A photoinduced growth system of peptide nanofibres addressed by DNA hybridization

Furutani, Masahiro; Uemura, Akihito; Shigenaga, Akira; Komiya, Chiaki; Otaka, Akira; Matsuura, Kazunori

doi:10.1039/c5cc01452b

Cited by 14 publications

(14 citation statements)

References 26 publications

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“…Conjugate 1 was synthesized according to our previous methods 49 . Conjugate 2 was synthesized by the same methods (Supplementary Scheme 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…We previously reported a light-induced peptide nanofibre growth system using conjugate 1 , consisting of β-sheet-forming FKFEFKFE peptide and addressing single-stranded DNA (dA 20 ), which are linked by a photo-cleavable amino acid X (Fig. 1b ) 49 . Ultraviolet (UV) irradiation to conjugate 1 releases free FKFEFKFE peptide, which self-assembles to form peptide nanofibre.…”

Section: Introductionmentioning

confidence: 99%

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Light-induced propulsion of a giant liposome driven by peptide nanofibre growth

Inaba

Uemura

Morishita

et al. 2018

Sci Rep

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Light-driven nano/micromotors are attracting much attention, not only as molecular devices but also as components of bioinspired robots. In nature, several pathogens such as Listeria use actin polymerisation machinery for their propulsion. Despite the development of various motors, it remains challenging to mimic natural systems to create artificial motors propelled by fibre formation. Herein, we report the propulsion of giant liposomes driven by light-induced peptide nanofibre growth on their surface. Peptide-DNA conjugates connected by a photocleavage unit were asymmetrically introduced onto phase-separated giant liposomes. Ultraviolet (UV) light irradiation cleaved the conjugates and released peptide units, which self-assembled into nanofibres, driving the translational movement of the liposomes. The velocity of the liposomes reflected the rates of the photocleavage reaction and subsequent fibre formation of the peptide-DNA conjugates. These results showed that chemical design of the light-induced peptide nanofibre formation is a useful approach to fabricating bioinspired motors with controllable motility.

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“…Conjugate 1 was synthesized according to our previous methods 49 . Conjugate 2 was synthesized by the same methods (Supplementary Scheme 1 ).…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Light-induced propulsion of a giant liposome driven by peptide nanofibre growth

Inaba

Uemura

Morishita

et al. 2018

Sci Rep

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“…Several Spr-containing peptides were already used to control function of peptides/ proteins in living cells. 14,16,21) Spr has been recently employed by some other groups for in-cell imaging of a protein of interest, 21) UV-induced propulsion of a giant liposome, 22) UV-triggered nanofiber formation, 23) and tumor-targeting therapy. 24) Application of Spr in topics not mentioned in this paper 14,17,18,25,26) are detailed in our recent review.…”

Section: Stimulus-responsive Amino Acidmentioning

confidence: 99%

Development of Chemical Biology Tools Focusing on Peptide/Amide Bond Cleavage Reaction

Shigenaga

2019

CHEMICAL & PHARMACEUTICAL BULLETIN

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Peptides and proteins are involved in almost all biological events. In this review, three chemical biology tools, which were developed for peptide/protein sciences from a viewpoint of peptide/amide bond cleavage, are overviewed. First, study on an artificial amino acid that enables stimulus-responsive functional control of peptides/proteins is briefly described. Two N-S acyl transfer reaction-based tools, one a linker molecule for facile identification of target proteins of bioactive compounds and the other a reagent for selective labeling of proteins of interest, are then discussed.

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“…Among the external stimuli, light is particularly attractive because of the ease of manipulation with localized and on‐demand irradiation. We developed a system of light‐induced peptide nanofiber growth with spatiotemporal control by using peptide‐DNA conjugates (Figure ) . We synthesized conjugate 1 consisting of a β‐sheet‐forming FKFEFKFE peptide and an addressing single‐stranded DNA (dA 20 ), which are linked by a photo‐cleavable amino acid X (Figure a).…”

Section: Self‐assembled Peptide Nanofibersmentioning

confidence: 99%

“…We developed a system of light-induced peptide nanofiber growth with spatiotemporal control by using peptide-DNA conjugates (Figure 8). [123] We synthesized conjugate 1 consisting of a b-sheet-forming FKFEFKFE peptide and an addressing single-stranded DNA (dA 20 ), which are linked by a photocleavable amino acid X (Figure 8a). Upon irradiation of UV light, conjugate 1 releases free FKFEFKFE peptide by the photocleavage reaction of X, which induces self-assembly of the peptide to form nanofibers 10-20 nm in width, as observed by TEM.…”

Section: Spatiotemporal Modulation Of Peptide Nanofiber Growthmentioning

confidence: 99%

Peptide Nanomaterials Designed from Natural Supramolecular Systems

Inaba

Matsuura

2018

The Chemical Record

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Natural supramolecular assemblies exhibit unique structural and functional properties that have been optimized over the course of evolution. Inspired by these natural systems, various bio‐nanomaterials have been developed using peptides, proteins, and nucleic acids as components. Peptides are attractive building blocks because they enable the important domains of natural protein assemblies to be isolated and optimized while retaining the original structures and functions. Furthermore, the peptide subunits can be conjugated with exogenous molecules such as peptides, proteins, nucleic acids, and metal nanoparticles to generate advanced functions. In this personal account, we summarize recent progress in the construction of peptide‐based nanomaterial designed from natural supramolecular systems, including (1) artificial viral capsids, (2) self‐assembled nanofibers, and (3) protein‐binding motifs. The peptides inspired by nature should provide new design principles for bio‐nanomaterials.

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A photoinduced growth system of peptide nanofibres addressed by DNA hybridization

Cited by 14 publications

References 26 publications

Light-induced propulsion of a giant liposome driven by peptide nanofibre growth

Light-induced propulsion of a giant liposome driven by peptide nanofibre growth

Development of Chemical Biology Tools Focusing on Peptide/Amide Bond Cleavage Reaction

Peptide Nanomaterials Designed from Natural Supramolecular Systems

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