A phenotyping strategy for evaluating the high-temperature tolerance of wheat

Thistlethwaite, Rebecca; Tan, Daniel K. Y.; Bokshi, A. I.; Ullah, Smi; Trethowan, Richard

doi:10.1016/j.fcr.2020.107905

Cited by 33 publications

(17 citation statements)

References 25 publications

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“…These included high-performing spring cultivars Suntop, Spitfire, Gregory, Janz, Hartog, EGA Wylie, Corack, Yitpi, Mace and Scout widely cultivated in major cropping regions of Australia. A set of eight CIMMYT genotypes described as heat tolerant under Australian environments was obtained from the University of Sydney (Thistlethwaite et al, 2020). Other genotypes used in these trials included donors of a multi-reference parent nested association mapping (MR-NAM) population developed for screening for heat and drought tolerance in wheat (Christopher et al, 2015(Christopher et al, , 2021Richard, 2017).…”

Section: Genotypesmentioning

confidence: 99%

“…Conventionally, wheat genotypes are screened for heat tolerance by serial sowings, using heat chambers in the field, or in controlled environments (e.g. Telfer et al, 2021Telfer et al, , 2018Thistlethwaite et al, 2020). Ranking for heat tolerance is typically based on physiological or morphological traits associated with plant function and performance (Bennett et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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A robust field-based method to screen heat tolerance in wheat

Ullah

Christopher

Frederiks³

et al. 2021

Preprint

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Wheat crops are highly sensitive to elevated temperatures, particularly during pollen meiosis and early grain filling. As the impact of heat stress greatly depends on the developmental stage of a crop, wheat germplasm ranking for heat tolerance in field experiments may be confounded by variation in developmental phase between genotypes at the time of heat events. Deploying an artificial-photoperiod-extension method (PEM) has allowed screening of diverse genotypes at matched developnemtal phases during natural heat events despite phenological varations. Irrigated experiments with 32 wheat genotypes were conducted in south-east Queensand, Australia with either (i) the PEM or (ii) conventional field plots. The Ppaired PEM and conventional field plot trials were sown at differentwith serial sowing dates from June to September.. In the PEM, plants were sown in single rows or in small plots and artificial supplemental lighting was installed at one end of each row/plot, extending day length to 20 h close to the lights. The intensity of supplementary lighting diminished as the distance from the lights increased, and induced a gradient of flowering times along each row/plot. Spikes of each genotype were tagged when they flowered. Late-sown crops received more heat shocks during early and/or mid-grainfilling than earlier sowings, and suffered significant yield losses. Significant genotypic differences in heat tolerance ranking were observed between PEM versus conventional plot screening. Individual grain weight of the tested wheat genotypes was strongy correlated in the PEM plots experiencing a similar degree of heat, but the correlation was either poor or negative in conventional plot trials. With PEM, we successfully quantified the impact post-flowering heat on individual grain weight of wheat genotypes with the heat events occurring precisely at a specific developmental stage. The PEM results produced robust field based rankings of genotypes for heat tolerance within trials experiencing similar heat events. This method promises to improve the efficiency of heat tolerance field screening, particularly when comparing genotypes of different maturity types.

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Section: Genotypesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A robust field-based method to screen heat tolerance in wheat

Ullah

Christopher

Frederiks³

et al. 2021

Preprint

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“…diurnal fluctuations in leaf sugar content) and a longer-term weeks-long scale (as evidenced by changes in T crit from heading to anthesis and grain filling). Anthesis is widely considered the stage of phenology at which wheat is most vulnerable to high temperature (Ferris et al ., 1998; Thistlethwaite et al ., 2020), and so it was somewhat surprising to see T crit rise as plants moved from heading to anthesis at the first glance. However, it is not surprising when considering the fact that anthesis vulnerability to heat stress is largely due to reduction of sink strength to import and utilize assimilates within the reproductive organs, rather than of assimilate supply from leaf photosynthesis per se (Li et al ., 2012; Ruan et al ., 2012).…”

Section: Discussionmentioning

confidence: 99%

Wheat photosystem II heat tolerance responds dynamically to short and long-term warming

Posch

Hammer

Atkin

et al. 2021

Preprint

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Heat-induced inhibition of photosynthesis is a key factor in declining wheat performance and yield. Variation in wheat heat tolerance can be characterised using the critical temperature (Tcrit) above which incipient damage to the photosynthetic machinery occurs. We investigated intraspecies variation and plasticity of wheat Tcrit under elevated temperature in field and controlled environment experiments. We also assessed whether intraspecies variation in wheat Tcrit mirrors patterns of global interspecies variation in heat tolerance reported for mostly wild, woody plants. In the field, wheat Tcrit varied through the course of a day, peaking at noon and lowest at sunrise, and increased as plants developed from heading to anthesis and grain filling. Under controlled temperature conditions, heat stress (36°C) was associated with a rapid rise in wheat Tcrit (i.e. within two hours of heat stress) that peaked after 3–4 days. These peaks in Tcrit indicate a physiological limitation to photosystem II heat tolerance. Analysis of a global dataset (comprising 183 Triticum and wild wheat (Aegilops) species) generated from the current study and a systematic literature review showed that wheat leaf Tcrit varied by up to 20°C (about two-thirds of reported global plant interspecies variation). However, unlike global patterns of interspecies Tcrit variation which has been linked to latitude of genotype origin, intraspecific variation in wheat Tcrit was unrelated to that. Yet, the observed genotypic variation and plasticity of wheat Tcrit suggests that this trait could be a useful tool for high-throughput phenotyping of wheat photosynthetic heat tolerance.

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“…At anthesis (Z61), controlled temperature chambers were placed over a 2 × 2 m area in two of the three plots (Supplementary Figure 2) to impose heat stress for 4 days. The chamber design and construction are described in detail by Thistlethwaite et al (2020). One of the chambers was maintained at ambient temperature (FCT1), and another heated between 10:00 and 16:00 to maintain +6 • C above ambient temperature (FCT2) within each replication per genotype.…”

Section: Field Experiments Using Potable Heat Chambersmentioning

confidence: 99%

The Physiological Basis of Improved Heat Tolerance in Selected Emmer-Derived Hexaploid Wheat Genotypes

2021

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Wheat is sensitive to high-temperature stress with crop development significantly impaired depending on the severity and timing of stress. Various physiological mechanisms have been identified as selection targets for heat tolerance; however, the complex nature of the trait and high genotype × temperature interaction limits the selection process. A three-tiered phenotyping strategy was used to overcome this limitation by using wheat genotypes developed from the ancient domesticated wheat, emmer (Triticum dicoccon Schrank), which was considered to have a wide variation for abiotic stress tolerance. A contrasting pair of emmer-based hexaploid lines (classified as tolerant; G1 and susceptible; G2) developed from a backcross to the same recurrent hexaploid parent was chosen based on heat stress responses in the field and was evaluated under controlled glasshouse conditions. The same pair of contrasting genotypes was also subsequently exposed to a short period of elevated temperature (4 days) at anthesis under field conditions using in-field temperature-controlled chambers. The glasshouse and field-based heat chambers produced comparable results. G1 was consistently better adapted to both extended and short periods of heat stress through slow leaf senescence under heat stress, which extended the grain filling period, increased photosynthetic capacity, increased grain filling rates, and resulted in greater kernel weight and higher yield. The use of a combination of phenotyping methods was effective in identifying heat tolerant materials and the mechanisms involved.

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A phenotyping strategy for evaluating the high-temperature tolerance of wheat

Cited by 33 publications

References 25 publications

A robust field-based method to screen heat tolerance in wheat

A robust field-based method to screen heat tolerance in wheat

Wheat photosystem II heat tolerance responds dynamically to short and long-term warming

The Physiological Basis of Improved Heat Tolerance in Selected Emmer-Derived Hexaploid Wheat Genotypes

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