A Phosphofructokinase B-Type Carbohydrate Kinase Family Protein, NARA5, for Massive Expressions of Plastid-Encoded Photosynthetic Genes in Arabidopsis

Ogawa, Taro; Nishimura, Kenji; Aoki, Takehiko; Takase, Haruhiko; Tomizawa, Kenji; Ashida, Hiroshi; Yokota, Akiho

doi:10.1104/pp.109.139683

Cited by 22 publications

(25 citation statements)

References 79 publications

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“…In this study, we aimed to detect total mutations in the mutants without backcrossing. Despite this restriction, candidate mutations for those responsible for our focused mutant phenotypes ( Figure S1) were found in the mutated genes NARA5 (Ogawa et al, 2009) in Ar-57-al1; ASYMMET-RIC LEAVES1 (Byrne et al, 2000) in Ar-365-as1, and CYP85A2 (Kim et al, 2005) in Ar-443-as1. For forward genetics strategies in radiation-induced mutants identification of responsible genes will be made even more rapidly and efficiently by resequencing bulked segregants from a backcrossed population and detecting homozygous mutations in the genetic regions using the three algorithms.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

et al. 2015

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SUMMARYHeavy-ion beams are widely used for mutation breeding and molecular biology. Although the mutagenic effects of heavy-ion beam irradiation have been characterized by sequence analysis of some restricted chromosomal regions or loci, there have been no evaluations at the whole-genome level or of the detailed genomic rearrangements in the mutant genomes. In this study, using array comparative genomic hybridization (array-CGH) and resequencing, we comprehensively characterized the mutations in Arabidopsis thaliana genomes irradiated with Ar or Fe ions. We subsequently used this information to investigate the mutagenic effects of the heavy-ion beams. Array-CGH demonstrated that the average number of deleted areas per genome were 1.9 and 3.7 following Ar-ion and Fe-ion irradiation, respectively, with deletion sizes ranging from 149 to 602 180 bp; 81% of the deletions were accompanied by genomic rearrangements. To provide a further detailed analysis, the genomes of the mutants induced by Ar-ion beam irradiation were resequenced, and total mutations, including base substitutions, duplications, in/dels, inversions, and translocations, were detected using three algorithms. All three resequenced mutants had genomic rearrangements. Of the 22 DNA fragments that contributed to the rearrangements, 19 fragments were responsible for the intrachromosomal rearrangements, and multiple rearrangements were formed in the localized regions of the chromosomes. The interchromosomal rearrangements were detected in the multiply rearranged regions. These results indicate that the heavy-ion beams led to clustered DNA damage in the chromosome, and that they have great potential to induce complicated intrachromosomal rearrangements. Heavy-ion beams will prove useful as unique mutagens for plant breeding and the establishment of mutant lines.

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Section: Discussionmentioning

confidence: 99%

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

et al. 2015

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“…C. melo and Nicotiana tabacum (ecotype SR1) were grown under the same conditions, as for the wild watermelon, and fully-expanded fourth leaves were used for protein extraction. Arabidopsis thaliana (ecotype Col-0) was grown on Murashige and Skoog nutrient agar plates for 3 weeks, as described previously, 23) and the rosette leaves were used for protein extraction.…”

Section: Methodsmentioning

confidence: 99%

Potential Involvement of N-Terminal Acetylation in the Quantitative Regulation of the ε Subunit of Chloroplast ATP Synthase under Drought Stress

Hoshiyasu

Kohzuma

Yoshida

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…However, the function of s factors is likely to be redundant, and a specific one(s) for rbcL has not been reported. Protein factors such as CSP41 (Bollenbach et al, 2009) and NARA5 (Ogawa et al, 2009) have been reported to be involved in the transcription of rbcL via PEP in Arabidopsis, but they also affect the mRNA levels of genes for other chloroplast photosynthetic components. Thus, a pathway of specific transcriptional activation of rbcL has not yet been clarified.…”

Section: The Availability Of Rbcs Up-regulates Transcript Levels Of Rbclmentioning

confidence: 99%

Availability of Rubisco Small Subunit Up-Regulates the Transcript Levels of Large Subunit for Stoichiometric Assembly of Its Holoenzyme in Rice

Suzuki

Makino

2012

Plant Physiology

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Rubisco is composed of eight small subunits coded for by the nuclear RBCS multigene family and eight large subunits coded for by the rbcL gene in the plastome. For synthesis of the Rubisco holoenzyme, both genes need to be expressed coordinately. To investigate this molecular mechanism, the protein synthesis of two subunits of Rubisco was characterized in transgenic rice (Oryza sativa) plants with overexpression or antisense suppression of the RBCS gene. Total RBCS and rbcL messenger RNA (mRNA) levels and RBCS and RbcL synthesis simultaneously increased in RBCS-sense plants, although the increase in total RBCS mRNA level was greater. In RBCS-antisense plants, the levels of these mRNAs and the synthesis of the corresponding proteins declined to a similar extent. The amount of RBCS synthesized was tightly correlated with rbcL mRNA level among genotypes but not associated with changes in mRNA levels of other major chloroplast-encoded photosynthetic genes. The level of rbcL mRNA, in turn, was tightly correlated with the amount of RbcL synthesized, the molar ratio of RBCS synthesis to RbcL synthesis being identical irrespective of genotype. Polysome loading of rbcL mRNA was not changed. These results demonstrate that the availability of RBCS protein up-regulates the gene expression of rbcL primarily at the transcript level in a quantitative manner for stoichiometric assembly of Rubisco holoenzyme.

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A Phosphofructokinase B-Type Carbohydrate Kinase Family Protein, NARA5, for Massive Expressions of Plastid-Encoded Photosynthetic Genes in Arabidopsis

Cited by 22 publications

References 79 publications

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

Comprehensive identification of mutations induced by heavy‐ion beam irradiation in Arabidopsis thaliana

Potential Involvement of N-Terminal Acetylation in the Quantitative Regulation of the ε Subunit of Chloroplast ATP Synthase under Drought Stress

Availability of Rubisco Small Subunit Up-Regulates the Transcript Levels of Large Subunit for Stoichiometric Assembly of Its Holoenzyme in Rice

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