A phylogenetic analysis of Pseudonaja (Hydrophiinae, Elapidae, Serpentes) based on mitochondrial DNA sequences

Skinner, Adam; Donnellan, Stephen C.; Hutchinson, Mark N.; Hutchinson, Rhonda G.

doi:10.1016/j.ympev.2005.06.020

Cited by 26 publications

(56 citation statements)

References 36 publications

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“…One clade, the Pseudonaja nuchalis species (generically referred as the western brown snake), was recently split into three lineages based on their karyotypes, mitochondrial DNA sequences, and multivariate morphometric analysis [15][16].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…The Pseudonaja group has traditionally been problematic concerning their taxonomic classification at the species level [15]. Recently, the P. nuchalis species was split into 3 species (P. aspidorhyncha, P mengdeni and P. nuchalis) based on karyotypes, mitochondrial DNA sequences, and a multivariate morphometric analysis [15][16].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

“…Recently, the P. nuchalis species was split into 3 species (P. aspidorhyncha, P mengdeni and P. nuchalis) based on karyotypes, mitochondrial DNA sequences, and a multivariate morphometric analysis [15][16].…”

Section: Accepted M Manuscriptmentioning

confidence: 99%

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Deep venomics of the Pseudonaja genus reveals inter- and intra-specific variation

Reeks

Lavergne

Sunagar

et al. 2016

Journal of Proteomics

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Australian elapid venom remains an under-investigated resource of novel bioactive peptides. In this study, the venom gland transcriptomes and proteomes of the Australian western brown snakes, Pseudonaja aspidorhyncha and Pseudonaja nuchalis, were compared to Pseudonaja textilis. A deep venomics strategy incorporating high throughput 454 pyrosequencing gave a total of 200,911 raw reads for the three venoms. Subsequent annotation identified 5716 transcripts from 20 different toxin families with inter-specific variation between species observed in eight of the less abundant families. Integration of each venom proteome with the corresponding annotated reads identified 65 isoforms from six toxin families; high sequence coverage highlighted subtle differences between sequences and intra and inter-specific variation between species. High quality MS/MS data identified unusual glycoforms with natriuretic peptides from P. aspidorhyncha and P. nuchalis containing O-linked trisaccharides with high homology to the glycosylated region of TNPc. Molecular evolutionary assessments indicated the accelerated evolution of all toxin families with the exception of both natriuretic peptides and P. aspidorhyncha PLA2s that were found to be evolutionarily constrained under purifying selection pressures. This study has revealed a wide range of novel peptide sequences from six bioactive peptide families and highlights the subtle differences between toxins in these closely related species. Biological SignificanceMining Australia's vastly untapped source of toxins from its venomous creatures has been significantly advanced by employing deep venomics methodology.Technological advances in transcriptome analysis using next generation sequencing platforms and proteome analysis by highly sensitive tandem mass spectrometry allowed a more comprehensive interrogation of three underinvestigated brown snake (Pseudonaja) venoms uncovering many novel peptide sequences that are unique to these closely related species. This generic strategy will provide invaluable information when applied to other venomous snakes for a deeper understanding of venom composition, envenomation, venom evolution, as well as identifying research tools and drug leads.

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Section: Accepted M Manuscriptmentioning

confidence: 99%

Section: Accepted M Manuscriptmentioning

confidence: 99%

See 1 more Smart Citation

Deep venomics of the Pseudonaja genus reveals inter- and intra-specific variation

Reeks

Lavergne

Sunagar

et al. 2016

Journal of Proteomics

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“…Australian elapid systematics has advanced in recent years largely through phylogenetic analysis of new molecular and detailed morphological data (e.g., Smith 1982, Keogh 1998, 1999Keogh et al 1998Keogh et al ,2000Slowinski & Keogh 2000;Skinner et al 2005;Wüster et al 2005). Recently, subtly distinct, cryptic species have been described from widespread highly variable taxa (e.g., Keogh & Smith 1996;Aplin & Donnellan 1999).…”

Section: Introductionmentioning

confidence: 99%

“…The potential danger of O. scutellatus and to a lesser degree O. microlepidotus, has been well publicised (Worrell 1958;Stackhouse 1970;Jones 1977). Despite the high profile of taipans, and that the two species were described over 125 years ago, documentation of their ecology, behaviour, reproductive biology, relationships and distribution has been slow (Covacevich et al 1981;Covacevich 1987;Shine & Covacevich 1983;Skinner et al 2005). More recently, observations of captive taipans (Barnett 1978(Barnett , 1986 have complemented data on reproduction and feeding from available museum specimens (Shine and Covacevich 1983).…”

Section: Introductionmentioning

confidence: 99%

A new species of taipan (Elapidae: Oxyuranus) from central Australia

Doughty

Maryan

Donnellan

et al. 2007

Zootaxa

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Snakes in the Australo-Papuan elapid genus Oxyuranus are considered to be the most venomous species in the world. A recent expedition to the central ranges of Western Australia discovered a third species, which is described here from the only known specimen. Molecular genetic analyses using mitochondrial nucleotide sequences places the new species as the sister lineage of the two described Oxyuranus species, with all three species united by a long branch that also separates them from the nearest of the brown snakes species (Pseudonaja) to which the taipans are close relatives. Morphologically, the new species shares with the other Oxyuranus an undivided anal scale, high midbody scale row (21) and ventral scale (250) counts, but differs in having a single primary temporal scale and fewer lower labials (six). Maximum body size and venom potency are unknown. The discovery of a third species of taipan in the remote central ranges of Australia underlines the paucity of collecting from this region.

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Correlation between ontogenetic dietary shifts and venom variation in Australian brown snakes ( Pseudonaja )

Cipriani

Debono

Goldenberg

et al. 2017

Comparative Biochemistry and Physiology Part C: Toxicology &

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Venom is a key evolutionary trait, as evidenced by its widespread convergent evolution across the animal kingdom. In an escalating prey-predator arms race, venoms evolve rapidly to guarantee predatory or defensive success. Variation in venom composition is ubiquitous among snakes. Here, we tested variation in venom activity on substrates relevant to blood coagulation among Pseudonaja (brown snake) species, Australian elapids responsible for the majority of medically important human envenomations in Australia. A functional approach was employed to elucidate interspecific variation in venom activity in all nine currently recognised species of Pseudonaja. Fluorometric enzymatic activity assays were performed to test variation in whole venom procoagulant activity among species. Analyses confirmed the previously documented ontogenetic shift from non-coagulopathic venom in juveniles to coagulopathic venom as adults, except for the case of P. modesta, which retains non-coagulopathic venom as an adult. These shifts in venom activity correlate with documented ontogenetic shifts in diet among brown snakes from specialisation on reptilian prey as juveniles (and throughout the life cycle of P. modesta), to a more generalised diet in adults that includes mammals. The results of this study bring to light findings relevant to both clinical and evolutionary toxinology.

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A phylogenetic analysis of Pseudonaja (Hydrophiinae, Elapidae, Serpentes) based on mitochondrial DNA sequences

Cited by 26 publications

References 36 publications

Deep venomics of the Pseudonaja genus reveals inter- and intra-specific variation

Deep venomics of the Pseudonaja genus reveals inter- and intra-specific variation

A new species of taipan (Elapidae: Oxyuranus) from central Australia

Correlation between ontogenetic dietary shifts and venom variation in Australian brown snakes ( Pseudonaja )

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