A Phytooxysterol, 28-Homobrassinolide Modulates Rat Testicular Steroidogenesis in Normal and Diabetic Rats

Abstract

Steroidogenesis in testicular cells depends upon the availability of cholesterol within testicular mitochondria besides the activities of 3b-hydroxysteroid dehydrogenase (3b-HSD, 17b-hydroxysteroid dehydrogenase [17b-HSD]), and the tissue levels of steroidogenic acute regulatory protein (StAR), androgen-binding protein (ABP), and testosterone (T). Cellular cholesterol biosynthesis is regulated by endogenous oxycholesterols acting through nuclear hormone receptors. Plant oxysterols, such as 28-homobrassinolide (28-HB), available to human through diet, was shown to exhibit antihyperglycemic effect in diabetic male rat. Its role in rat testicular steroidogenesis and lipid peroxidation (LPO) was therefore assessed using normal and streptozotocin-induced diabetic male rats. Administration of 28-HB (333 mg/kg body weight) by oral gavage for 15 consecutive days to experimental rats diminished LPO, increased antioxidant enzyme, 3b-HSD and 17b-HSD activities, and elevated StAR and ABP expression and T level in rat testis. We report that 28-HB induced steroidogenesis in normal and diabetic rat testis.

28-HOMOCASTASTERONE: A NOVEL DIETARY PHYTO KETO OXYSTEROL MODULATING TESTICULAR STEROID METABOLISM AND LxR mRNA EXPRESSION IN DIABETIC RAT

“…The observed sever decreases in testosterone level and steroidogenic enzyme activity in the diabetic animal considered as a consequent destruction of gonadic function due to hyperglycemia [14,15]. 28-HC greatly augmented rat testicular and plasma testosterone level both in treated normal and diabetic rats even though diabetic rat testicular testosterone level was greatly attenuated conforming that this brassinosteroid keto isoform functioned verse similar to the hydroxy form 28-HB in inducing testicular testosterone levels as reported by Premalatha et al [16].…”

Olive leaves extract attenuates type II diabetes mellitus-induced testicular damage in rats: Molecular and biochemical study

“…The present study was designed to elucidate the effects of olive extract on testicular steroidogenesis in STZ-induced diabetic rats. Accumulating evidences have verified that dysfunction of testicular steroidogenesis and spermatogenesis resulted from hyperglycaemia-induced oxidative stress and insulin deficiency is associated with male reproductive impairment (Premalatha et al, 2013).…”

Chitosan-Stabilized Selenium Nanoparticles and Metformin Synergistically Rescue Testicular Oxidative Damage and Steroidogenesis-Related Genes Dysregulation in High-Fat Diet/Streptozotocin-Induced Diabetic Rats

“…Accumulating evidence has confirmed that testicular steroidogenesis and spermatogenesis dysfunction correlate with male reproductive failure, due to hyperglycemic oxidative stress and insulin deficiency [ 84 ]. Testicular steroidogenesis is a highly regulated cholesterol-controlled signaling pathway that is dependent on cholesterol that is accessible within testicular mitochondria controlled by StAR [ 85 ].…”