A Photographic Diagnostic Guide for Identification of the Principal Cranberry Fruit Rot Pathogens

Wells, Lindsay; McManus, Patricia S.

doi:10.1094/php-2013-0729-01-dg

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…vaccinii contribute to fruit rot diseases of cranberry in British Columbia. These pathogens have been previously reported to cause pre-and post-harvest fruit rot in other cranberry production regions in North America and elsewhere (Polashock et al, 2009;Wells and McManus, 2013;Polashock et al, 2017;Wells-Hansen and McManus, 2017;Conti et al, 2022). Based on a limited studies and anecdotal evidence, the fungal pathogens Allantophomopsis spp.…”

Section: Discussionmentioning

confidence: 99%

“…Isolates that failed to produce spores were exposed to 14 h UV-A (320-400 nm) and a 10 h dark photoperiod at 22°C to further induce sporulation (Leach, 1962;Dahlberg and Etten, 1982). Based on the colony characteristics and morphology of spores produced on culture media, fungal isolates were tentatively identified to their genus or, in some cases, species (Wells and McManus, 2013;Polashock et al, 2017;CMI Description of Pathogenic Fungi and Bacteria, 1969;Polashock et al, 2009). Based on the initial identification, a subset of representative isolates was further characterized using DNA-based molecular markers.…”

Section: Prevalence Incidence and Spatial Distributionmentioning

confidence: 99%

“…Phyllosticta vaccinii), Phomopsis vaccinii Shear (teleomorph Diaporthe vaccinii), and Botrytis spp. (Zuckerman, 1958;Stiles and Oudemans, 1999;Olatinwo et al, 2003;Polashock et al, 2009;Polashock et al, 2017;Wells-Hansen and McManus, 2017;Patten and Daniels, 2019;Conti et al, 2022). Although there is a general understanding of the fungal pathogens associated with cranberry fruit rot, their prevalence, incidence and spatial distribution in British Columbia have not been investigated previously.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and spatial distribution of cranberry fruit rot pathogens in British Columbia, Canada and potential fungicides for fruit rot management

Wood,

McBride,

Nabetani

et al. 2023

Front. Plant Sci.

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Twenty-eight cranberry farms in southwestern British Columbia were investigated for the prevalence and spatial distribution of fungal pathogens that contribute to fruit rot incidence. Farms were selected from six regions where most cranberry production is concentrated. Flowers, and green and ripe fruit (var. ‘Stevens’) samples, collected during two consecutive crop seasons, were analyzed for fruit rot pathogens. The most frequently isolated pathogens were identified as Allantophomopsis cytisporea, Botrytis cinerea, Coleophoma empetri, Colletotrichum fioriniae, Colletotrichum gloeosporioides, Fusicoccum putrefaciens, Glomerella sp., Phomopsis vaccinii, Phyllosticta elongata, Phyllosticta vaccinii and Physalospora vaccinii. The pathogens Allantophomopsis cytisporea, Phyllosticta spp., and Physalospora vaccinii were found at high incidence. These pathogens were present in all cranberry growing regions, although their mean percentage incidence varied from farm-to-farm and region-to-region. Amongst the pathogens from three phenological stages of cranberry crop examined, ripe fruit had the highest percentage incidence of fruit rot pathogens compared to that of flowers or green fruit; thus, indicating their presence at the early stages of crop development. The efficacy to inhibit the mycelial growth and spore germination of the fruit rot pathogens by twenty six fungicides, belonging to nine different modes of actions, were evaluated in vitro. The copper-based fungicides and captan of group M, flutriafol, triforine, difenoconazole, prothioconazole and propiconazole of group 3, benzovindiflupyr of group 7, and fosetyl-Al of group 33 demonstrated a high degree of efficacy in inhibiting the mycelial growth of all fruit rot pathogens. The fungicides chlorothalonil of group M, fenbuconazole of group 3, pyrimethanil and cyprodinil of group 9, and fludioxonil of group 12 also demonstrated activity against most fruit rot pathogens. The copper-based fungicides, chlorothalonil, captan, flutriafol, triforine, difenoconazole, prothioconazole, propiconazole, benzovindiflupyr, and fosetyl-Al effectively prevented the spore germination of most fruit rot pathogens. This demonstrated activity of the fungicides towards cranberry fruit rot pathogens should be assessed for efficacy in planta under field conditions. The current study identified the most critical fungal pathogens causing fruit rot of cranberry in British Columbia and potential fungicides that could be used in the management of fruit rot and to improve fruit quality and yield.

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Section: Discussionmentioning

confidence: 99%

Section: Prevalence Incidence and Spatial Distributionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and spatial distribution of cranberry fruit rot pathogens in British Columbia, Canada and potential fungicides for fruit rot management

Wood,

McBride,

Nabetani

et al. 2023

Front. Plant Sci.

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Long term crop rotation effect on subsequent soybean yield explained by soil and root-associated microbiomes and soil health indicators

Neupane

Bulbul

Wang

et al. 2021

Sci Rep

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Crop rotation is an important management tactic that farmers use to manage crop production and reduce pests and diseases. Long-term crop rotations may select groups of microbes that form beneficial or pathogenic associations with the following crops, which could explain observed crop yield differences with different crop sequences. To test this hypothesis, we used two locations each with four long-term (12–14-year), replicated, rotation treatments: continuous corn (CCC), corn/corn/soybean (SCC), corn/soybean (CSC), and soybean/corn (SCS). Afterwards, soybean was planted, and yield and soil health indicators, bulk soil microbiome, and soybean root-associated microbiome were assessed. Soybean yields, as well as soil protein, and POXC as soil health indicators were higher following CCC than in the other three treatments at both locations. A bacterial taxon in family JG30-KF-AS9 was enriched in CCC, whereas Microvirga, Rhodomicrobium, and Micromonosporaceae were enriched in SCS. Several ascomycetes explain lowered yield as soybean pathogens in SCS. Surprisingly, Tumularia, Pyrenochaetopsis and Schizothecium were enriched in soybean roots after CCC, suggesting corn pathogens colonizing soybean roots as nonpathogens. Our finding of associations between soil health indicators related to microbiomes and soybean yield has wide-ranging implications, opening the possibility of manipulating microbiomes to improve crop yield potential.

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A Molecular Assay Allows the Simultaneous Detection of 12 Fungi Causing Fruit Rot in Cranberry

Conti

Cinget

Vivancos³

et al. 2019

Plant Disease

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Cranberry fruit rot (CFR) is arguably one of the most limiting factors of cranberry (Vaccinium macrocarpon) production throughout its growing areas. The disease is caused by a group of closely related fungi that require identification using long and cumbersome steps of isolation and microscopic observations of structural features. The objective of this study was to develop a molecular assay to simultaneously detect and discriminate 12 of the most important fungal species reported to be pathogenic on cranberry fruit to facilitate the diagnosis of CFR. As the first approach, internal transcribed spacers and large subunit regions of all fungi were sequenced and confirmed with sequences available in the NCBI database. These data were used to develop primers able to differentiate seven of the 12 species. The five remaining species, including three in the Phacidiaceae family and two in the Glomerellaceae family, were differentiated on the basis of a more discriminant marker, the translation elongation factor 1-α. Two PCR reactions were optimized to clearly delineate the 12 species. The multiplex test was first validated using pure fungal cultures; it was subsequently validated using fruit collected in cranberry beds in eastern Canada. In the latter case, the test was rigorous enough to clearly discriminate the fungal pathogens from contaminants. Within the tested samples, Physalospora vaccinii and Coleophoma empetri were most commonly found. This molecular test offers scientists, diagnosticians, and growers a powerful tool that can rapidly and precisely identify fungi causing CFR so they can implement appropriate control methods.

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A Photographic Diagnostic Guide for Identification of the Principal Cranberry Fruit Rot Pathogens

Cited by 8 publications

References 9 publications

Prevalence and spatial distribution of cranberry fruit rot pathogens in British Columbia, Canada and potential fungicides for fruit rot management

Prevalence and spatial distribution of cranberry fruit rot pathogens in British Columbia, Canada and potential fungicides for fruit rot management

Long term crop rotation effect on subsequent soybean yield explained by soil and root-associated microbiomes and soil health indicators

A Molecular Assay Allows the Simultaneous Detection of 12 Fungi Causing Fruit Rot in Cranberry

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