A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on 1H NMR spectroscopy

Liu, Pengfei; Liu, Qinghui; Wu, Yin; Huang, Jie

doi:10.1016/j.jip.2014.09.008

Cited by 25 publications

(17 citation statements)

References 37 publications

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“…Another research indicates that glucose and lactate concentration increased in WSSV-infected shrimp at the stage of viral genome replication, and the glucose-6-phosphate dehydrogenase showed increased activity which is crucial enzyme of pentose phosphate pathway and lactate was shuttled into gluconeogenesis under normal biochemical situation. The decrease trend of lactate indicated that capacity of hydrogen had been changed and it contributed to the gluconeogenic supply of glucose for energy needing the infected shrimps (Solanky et al 2003;Chen et al 2011;Liu et al 2015). The time of group 2 shrimp which infected by WSSV were less than Fig.…”

Section: Discussionmentioning

confidence: 96%

A metabolic study in hepatopancreas of Litopenaeus vannamei response to white spot syndrome virus

Zhang

et al. 2017

Int Aquat Res

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White spot syndrome virus (WSSV) cause great harm in shrimp aquaculture. To understand the impact of viral infection on the shrimp metabolism, we monitored the culture farms of Litopenaeus vannamei and collected the samples on different stages of WSSV infection. The hepatopancreas of shrimp were separated, and then used gas chromatography mass spectrometry to detect the metabolites. Through the mass spectrometric analysis combined with multivariate data analysis, including PCA and OPLS models, metabolism of the shrimp was significantly changed by WSSV infection. The data indicated that in the early stage of WSSV infection, the glycolysis changed significantly, the contents of glucose and lactate increased distinctly. The metabolites of TCA cycle did not show up obviously regularity. The organism of fatty acids showed the same situation with glycolysis. At the early stage of infection, 14 amino acids metabolism were up-regulated, and glycine still increased at later stage of infection and the concentration was increased twice. The data of this study may provide some information to further research of viral disease mechanism.

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Section: Discussionmentioning

confidence: 96%

A metabolic study in hepatopancreas of Litopenaeus vannamei response to white spot syndrome virus

Zhang

et al. 2017

Int Aquat Res

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“…The ideal scenario involves the use of a training data set to build the model, and a separate validation data set to assess its predictive capacity. See Liu et al (2015) for an applied example of how PLS-DA and OPLS-DA were used to identify metabolites associated with white spot syndrome virus infection in shrimp, and provide preliminary information for developing biomarkers for diagnosing the pathophysiology of the disease.…”

Section: Multivariate Methodsmentioning

confidence: 99%

“…See Liu et al . () for an applied example of how PLS‐DA and OPLS‐DA were used to identify metabolites associated with white spot syndrome virus infection in shrimp, and provide preliminary information for developing biomarkers for diagnosing the pathophysiology of the disease.…”

Section: Metabolomic Strategiesmentioning

confidence: 99%

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Metabolomic strategies for aquaculture research: a primer

Young

Alfaro

2016

Reviews in Aquaculture

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Metabolomics is a fast-evolving field that provides qualitative and quantitative analyses of metabolites within cells, tissues or biofluids. Recent applications of metabolomics approaches in aquaculture research have highlighted the huge potential for solving problems within all aspects of the production line, from hatchery production to postharvest quality control. To assist with the growing application of metabolomics in aquaculture research, this contribution provides a review of techniques and steps necessary to conduct metabolomics research, from experimental design to data interpretation. Specifically, we target scientists who are new to the field of metabolomics, and we offer simple, but comprehensive steps and strategies to conduct this type of research. We conclude this primer with some advice on how to access relevant expertise and facilities for metabolomics-based aquaculture research.

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“…); to identify biomarkers involved in the host responses of shrimp to white spot syndrome virus (Liu et al . ); to characterize metabolic demands of hepatic tumours in flatfish (Southam et al . ); to discover metabolite biomarkers associated with enhanced host defence against bacterial infection in carp (Guo et al .…”

Section: Metabolomics Applications In Aquaculturementioning

confidence: 99%

Showcasing metabolomic applications in aquaculture: a review

Young

Alfaro

2016

Reviews in Aquaculture

132

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Aquaculture production is currently challenged to meet the growing demands for seafood protein throughout the world. To achieve this growth in an efficient, safe and sustainable manner, novel tools and applications will need to be incorporated at each step of the production line. A variety of 'omics' (e.g., transcriptomics, proteomics, metabolomics) applications have already begun to emerge in aquaculture research with extreme success. A promising new 'omics' approach is metabolomics, which aims to use metabolite profiles to identify biomarkers indicative of physiological responses of living samples (e.g., whole organism, tissues, cells) to environmental or culture conditions. One of the benefits of this approach is that it uses a broad scan of biological conditions to identify often unexpected problem or risk areas to focus management attention. In this contribution, we have selected relevant research examples to showcase the applications of metabolomics in aquaculture in four major areas: hatchery production, nutrition and diet, disease and immunology, and food safety and quality. The novelty of this approach is highlighted by the fact that we have cited the majority of published papers in this field, and these are all recent (within the last decade) contributions.

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A pilot metabolic profiling study in hepatopancreas of Litopenaeus vannamei with white spot syndrome virus based on 1H NMR spectroscopy

Cited by 25 publications

References 37 publications

A metabolic study in hepatopancreas of Litopenaeus vannamei response to white spot syndrome virus

A metabolic study in hepatopancreas of Litopenaeus vannamei response to white spot syndrome virus

Metabolomic strategies for aquaculture research: a primer

Showcasing metabolomic applications in aquaculture: a review

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