A Photorhabdus akhurstii toxin altered gut homeostasis prior conferring cytotoxicity in Spodoptera frugiperda, S. litura and Helicoverpa armigera

Rao, Uma; Santhoshkumar, Kodhandaraman; Mathur, Chetna; Mandal, A.B.; Sagar, D.

doi:10.1007/s12600-021-00941-9

Cited by 8 publications

(8 citation statements)

References 48 publications

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“…These are typical characteristics of cellular degeneration in the midgut of insects fed on a bacterial toxin‐contaminated diet (Sousa et al, 2010). Similar histological changes in the midgut have been reported in S. frugiperda , S. litura and H. armigera larvae on exposure to Photorhabdus akhurstii, K. pneumonia and P. paralactis (Devi et al, 2022; Dutta et al, 2021). Thus, all these events may ultimately affect the nutritional parameters and fitness of the insect and delay the development period.…”

Section: Discussionsupporting

confidence: 73%

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Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura

Sarkhandia

Devi

Sharma

et al. 2023

J Applied Entomology

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Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) is a polyphagous pest causing economic losses to a number of agricultural crops. The present study was conducted to explore the insecticidal potential of some soil bacteria against S. litura. Among the tested bacterial isolates, Shewanella sp. (SS4) and Thauera sp. (M9) caused higher larval mortality. Concentration–response bioassays indicated a dose‐dependent increase in larval mortality with maximum mortality of 80 and 76%, respectively, due to Shewanella sp. and Thauera sp. Both the bacteria negatively affected the growth and development, reduced adult emergence and induced morphological deformities in adults. Nutritional parameters of S. litura i.e. relative growth and consumption rate, efficiency of conversion of ingested and digested food as well as approximate digestibility of food decreased with increasing bacterial concentrations. Histopathological studies revealed the disruption of peritrophic membrane and midgut epithelium of larvae. Profiles of digestive enzymes i.e. α‐amylases, α, β‐glucosidases and galactosidases, lipases and proteases were also significantly decreased with bacterial treatment. Comet assay test indicated DNA damage in larval haemocytes due to bacterial infection. These results imply that both these bacterial isolates have the potential as biological control agents and may prove as environmentally friendly alternative for pest management techniques.

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Section: Discussionsupporting

confidence: 73%

“…Finally, before mounting the samples in dibutylphthalate polysterene xylene (DPX), the slides were rinsed in xylene for 5 min. Images were then captured at 400× magnification using Evos XL core light microscope (Dutta et al, 2021).…”

Section: Methodsmentioning

confidence: 99%

Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura

Sarkhandia

Devi

Sharma

et al. 2023

J Applied Entomology

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“…Delta endotoxin and Vip3Aa toxin of B. thuringiensis have been known to damage the epithelial and muscle layers of the gut of lepidopteran insects [ 59 – 61 ]. Similar histological alterations in the midgut have been documented in S. frugiperda , S. litura , and H. armigera larvae following exposure to P. akhurstii , K. pneumoniae , and P. paralactis [ 37 , 43 ].…”

Section: Discussionsupporting

confidence: 53%

“…Similarly, other workers documented mortality in Spodoptera frugiperda (J.E. Smith), S. litura , Helicoverpa armigera (Hubner) due to the proliferation of Pseudomonas protogens and Photorhabdus akhurstii in hemocoel [ 36 , 37 ]. The presence of a high bacterial load in the hemolymph causes tissue necrosis as a result of bacterial toxins [ 38 ].…”

Section: Discussionmentioning

confidence: 96%

“…The slides were rinsed in 100, 90, 80 and 70% ethanol for 1 min each before being dewaxed in xylene for 10 min. The methodology by Dutta et al [ 37 ] was used to stain permanent slides with hematoxylin and eosin stain. Finally, before mounting the samples in Dibutylphthalate Polysterene Xylene (DPX), the slides were rinsed in xylene for 5 min.…”

Section: Methodsmentioning

confidence: 99%

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Larvicidal, growth inhibitory and biochemical effects of soil bacterium, Pseudomonas sp. EN4 against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae)

et al. 2023

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Background Spodoptera litura (Fabricius) (Lepidoptera: Noctuidae) also known as tobacco caterpillar, is one of the most serious polyphagous pests that cause economic losses to a variety of commercially important agricultural crops. Over the past few years, many conventional insecticides have been used to control this pest. However, the indiscriminate use of these chemicals has led to development of insecticide resistant populations of S. litura in addition to harmful effects on environment. Due to these ill effects, the emphasis is being laid on alternative eco-friendly control measures. Microbial control is one of the important components of integrated pest management. Thus, in search for novel biocontrol agents, the current work was carried out with the aim to evaluate the insecticidal potential of soil bacteria against S. litura. Results Among the tested soil bacterial isolates (EN1, EN2, AA5, EN4 and R1), maximum mortality (74%) was exhibited by Pseudomonas sp. (EN4). The larval mortality rate increased in a dose-dependent manner. Bacterial infection also significantly delayed the larval development, reduced adult emergence, and induced morphological deformities in adults of S. litura. Adverse effects were also detected on various nutritional parameters. The infected larvae showed a significant decrease in relative growth and consumption rate as well as efficiency of conversion of ingested and digested food to biomass. Histopathological studies indicated damage to the midgut epithelial layer of larvae due to the consumption of bacteria treated diet. The infected larvae also showed a significantly decreased level of various digestive enzymes. Furthermore, exposure to Pseudomonas sp. also caused DNA damage in the hemocytes of S. litura larvae. Conclusion Adverse effects of Pseudomonas sp. EN4 on various biological parameters of S. litura indicate that this soil bacterial strain may be used as an effective biocontrol agent against insect pests.

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Nanoemulsified formulation of cell-free supernatant from Photorhabdus luminescens as a sustainable biopesticide against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

Krithika,

Bellie,

Haran

et al. 2025

Crop Protection

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A Photorhabdus akhurstii toxin altered gut homeostasis prior conferring cytotoxicity in Spodoptera frugiperda, S. litura and Helicoverpa armigera

Cited by 8 publications

References 48 publications

Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura

Insecticidal, genotoxic and biochemical effects of Shewanella sp. (SS4) and Thauera sp. (M9) on Spodoptera litura

Larvicidal, growth inhibitory and biochemical effects of soil bacterium, Pseudomonas sp. EN4 against Spodoptera litura (Fab.) (Lepidoptera: Noctuidae)

Nanoemulsified formulation of cell-free supernatant from Photorhabdus luminescens as a sustainable biopesticide against Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae)

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