A photoswitchable GPCR-based opsin for presynaptic inhibition

Copits, Bryan A.; Gowrishankar, Raaj; O’Neill, Patrick; Li, Jun Nan; Girven, Kasey S.; Yoo, Judy; Meshik, Xenia; Parker, Kyle E.; Spangler, Skylar M.; Elerding, Abigail J.; Brown, Bobbie J.; Shirley, Sofia E.; K.L., Kelly; Vasquez, Alexis M.; Ständer, M; Kalyanaraman, Vani; Vogt, Sherri K.; Samineni, Vijay K.; Patriarchi, Tommaso; Tian, Lin; Gautam, N.; Sunahara, Roger K.; Gereau, Robert W.; Bruchas, Michael R.

doi:10.1016/j.neuron.2021.04.026

Cited by 77 publications

(80 citation statements)

References 128 publications

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“…Given these caveats, BiPOLES may not be suitable for bidirectional control of developing neurons or presynaptic boutons. In this case, silencing may be more efficient with rhodopsin pumps, despite their own limitations 37,49 or with G-protein coupled rhodopsins 50,51 . As with any optogenetic application, neurophysiological parameters need to be considered by the experimenter, guiding the appropriate choice of the tool suitable to address the specific experimental requirements.…”

Section: Discussionmentioning

confidence: 99%

BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons

et al. 2021

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Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control of neuronal activity allowing both excitation and inhibition of the same neurons in a single experiment is desired. This requires low spectral overlap between the excitatory and inhibitory opsin, matched photocurrent amplitudes and a fixed expression ratio. Moreover, independent activation of two distinct neuronal populations with different optogenetic actuators is still challenging due to blue-light sensitivity of all opsins. Here we report BiPOLES, an optogenetic tool for potent neuronal excitation and inhibition with light of two different wavelengths. BiPOLES enables sensitive, reliable dual-color neuronal spiking and silencing with single- or two-photon excitation, optical tuning of the membrane voltage, and independent optogenetic control of two neuronal populations using a second, blue-light sensitive opsin. The utility of BiPOLES is demonstrated in worms, flies, mice and ferrets.

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Section: Discussionmentioning

confidence: 99%

BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons

et al. 2021

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“…Axonal suppression can also be achieved using local chemogenetic approaches ( Stachniak et al, 2014 ); however, this method is not ideal if the temporal specificity of the suppression is critical. A new promising approach for axonal suppression is to inhibit synaptic vesicle release via an inhibitory G protein-coupled receptor ( Copits et al, 2021 ; Mahn et al, 2021 ).…”

Section: Advancements In Studying Cortical Computations By Long-range Connectivitymentioning

confidence: 99%

A Canonical Scheme of Bottom-Up and Top-Down Information Flows in the Frontoparietal Network

Hwang

Satō

Sato

2021

Front. Neural Circuits

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Goal-directed behavior often involves temporal separation and flexible context-dependent association between sensory input and motor output. The control of goal-directed behavior is proposed to lie in the frontoparietal network, but the computational architecture of this network remains elusive. Based on recent rodent studies that measured and manipulated projection neurons in the frontoparietal network together with findings from earlier primate studies, we propose a canonical scheme of information flows in this network. The parietofrontal pathway transmits the spatial information of a sensory stimulus or internal motor bias to drive motor programs in the frontal areas. This pathway might consist of multiple parallel connections, each controlling distinct motor effectors. The frontoparietal pathway sends the spatial information of cognitively processed motor plans through multiple parallel connections. Each of these connections could support distinct spatial functions that use the motor target information, including attention allocation, multi-body part coordination, and forward estimation of movement state (i.e., forward models). The parallel pathways in the frontoparietal network enable dynamic interactions between regions that are tuned for specific goal-directed behaviors. This scheme offers a promising framework within which the computational architecture of the frontoparietal network and the underlying circuit mechanisms can be delineated in a systematic way, providing a holistic understanding of information processing in this network. Clarifying this network may also improve the diagnosis and treatment of behavioral deficits associated with dysfunctional frontoparietal connectivity in various neurological disorders including Alzheimer’s disease.

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“…In particular, blue-emitting scintillators would be more desirable because many optogenetic tools are based on blue-sensing effector proteins (for reviews, see Repina et al, 2017;Goglia and Toettcher, 2019). Furthermore, UV-emitting Rb 2 CuBr 3 (Yang et al, 2019) is potentially useful for the activation of UV-sensing opsins such as parapinopsins (Koyanagi et al, 2004;Eickelbeck et al, 2020;Copits et al, 2021), OPN5 With technological advances, it might be possible to optically manipulate neurons in the human brain. This could aid the treatment of patients with various neurological disorders such as Parkinson's disease and epilepsy.…”

Section: Future Perspectives Toward Clinical Applications Of Optogeneticsmentioning

confidence: 99%

“…In addition to controlling the ionic conductance of the membrane, tools for the manipulation of intracellular signaling have also been developed. For example, photo-activatable G-protein-coupled receptors (e.g., Airan et al, 2009;Oh et al, 2010;Stierl et al, 2011;Karunarathne et al, 2013;Xu et al, 2014;Gao et al, 2015;Siuda et al, 2015;Copits et al, 2021;Mahn et al, 2021) (for a review, see Rost et al, 2017) can be used for slower modulation of the intracellular concentration of second messengers. Furthermore, development of various nonopsin based optogenetic systems has allowed for spatiotemporal regulation of protein functions, cellular signaling and gene expression (e.g., Wu et al, 2009b;Kennedy et al, 2010;Bugaj et al, 2013;Imayoshi et al, 2013;Konermann et al, 2013;Grusch et al, 2014;Wang et al, 2016;Zhou et al, 2017) (for reviews, see Repina et al, 2017;Goglia and Toettcher, 2019).…”

Section: Introductionmentioning

confidence: 99%

Remote Optogenetics Using Up/Down-Conversion Phosphors

Matsubara

Yamashita

2021

Front. Mol. Biosci.

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Microbial rhodopsins widely used for optogenetics are sensitive to light in the visible spectrum. As visible light is heavily scattered and absorbed by tissue, stimulating light for optogenetic control does not reach deep in the tissue irradiated from outside the subject body. Conventional optogenetics employs fiber optics inserted close to the target, which is highly invasive and poses various problems for researchers. Recent advances in material science integrated with neuroscience have enabled remote optogenetic control of neuronal activities in living animals using up- or down-conversion phosphors. The development of these methodologies has stimulated researchers to test novel strategies for less invasive, wireless control of cellular functions in the brain and other tissues. Here, we review recent reports related to these new technologies and discuss the current limitations and future perspectives toward the establishment of non-invasive optogenetics for clinical applications.

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A photoswitchable GPCR-based opsin for presynaptic inhibition

Cited by 77 publications

References 128 publications

BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons

BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons

A Canonical Scheme of Bottom-Up and Top-Down Information Flows in the Frontoparietal Network

Remote Optogenetics Using Up/Down-Conversion Phosphors

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