A photo-degradable supramolecular hydrogel for selective delivery of microRNA into 3D-cultured cells

Zhou, Zhengquan; Yi, Qikun; Xia, Tingting; Yin, Wencui; Kadi, Adnan A.; Li, Jinbo; Zhang, Yan

doi:10.1039/c6ob02667b

Cited by 16 publications

(11 citation statements)

References 50 publications

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“…In the area of "smart" materials, stimuli-responsive supramolecular peptide hydrogels, where gel disassembly can be triggered when required by means of a remote stimulus, are attracting attention owing to their potential biological applications in controlled drug release [18,19], conversion of self-assembling prodrugs [20][21][22], anti-microbial wound treatment [23,24], dissolution-on-demand wound dressings [25,26], and tunable cell culture platforms [27]. The non-invasive stimulus for gel dissolution can include treatment with UV light [28,29], the application of a magnetic field [30,31], the pH at the desired release site [32,33], or enzymatic degradation [34,35].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a Model Photo-Caged Dehydropeptide as a Stimuli-Responsive Supramolecular Hydrogel

et al. 2021

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Short peptides capped on the N-terminus with aromatic groups are often able to form supramolecular hydrogels, via self-assembly, in aqueous media. The rheological properties of these readily tunable hydrogels resemble those of the extracellular matrix (ECM) and therefore have potential for various biological applications, such as tissue engineering, biosensors, 3D bioprinting, drug delivery systems and wound dressings. We herein report a new photo-responsive supramolecular hydrogel based on a “caged” dehydropeptide (CNB-Phe-ΔPhe-OH 2), containing a photo-cleavable carboxy-2-nitrobenzyl (CNB) group. We have characterized this hydrogel using a range of techniques. Irradiation with UV light cleaves the pendant aromatic capping group, to liberate the corresponding uncaged model dehydropeptide (H-Phe-ΔPhe-OH 3), a process which was investigated by 1H NMR and HPLC studies. Crucially, this cleavage of the capping group is accompanied by dissolution of the hydrogel (studied visually and by fluorescence spectroscopy), as the delicate balance of intramolecular interactions within the hydrogel structure is disrupted. Hydrogels which can be disassembled non-invasively with temporal and spatial control have great potential for specialized on-demand drug release systems, wound dressing materials and various topical treatments. Both 2 and 3 were found to be non-cytotoxic to the human keratinocyte cell line, HaCaT. The UV-responsive hydrogel system reported here is complementary to previously reported related UV-responsive systems, which are generally composed of peptides formed from canonical amino acids, which are susceptible to enzymatic proteolysis in vivo. This system is based on a dehydrodipeptide structure which is known to confer proteolytic resistance. We have investigated the ability of the photo-activated system to accelerate the release of the antibiotic, ciprofloxacin, as well as some other small model drug compounds. We have also conducted some initial studies towards skin-related applications. Moreover, this model system could potentially be adapted for on-demand “self-delivery”, through the uncaging of known biologically active dehydrodipeptides.

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Section: Introductionmentioning

confidence: 99%

Evaluation of a Model Photo-Caged Dehydropeptide as a Stimuli-Responsive Supramolecular Hydrogel

et al. 2021

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“…24 This includes polymers that absorb light and undergo subsequent reversible thermally induced changes (and cargo release) to polymers that undergo irreversible bond cleavage and polymer dissolution. 25 (c) In the area of ''magnetic hydrogels'' numerous polymers have been designed 26 including magnetic hydrogel materials designed to be magnetically localized and those that are designed to undergo magnetically activated swelling, aggregation or degradation with the release of growth factors. 27 (d) The broad difference in pH between the stomach and other parts of the gastrointestinal (GI) tract allows pH-sensitive drug delivery systems to selectively deliver drugs.…”

Section: Introductionmentioning

confidence: 99%

Biodegradable pH-responsive hydrogels for controlled dual-drug release

Qiu

Yang

et al. 2018

J. Mater. Chem. B

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“…Anti-miR-221 Supramolecular hydrogels [68][69][70] miR-122 miR-122 mimics miRNA replacement miR-34a miR-34a mimics and subsequent down-regulation in the expression levels of its targets (∼80%). 68 Moreover, by using photosensitive molecules and targeting ligands as hydrogelator components, light-controlled and targeted delivery of miR-122 or miR-34a was also achieved.…”

Section: Carriersmentioning

confidence: 99%

MicroRNAs as novel endogenous targets for regulation and therapeutic treatments

Cha¹,

Fan²,

Zhou³

et al. 2018

Med. Chem. Commun.

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MicroRNAs (miRNAs) are small non-coding RNAs that have been identified as key endogenous biomolecules that are able to regulate gene expression at the post-transcriptional level. The abnormal expression or function of miRNAs has been demonstrated to be closely related to the occurrence or development of various human diseases, including cancers. Regulation of these abnormal miRNAs thus holds great promise for therapeutic treatments. In this review, we summarize exogenous molecules that are able to regulate endogenous miRNAs, including small molecule regulators of miRNAs and synthetic oligonucleotides. Strategies for screening small molecule regulators of miRNAs and recently reported small molecules are introduced and summarized. Synthetic oligonucleotides including antisense miRNA oligonucleotides and miRNA mimics, as well as delivery systems for these synthetic oligonucleotides to enter cells, that regulate endogenous miRNAs are also summarized. In addition, we discuss recent applications of these small molecules and synthetic oligonucleotides in therapeutic treatments. Overall, this review aims to provide a brief synopsis of recent achievements of using both small molecule regulators and synthetic oligonucleotides to regulate endogenous miRNAs and achieve therapeutic outcomes. We envision that these regulators of endogenous miRNAs will ultimately contribute to the development of new therapies in the future.

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A photo-degradable supramolecular hydrogel for selective delivery of microRNA into 3D-cultured cells

Cited by 16 publications

References 50 publications

Evaluation of a Model Photo-Caged Dehydropeptide as a Stimuli-Responsive Supramolecular Hydrogel

Evaluation of a Model Photo-Caged Dehydropeptide as a Stimuli-Responsive Supramolecular Hydrogel

Biodegradable pH-responsive hydrogels for controlled dual-drug release

MicroRNAs as novel endogenous targets for regulation and therapeutic treatments

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