A Phylogenic Analysis of Citrus Cultivars Native to
Jeju using Chloroplast DNA trnL-trnF and Internal
Transcribed Spacer Region Sequences

Jin, Seong Beom; Lee, Won Jeong; Park, Jae Ho; Park, Suk Man; Lee, Dong Hoon; Yun, Su Hyun

doi:10.12972/kjhst.20180059

Cited by 5 publications

(3 citation statements)

References 37 publications

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“…The genetic relationship between the donor (Lee) mandarin plants (control) and the anther culture-derived regenerated plants was analyzed as described previously by Jin et al (2016Jin et al ( , 2018Jin et al ( , 2022). The complete internal transcribed spacer (ITS) region of nuclear ribosomal DNA (ITS1-5.8S-ITS2) was analyzed using the primer pair ITS1F1 (5′-GAAGGATCATTGTCGACCTGCCAGCAGACG-3′) and ITS2R2…”

Section: Genetic Phylogenetic Analysismentioning

confidence: 99%

“…The ampli ed PCR products were electrophoresed on a 1.2% agarose gel at 100 V for 30 min and puri ed using a GeneAll® Gel Puri cation Kit (GeneAll Biotechnology, Co., Seoul, Korea). The puri ed products were cloned using the pLUG-Prime® TA-Cloning Vector Kit (iNtRON, Korea) and transformed into Escherichia coli (Jin et al 2018).…”

Section: Genetic Phylogenetic Analysismentioning

confidence: 99%

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Induction and genetic verification of homologous haploid plants obtained through the anther culture of Citrus cultivars

Jin,

Park,

Lee

et al. 2024

Preprint

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In this study, an anther culture system was developed for two Citrus varieties known for their genetic value: blood orange (Moro) and mandarin (Lee). Anthers were inoculated on N6 solid medium containing thidiazuron (TDZ, 0.44 mg/L), 6-benzylaminopurine (0.8 mg/L), zeatin (0.43 mg/L), kinetin (0.44 mg/L), 1-naphthaleneacetic acid (0.2 mg/L), 2,4-Dichlorophenoxyacetic acid (0.2 mg/L), and malt extract (500 mg/L). The inoculated anthers were treated with N6 liquid medium containing spermidine (200 µM) and gibberellic acid (GA3, 1 mg/L) and cultured for six weeks. Thereafter, the swollen anthers were transferred to Murashige and Skoog (MS) basal medium enriched with malt extract (500 mg/L), sucrose (50 g/L), TDZ (0.5 mg/L), GA3 (1 mg/L), and gelrite (0.2 %), which induced callus and somatic embryos. These somatic embryos from both varieties were then transferred to a germination medium (MS basal medium containing sorbitol [0.05 M], galactose [0.05 M], malt extract [500 mg/L], GA3 [0.5 mg/L], and gelrite [2 g/L]) to develop into normal plants. However, Lee exhibited significantly slower shoot and root growth compared to Moro. Genetic analysis using barley microsatellite-derived cleaved amplified polymorphic sequence markers indicated that Lee likely originated from haploid plants, whereas Moro retained heterozygosity similar to the parent. Ploidy analysis confirmed Lee as diploid, identical to the control. Internal transcribed spacer region analysis confirmed that Lee was an anther-cultured haploid-derived plant, estimated to be a homozygous diploid carrying recessive genes. These findings highlight potential applications in marker development and cultivar breeding enhancement focused on recessive trait-associated phenotypes and genotypes.

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Section: Genetic Phylogenetic Analysismentioning

confidence: 99%

Section: Genetic Phylogenetic Analysismentioning

confidence: 99%

Induction and genetic verification of homologous haploid plants obtained through the anther culture of Citrus cultivars

Jin,

Park,

Lee

et al. 2024

Preprint

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“…To select comparison markers, PCR products of approximately ≥700 bp, showing similar levels of amplification between 'Asumi' and 'Asuki', were excised. The excised PCR products were purified using the GgenAll ® Gel Purification Kit (GgenAll Biotechnology, Co., Seoul, Korea) and cloned using a pLUG-Prime ® TA-Cloning Vector Kit (iNtRON, Gyeonggi, Republic of Korea), as described by Jin et al [52]. The nucleotide sequences of the cloned PCR products were determined by Solgent Co., Ltd. (Daejeon, Korea).…”

Section: Cloning Of Pcr Amplification Productsmentioning

confidence: 99%

Development of Pollen Parent Cultivar-Specific SCAR Markers and a Multiplex SCAR-PCR System for Discrimination between Pollen Parent and Seed Parent in Citrus

Kim,

Han,

Park

et al. 2023

Plants

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This study discusses the challenge of distinguishing between two high-quality mandarin cultivars, ‘Asumi’ and ‘Asuki’, which have been introduced and cultivated in Korea after being developed through crossbreeding in Japan. Owing to genetic similarities resulting from crossbreeding between the same parent cultivars, it is challenging to differentiate them morphologically at the seedling stage. This difficulty poses challenges for cultivation and harvesting on farms. To address this issue, we developed a method using sequence characteristic amplification region (SCAR) markers for rapid and accurate differentiation between the two cultivars. We selected specific primer sets from random amplified polymorphic DNA–SCAR combinations and sequence-related amplified polymorphism contrast markers. The multiplex PCR system using these molecular markers was able to identify 16 mandarin cultivars, including ‘Asumi’ and ‘Asuki’, among 30 cultivars. The use of these SCAR markers is expected to enhance citrus cultivation by accurately identifying mixed cultivars and facilitating proper harvest timing for citrus distribution. Additionally, the markers can help identify the genetic traits of hybrid varieties at the seedling stage.

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Induction and genetic verification of homologous double haploid plants obtained through the anther culture of Citrus cultivars

Jin,

Park,

Lee

et al. 2024

Plant Cell Tiss Organ Cult

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A Phylogenic Analysis of Citrus Cultivars Native to Jeju using Chloroplast DNA trnL-trnF and Internal Transcribed Spacer Region Sequences

Abstract: This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cited by 5 publications

References 37 publications

Induction and genetic verification of homologous haploid plants obtained through the anther culture of Citrus cultivars

Induction and genetic verification of homologous haploid plants obtained through the anther culture of Citrus cultivars

Development of Pollen Parent Cultivar-Specific SCAR Markers and a Multiplex SCAR-PCR System for Discrimination between Pollen Parent and Seed Parent in Citrus

Induction and genetic verification of homologous double haploid plants obtained through the anther culture of Citrus cultivars

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