

Maruszewska-Cheruiyot

Krawczak-Wójcik

Michniowska

et al. 2023

Life

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Accumulating data suggest an important role of growth factors in autoimmune diseases and parasitic nematode infections. Nematodes are used in clinical studies of autoimmune diseases and parasite-derived molecules are widely studied for their therapeutic potential in various types of disorders. However, the effect of nematode infection on growth factors in autoimmune disorders has not been studied. The objective of this study was to evaluate the influence of infection with the intestinal nematode Heligmosomoides polygyrus in murine autoimmune models on the production of growth factors. Here, the level of a variety of growth factors related mainly to angiogenesis was evaluated by protein array in the intestinal mucosa of C57BL/6 dextran sodium sulfate-induced colitic mice and in cerebral spinal fluid of experimental autoimmune encephalomyelitis (EAE) mice infected with nematodes. In addition, vessel formation was evaluated in the brains of EAE mice infected with H. polygyrus. A significant influence of nematode infection on the level of angiogenic factors was observed. Parasitic infection of colitic mice resulted in upregulation of mucosal AREG, EGF, FGF-2, and IGFBP-3 in the intestine of the host and better adaptation (infectivity). In EAE mice, infection increased the level of FGF-2 and FGF-7 in CSF. In addition, remodeling of brain vessels was observed, with a higher density of long vessels. Nematode-derived factors are promising tools to fight autoimmune diseases and to study angiogenesis.

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A phenotype of IGFBP‐3 knockout mice revealed by dextran sulfate‐induced colitis

Abstract: Our data demonstrate that IGFBP-3 influences severity of DSS-induced colitis. The observations suggest that in the absence of IGFBP-3, enhanced IGF bioactivity leads to increased epithelial proliferation and mucosal barrier repair, thereby lessening inflammation.

Cited by 7 publications

References 25 publications

Insulin-like growth factor system in remission and flare of inflammatory bowel diseases

Insulin-like growth factor system in remission and flare of inflammatory bowel diseases

Oral Administration of Microencapsulated B. Longum BAA-999 and Lycopene Modulates IGF-1/IGF-1R/IGFBP3 Protein Expressions in a Colorectal Murine Model

Nematode-Induced Growth Factors Related to Angiogenesis in Autoimmune Disease Attenuation

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