A photoelectrochemical cell using immobilized photosynthetic membranes

Carpentier, Robert; Lemieux, Sylvie; Mimeault, Murielle; Purcell, Marc; Goetze, D. Christopher

doi:10.1016/0022-0728(89)87280-8

Cited by 10 publications

(8 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, the sensitivity toward the toxicants tested was similar (except for sodium dodecyl sulfate) in both the immobilized thylakoids and the thylakoid suspension even when the Chl concentration was more than 10 times greater for the immobilized thylakoids. This observation was reported before but comparative work had been done only with atrazine and diuron [17].…”

Section: Resultssupporting

confidence: 66%

“…As stressed before, with thylakoid suspensions, the sensitivity of the test improved by decreasing Chl concentration [17]. Interestingly, the sensitivity toward the toxicants tested was similar (except for sodium dodecyl sulfate) in both the immobilized thylakoids and the thylakoid suspension even when the Chl concentration was more than 10 times greater for the immobilized thylakoids.…”

Section: Resultsmentioning

confidence: 51%

“…The pattern of inhibition (i.e., highest at Chl at 250 μg/ml) was similar for the other light intensities investigated (data not shown). Previous studies with thylakoid membranes in suspension illustrated that the test was more sensitive to the presence of phytotoxic compounds at lower Chl concentrations because fewer inhibitor molecules were needed when fewer photosystems were present [17]. Although this explanation holds true for the slight increase in inhibition observed going from Chl at 300 μg/ml to 250 μg/ml with our immobilized samples, decreasing the concentration to 200 μg/ml or 150 μg/ml resulted in lower photocurrent inhibitions (Fig.…”

Section: Resultsmentioning

confidence: 66%

“…Previous work in our laboratory reported the use of Chl at 250 μg/ml in immobilized thylakoid membrane samples [17–19]. In the present study, some of the test conditions had been changed, and reassessing the optimal concentration was important.…”

Section: Resultsmentioning

confidence: 94%

See 3 more Smart Citations

In vitro phytotoxicity screening test using immobilized spinach thylakoids

Laberge

Chartrand

Rouillon

et al. 1999

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

Several pollutants found in water inhibit the photosynthetic electron transport chain, and therefore affect the growth of phytoplankton and aquatic plants. In this study, thylakoid membranes isolated from spinach leaves were used in a microelectro‐chemical cell to generate photocurrent. The toxic effect of an inhibitor is observed by a decrease in the photocurrent. To improve the stability of their biological functions, the thylakoid membranes were immobilized in an albumin–glutaraldehyde cross‐linked matrix. The developmental work of this phytotoxicity test was done by using the herbicide atrazine as the reference toxicant. Results on reproducibility were in the range generally accepted for standardized bioassays. The phytotoxicity of herbicides from various chemical classes including photosynthetic and nonphotosynthetic inhibitors was evaluated. Toxicity responses of the immobilized thylakoid test to photosynthetic inhibitors compared favorably with literature data for the algal growth inhibition test using Selen‐astrum capricornutum. The detection capabilities of the photosynthetic microassay for cyanazine, metribuzin, diuron, and propanil met the recommandation for the water quality guidelines for raw water. Characteristics of this in vitro approach such as rapidity, experimental simplicity, and cost effectiveness are also discussed.

show abstract

Section: Resultssupporting

confidence: 66%

Section: Resultsmentioning

confidence: 51%

Section: Resultsmentioning

confidence: 66%

Section: Resultsmentioning

confidence: 94%

See 2 more Smart Citations

In vitro phytotoxicity screening test using immobilized spinach thylakoids

Laberge

Chartrand

Rouillon

et al. 1999

Enviro Toxic and Chemistry

Self Cite

View full text Add to dashboard Cite

show abstract

“…The most common redox mediators used to extract electrons are quinones, which have been successfully employed with PSII-enriched membranes9, thylakoid membranes1011 or intact cells of cyanobacteria121314. Chloro-substituted benzoquinones were initially taken as effective electron acceptors of PSII151617.…”

mentioning

confidence: 99%

Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones

Picot

Choquet

et al. 2017

Nat Commun

View full text Add to dashboard Cite

Strategies to harness photosynthesis from living organisms to generate electrical power have long been considered, yet efficiency remains low. Here, we aimed to reroute photosynthetic electron flow in photosynthetic organisms without compromising their phototrophic properties. We show that 2,6-dimethyl-p-benzoquinone (DMBQ) can be used as an electron mediator to assess the efficiency of mutations designed to engineer a novel electron donation pathway downstream of the primary electron acceptor QA of Photosystem (PS) II in the green alga Chlamydomonas reinhardtii. Through the use of structural prediction studies and a screen of site-directed PSII mutants we show that modifying the environment of the QA site increases the reduction rate of DMBQ. Truncating the C-terminus of the PsbT subunit protruding in the stroma provides evidence that shortening the distance between QA and DMBQ leads to sustained electron transfer to DMBQ, as confirmed by chronoamperometry, consistent with a bypass of the natural QA°− to QB pathway.

show abstract

Photosynthetic Nanomaterial Hybrids for Bioelectricity and Renewable Energy Systems

et al. 2020

View full text Add to dashboard Cite

How PE harvesting systems have improved concerning solar energy absorption, PE production, and PE collection by electrodes is discussed. The review focuses on how different kinds of nanomaterials are applied and function in interfacing with photosynthetic materials for enhanced PE harvesting. Finally, the review analyzes how the performance of PE harvesting and stand-alone systems have evolved so far and its future prospects.

show abstract

A photoelectrochemical cell using immobilized photosynthetic membranes

Cited by 10 publications

References 18 publications

In vitro phytotoxicity screening test using immobilized spinach thylakoids

In vitro phytotoxicity screening test using immobilized spinach thylakoids

Redesigning the QA binding site of Photosystem II allows reduction of exogenous quinones

Photosynthetic Nanomaterial Hybrids for Bioelectricity and Renewable Energy Systems

Contact Info

Product

Resources

About