A physiological characterization of the Cafeteria diet model of metabolic syndrome in the rat

Gomez-Smith, Mariana; Karthikeyan, Sudhir; Jeffers, Matthew S.; Janik, Rafal; Thomason, Lynsie A.M.; Stefanović, Bojana; Corbett, Dale

doi:10.1016/j.physbeh.2016.09.029

Cited by 79 publications

(83 citation statements)

References 63 publications

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“…Cafeteria diet induced an increase in body weight and body fat deposits, which replicates previous work (Gomes-Smith et al, 2016;Sagae et al, 2012Sagae et al, , 2013Sampey et al, 2011). Our results also indicate that early-life adversity did not differentially increase body weight or body fat deposits, despite the higher energy consumption by adversely reared animals fed cafeteria diet, a finding which contrasts with previously reported increases in adiposity in adversely reared mice exposed to a Western-style diet (Yam et al, 2017).…”

Section: Impact Of Early-life Adversity and Cafeteria Diet On Energysupporting

confidence: 86%

Metabolic dysfunction in a rat model of early‐life scarcity–adversity: Modulatory role of cafeteria diet

Sagae

Zanardini

Ribeiro-Paz

et al. 2018

Experimental Physiology

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Early-life adversity has become recognized as an important factor contributing to adult obesity and associated metabolic dysfunction. However, it is unclear whether obesity and metabolic dysfunction associated with early-life adversity result from coping strategies to deal with adversity-related emotional dysregulation, a direct programming of systems regulating metabolic function, or a combination. Interestingly, both early-life adversity and later-life dietary choices affect immune function, favouring pro-inflammatory mechanisms that are associated with obesity-related metabolic dysfunction. To investigate the unique and/or interactive effects of early-life adversity and later-life dietary choices for increased vulnerability to obesity and metabolic dysfunction, and specifically the role of the immune system in this vulnerability, we combined a naturalistic rat model of early-life scarcity-adversity with a rat model of obesity, the cafeteria diet. Our results indicate that early-life adversity alone induces insulin resistance, reduces pancreatic insulin secretion, plasma concentrations of triglycerides and cholesterol, and increases fasting glucose and tumour necrosis factor-α plasma concentrations. Importantly, animals exposed to adverse rearing were more vulnerable to metabolic dysregulation associated with the cafeteria diet, given that they consumed more energy, showed more severe hepatic steatosis and increased concentrations of the pro-inflammatory cytokine interleukin-1β than normally reared animals fed the cafeteria diet. Together, our results suggest that early-life adversity negatively programmes physiological systems that regulate metabolic function and increases vulnerability to obesity and metabolic dysfunction in adulthood. These results highlight the intrinsic relationship between the quality of the early postnatal environment and later-life dietary choices on adult health outcomes.

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Section: Impact Of Early-life Adversity and Cafeteria Diet On Energysupporting

confidence: 86%

Metabolic dysfunction in a rat model of early‐life scarcity–adversity: Modulatory role of cafeteria diet

Sagae

Zanardini

Ribeiro-Paz

et al. 2018

Experimental Physiology

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“…high-fat, high-sugar, high-salt, and low-fiber 19 . Frequently, laboratory´s rats are overweighed ad libitum-fed rodents, sedentary, obese and glucose intolerant.…”

Section: Acta Cir Bras 2017;32(3):251-262mentioning

confidence: 99%

Improving reproducibility and external validity. The role of standardization and data reporting of laboratory rat husbandry and housing

2017

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Purpose: To identify the most relevant flaws in standardization in husbandry practices and lack of transparency to report them. This review proposes some measures in order to improve transparency, reproducibility and eventually external validity in experimental surgery experiments with rat model. Methods:We performed a search of scientific articles in PUBMED data base. The survey was conducted from august 2016 to January 2017. The keywords used were "reproducibility", "external validity", "rat model", "rat husbandry", "rat housing", and the time frame was up to January 2017. Articles discarded were the ones which the abstract or the key words did not imply that the authors would discuss any relationship of husbandry and housing with the reproducibility and transparency of reporting animal experiment. Reviews and papers that discussed specifically reproducibility and data reporting transparency were laboriously explored, including references for other articles that could fulfil the inclusion criteria. A total of 246 articles were initially found but only 44 were selected. Results: Lack of transparency is the rule and not the exception when reporting results with rat model. This results in poor reproducibility and low external validity with the consequence of considerable loss of time and financial resources. There are still much to be done to improve compliance and adherence of researchers, editors and reviewers to adopt guidelines to mitigate some of the challenges that can impair reproducibility and external validity.Conclusions: Authors and reviewers should avoid pitfalls of absent, insufficient or inaccurate description of relevant information the rat model used. This information should be correctly published or reported on another source easily available for readers. Environmental conditions are well known by laboratory animal personnel and are well controlled in housing facilities, but usually neglected in experimental laboratories when the rat model is a novelty for the researcher.

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“…Exposure to the cafeteria-diet was also shown to induce behavioral changes -including reduced activity and low anxiety-like behavior-in rats [5]. Interestingly, it has also been shown that the withdrawal of CAF-diet results in partial recovery from some of the aforementioned metabolic and behavioral alterations associated with CAF-diet-intake [5,8].…”

Section: Introductionmentioning

confidence: 99%

“…Numerous recently published research works -focused at understanding the role of diet in inducing obesity and metabolic syndrome-have utilized rodent models of diet-induced obesity (DIO) through the administration of cafeteria (CAF) diets [1][2][3][4][5][6][7][8][9]. The CAF-diet consists of highly palatable, energy dense and unhealthy human junk food-items -with high-salt, high-sugar, high-fat and low-fiber content.…”

Section: Introductionmentioning

confidence: 99%

Comparing the metabolic fates of BALB/c mice maintained on cafeteria-style diets with differential nutritive values

Zaid

Ameer

Ali

et al. 2018

Preprint

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Cafeteria (CAF) diet-fed rodents are shown to provide a robust model of metabolic syndrome and human obesity. The carbohydrate/fat-rich food-items provided to the CAF-diet-model more closely approximate the ultra-processed human diet. However, most of the previous studies applied the commercially available rodent chow-diet for the comparative analyses and labeled it as a healthy-diet. The presented work aims to extend the knowledge on CAF-diet model by exposing the mice to human foods with different nutritional values. Our major goal was to study the metabolic fates of mice maintained on human food-items, which depending upon on their macronutrient compositions are categorized as healthy or unhealthy. BALB/c mice were randomly allocated to one of the three dietary intervention groups, standard chow diet; high-sugar/high-fat-cafeteria (HSHF-CAF) diet; or low-sugar/low-fat-cafeteria (LSLF-CAF) diet, for 5 weeks. The differences in multiple metabolic parameters (including food-/energy /macronutrient-intake, body-weight gain rate, organ-to-body weight ratios, plasma lipid profiles, adipocyte physiology, lipid deposition in metabolic tissues and ectopic fat storage in heart and kidney) were compared among the three intervention groups. We did not observe hyperphagia in mice maintained on CAF-diets. Nonetheless, the CAF-diet-fed mice displayed increased weight-gain-rate, adiposity, and adipocyte hypertrophy when compared to the chow-fed mice. However, the mice maintained on the two cafeteria-style diets displayed similar metabolic profiles, with HSHF-CAF-group displaying slightly higher weight-gain-rate. The HSHF-CAF-and LSLF-CAF-diet induced comparable adiposity in BALB/c mice. Further studies, with longer dietary intervention periods, are required to elucidate the effects of differential CAF-diets on the metabolic health of mice.

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A physiological characterization of the Cafeteria diet model of metabolic syndrome in the rat

Cited by 79 publications

References 63 publications

Metabolic dysfunction in a rat model of early‐life scarcity–adversity: Modulatory role of cafeteria diet

Metabolic dysfunction in a rat model of early‐life scarcity–adversity: Modulatory role of cafeteria diet

Improving reproducibility and external validity. The role of standardization and data reporting of laboratory rat husbandry and housing

Comparing the metabolic fates of BALB/c mice maintained on cafeteria-style diets with differential nutritive values

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