A physical map of the ribosomal DNA repeat unit of Aspergillus nidulans

Lockington, Robin; Taylor, Graham G.; Winther, Michael D.; Scazzocchio, Claudio; Davies, R. Wayne

doi:10.1016/0378-1119(82)90031-2

Cited by 44 publications

(6 citation statements)

References 12 publications

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“…The nuclear rDNA gene complex in filamentous fungi is normally 7.7-12 kb in length and codes for a single transcript containing the 18S, 5.8S, and 28S rRNA genes (Cox and Peden, 1979;Lockington et al, 1982;Labat et al, 1985). Sequence analysis of the entire V. dahliae rDNA unit reported in this study allows for useful conclusions to be drawn.…”

Section: Discussionmentioning

confidence: 94%

The Complete DNA Sequence of the Nuclear Ribosomal RNA Gene Complex of Verticillium dahliae: Intraspecific Heterogeneity within the Intergenic Spacer Region

Pramateftaki

Antoniou

Typas

2000

Fungal Genetics and Biology

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Section: Discussionmentioning

confidence: 94%

The Complete DNA Sequence of the Nuclear Ribosomal RNA Gene Complex of Verticillium dahliae: Intraspecific Heterogeneity within the Intergenic Spacer Region

Pramateftaki

Antoniou

Typas

2000

Fungal Genetics and Biology

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“…A. bisporus cDNAs. Data were normalized by hybridization to ribosomal DNA (pAR1 [21]) and to pBluescript. The results, shown as Phosphorlmager units, are the means ± standard deviations for three independent experiments.…”

Section: Cel3a W L S P F Y a D E V A Q A A A D I S N P S L A T K A A Smentioning

confidence: 99%

The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source

Chow

Yagüe

Raguz

et al. 1994

Appl Environ Microbiol

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A 52-kDa protein, CEL3, has been separated from the culture filtrate ofAgaricus bisporus during growth on cellulose. A PCR-derived probe was made, with a degenerate oligodeoxynucleotide derived from the amino acid sequence of a CEL3 CNBr cleavage product and was used to select ceI3 cDNA clones from an A. bisporus cDNA library. Two allelic cDNAs were isolated. They showed 98.8% identity of their nucleotide sequences. The deduced amino acid sequence and domain architecture of CEL3 showed a high degree of similarity to those of cellobiohydrolase II of Trichoderma reesei. Functional expression of cel3 cDNA in Saccharomyces cerevisiae was achieved by placing it under the control of a constitutive promoter and fusing it to the yeast invertase signal sequence. Recombinant CEL3 secreted by yeast showed enzymatic activity towards crystalline cellulose. At long reaction times, CEL3 was also able to degrade carboxymethyl cellulose. Northern (RNA) analysis showed that cel3 gene expression was induced by cellulose and repressed by glucose, fructose, 2-deoxyglucose, and lactose. Glycerol, mannitol, sorbitol, and maltose were neutral carbon sources. Nuclear run-on analysis showed that the rate of synthesis of cel3 mRNA in cellulose-grown cultures was 13 times higher than that in glucose-grown cultures. A low basal rate of cel3 mRNA synthesis was observed in the nuclei isolated from glucose-grown mycelia.

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“…In the first group, the 5S rDNA was located between the 28S and 18S of the rDNA repeat units, as e.g., in Armil-Brought to you by | New York University Bobst Library Technical Services Authenticated Download Date | 6/6/15 12:20 AM laria spp. In the second group, the 5S rDNA was dispersed elsewhere throughout the genome, as e.g., in Aspergillus nidulans (Lockington et al 1982). (Utomo et al 2005).…”

Section: Igsmentioning

confidence: 99%

Ribosomal DNA intergenic spacer of indoor wood-decay fungi

Schmidt

Moreth

2008

HFSG

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Indoor wood-decay fungi are economically very important. Approximately half of the total damage caused by indoor fungi in Germany is caused by species of the Coniophoraceae. The sequence of the intergenic spacer (IGS) region of the ribosomal DNA was elucidated with the following fungi of this family: Serpula lacrymans (true dry rot fungus), S. himantioides (wild merulius), Meruliporia incrassata (North American dry rot fungus), Leucogyrophana pinastri (mine dry rot fungus), Coniophora puteana (brown cellar fungus), and C. marmorata (marmoreus cellar fungus). The IGS length ranges between 2584 and 3785 bp and consists of the short IGS 1 (253-440 bp), the 5S rDNA (118 bp) and the long IGS 2 (2193-3310 bp). IGS 1 is phylogenetically less informative for the investigated Coniophoraceae species. 5S rDNA is transcribed in the reverse direction. IGS 2 contains extended repeat blocks of copies of different length. Intraspecific length polymorphism as a result of different copy number occurs in M. incrassata and L. pinastri. In combination with previous results, the fulllength sequence of the rDNA repeat unit is available for important indoor wood-decay fungi. The various rDNA regions can now be used for future identification of unknown sequences by BLAST and also for phylogenetic studies.

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A physical map of the ribosomal DNA repeat unit of Aspergillus nidulans

Cited by 44 publications

References 12 publications

The Complete DNA Sequence of the Nuclear Ribosomal RNA Gene Complex of Verticillium dahliae: Intraspecific Heterogeneity within the Intergenic Spacer Region

The Complete DNA Sequence of the Nuclear Ribosomal RNA Gene Complex of Verticillium dahliae: Intraspecific Heterogeneity within the Intergenic Spacer Region

The cel3 gene of Agaricus bisporus codes for a modular cellulase and is transcriptionally regulated by the carbon source

Ribosomal DNA intergenic spacer of indoor wood-decay fungi

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