A Philadelphia chromosome positive human leukaemia cell line (NALM-1) with pre-B characteristics

Minowada, Jun; Koshiba, Hirofumi; Jánossy, G.; Greaves, Melvyn F.; Bollum, F. J.

doi:10.1016/0145-2126(79)90050-x

Cited by 42 publications

(9 citation statements)

References 23 publications

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“…This cell line has been established from a patient with chronic myeloid leukemia in blast crisis [12]. It has a pre-B lymphoid phenotype [13]. After extensive absorption with tonsillar lymphocytes (2 x 1O9/ml) the antiserum still reacted mainly with C-ALL cells as tested by complement-dependent cytotoxicity (CDC) using "Cr-labeled target cells (Table 1).…”

Section: Antiseramentioning

confidence: 99%

Expression of HLA‐DR and common acute lymphoblastic leukemia antigens on glioma cells

Carrel¹,

Tribolet

Groß³

1982

Eur J Immunol

117

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The expression on several established human glioma cell lines of two well-defined differentiation antigens, HLA-DR and the common acute lymphoblastic leukemia antigen (CALLA) has been demonstrated. Rabbit anti-CALLA antiserum and monoclonal anti-Ia antibodies specifically lysed glioma cells in the presence of complement. Absorption of anti-CALLA antiserum and anti-Ia antibodies by glioma cells abolished their cytotoxicity against blasts isolated from a common acute lymphoblastic leukemia. Immunoprecipitation of solubilized glioma cells by monoclonal anti-Ia antibodies revealed two polypeptide chains of 28 and 33 kDa, whereas the anti-CALLA antiserum precipitated a single polypeptide chain of 100 kDa.

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Section: Antiseramentioning

confidence: 99%

Expression of HLA‐DR and common acute lymphoblastic leukemia antigens on glioma cells

Carrel¹,

Tribolet

Groß³

1982

Eur J Immunol

117

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“…REH [16], Nalm-1 [17], Nalm-6 [18], 380 [19], 697 [20], Ramos [21] and U-266 [22] cells were cultured in RPMI 1640 medium, whereas RS4;11 [23] was cultured in α-MEM medium (for details about the cell lines see Table S1). All culture media were supplemented with 10–20% fetal bovine serum, 100 µg/ml streptomycin and 100 IU/ml penicillin.…”

Section: Methodsmentioning

confidence: 99%

Proteomic Changes during B Cell Maturation: 2D-DIGE Approach

et al. 2013

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B cells play a pivotal role in adaptive immune system, since they maintain a delicate balance between recognition and clearance of foreign pathogens and tolerance to self. During maturation, B cells progress through a series of developmental stages defined by specific phenotypic surface markers and the rearrangement and expression of immunoglobulin (Ig) genes. To get insight into B cell proteome during the maturation pathway, we studied differential protein expression in eight human cell lines, which cover four distinctive developmental stages; early pre-B, pre-B, plasma cell and immature B cell upon anti-IgM stimulation. Our two-dimensional differential gel electrophoresis (2D-DIGE) and mass spectrometry based proteomic study indicates the involvement of large number of proteins with various functions. Notably, proteins related to cytoskeleton were relatively highly expressed in early pre-B and pre-B cells, whereas plasma cell proteome contained endoplasmic reticulum and Golgi system proteins. Our long time series analysis in anti-IgM stimulated Ramos B cells revealed the dynamic regulation of cytoskeleton organization, gene expression and metabolic pathways, among others. The findings are related to cellular processes in B cells and are discussed in relation to experimental information for the proteins and pathways they are involved in. Representative 2D-DIGE maps of different B cell maturation stages are available online at http://structure.bmc.lu.se/BcellProteome/.

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“…It is important to note that these patients had originally presented with typical Ph'-positive chronic granulocytic leukaemia (Minowada et al, 1979;Greaves and Vogler, personal communication). The simplest explanation for this intriguing clinical course is that in these patients the Ph' chromosome aberration had taken place in a common precursor of myeloid and phase B lymphoid cells which had a full maturation capacity to develop into myeloid cells and a limited maturation capacity to develop into pre-B lymphoid cells .…”

Section: Early Lymphoid Precursors In Human Bone Marrowmentioning

confidence: 99%

“…Furthermore, permanent cell lines of the non-T, non-B ALL phenotype (ALL+, TdT+, Ia+, cylgM-) and pre-B ALL phenotype (ALL+, TdT+, Ia+, cylgM+) exist and are available for large scale biochemical genetic studies (Minowada et al, , 1979Janossy et al, 1978; see also A. R. Williamson at page 76).…”

Section: Early Lymphoid Precursors In Human Bone Marrowmentioning

confidence: 99%