A pilot study on the DNA-protective, cytotoxic, and apoptosis-inducing properties of olive-leaf extracts

Anter, Jaouad; Fernández-Bedmar, Zahira; Villatoro-Pulido, M.; Demyda-Peyrás, S.; Moreno-Millán, M.; Alonso-Moraga, Ángeles; Muñoz-Serrano, A.; Castro, M.D. Luque de

doi:10.1016/j.mrgentox.2011.05.005

Cited by 70 publications

(56 citation statements)

References 57 publications

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“…In 2008, Fabiani et al (11) provided results suggesting that the complex mixture of phenols extracted from virgin olive and olive-mill wastewater reduced the DNA damage at concentrations of 1 µmol L -1 when coincubated in the medium with H 2 O 2 (40 µmol L -1 ). Another study (12) reported a dosedependent cytotoxic effect of olive leaf extract, obtained with an 80:20 ethanol/water mixture, on HL60 human promyelocytic leukemia cells with IC 50 of 10 µL mL -1 , indicating, by cell staining with acridine orange and ethidium bromide, the apoptotic pathway as the probable mechanism for the cytotoxic effect.…”

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confidence: 98%

“…The plant extract from olive leaves was reported to have anticancer, antioxidative and antinflammatory properties (12,13). In addition, the extract has demonstrated antimicrobial activity against pathogens (14), including the bacteria Salmonella typhi, Vibrio parahaemoliticus, Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeuroginosa, Bacillus subtilis (15)(16)(17).…”

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Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

et al. 2016

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Olive leaf extract activity against Candida albicans and C. dubliniensis -the in vitro viability studyOlive leaf extract is characterized by a high content of polyphenols (oleuropein, hydroxytyrosol and their derivatives), which is associated with its therapeutic properties. The objective of the present research was to evaluate the antifungal activity of olive leaf extract against Candida albicans ATCC 10231 and C. dubliniensis CBS 7987 strains. Minimum inhibitory concentrations (MIC) of the extract were determined by several in vitro assays. The extract showed a concentration depended effect on the viability of C. albicans with MIC value of 46.875 mg mL -1 and C. dubliniensis with MIC value 62.5 mg mL -1 . Most sensitive methods for testing the antifungal effect of the extracts were the trypan blue exclusion method and fluorescent dye exclusion method while MIC could not be determined by the method according to the EUCAST recommendation suggesting that herbal preparations contain compounds that may interfere with this susceptibility testing. The fluorescent dye exclusion method was also used for the assessment of morphological changes in the nuclei of treated cells. According to the obtained results, olive leaf extract is less effective against the tested strains than hydroxytyrosol, an olive plant constituent tested in our previous study.

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Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

et al. 2016

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“…Antigenotoxic agents generally exhibit desirable chemotherapeutic effects that could be efficient in the strategy of cancer control [51]. Hydrogen peroxide was used as a positive control in SMART because it is reported that it generates DNA damage through oxygen-radical mechanisms, gene mutation, chromosomal aberration and DNA single-strand breaks [52].…”

Section: Discussionmentioning

confidence: 99%

“…Anti-proliferative properties and induction of apoptosis in tumor cells are suitable when the health-protecting effect of antioxidants is assessed [51]. Apoptosis plays critical roles in the development and maintenance of homeostasis, in multicellular organisms.…”

Section: Discussionmentioning

confidence: 99%

Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Antigenotoxicity, Cytotoxicity and Apoptotic Effects

Martínez-Valdivieso¹,

Font²,

Fernández-Bedmar³

et al. 2017

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Zucchini (Cucurbita pepo subsp. pepo) is a seasonal vegetable with high nutritional and medical value. Many useful properties of this fruit are attributed to its bioactive compounds. Zucchini fruits ("Yellow" and "Light Green" varieties) and four distinctive components (lutein, β-carotene, zeaxanthin and dehydroascorbic acid)were selected. Firstly it was determinated the lutein, β-carotene, zeaxanthin and dehydroascorbic acid content in these fruits and then, in order to measure the safety and suitability of their use different assays were carried out: (i) Genotoxicity and antigenotoxicity tests to determine the safety and DNA-protection against hydrogen peroxide; (ii) cytotoxicity and (iii) DNA fragmentation assay to evaluate the proapoptotic effect. Results showed that: (i) all the substances were non-genotoxic, (ii) all the substances were antigenotoxic except the highest concentration of lutein, (iii) "Yellow" Zucchini epicarp and mesocarp exhibited the highest cytotoxic activity (IC50 > 0.1 mg/mL and 0.2 mg/mL, respectively) and iv) "Light Green" Zucchini skin and pulp induced internucleosomal DNA fragmentation . To sump up, Zucchini fruit could play a positive role in human health and nutrition due to this fruit and its components were safe, able to inhibit significantly the H2O2-induced damage and exhibit antiproliferative and proapoptotic properties toward HL60 tumour cells. The information generated from this research should take into account to select potential accessions for breeding programs purposes.

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“…The principal bioactive phenols in olive-leaf extracts (OLEs, rich in oleuropein and luteolin) in SMART test showed anti-genotoxic and non-significant mutagenic effects (Anter et al 2011) while schizonepetin, a naturally-occurring monoterpene at 0, 60, 120, and 240 mg/kg bw/day in mice and rats for 35 days after oral administration (p.o.) did not show genotoxic effects (Liu et al 2012).…”

Section: Monoterpenic Genotoxic/non-genotoxic Effectsmentioning

confidence: 99%

A review on genotoxic and mutagenic effects of monoterpenes

Islam

2017

IJM

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A pilot study on the DNA-protective, cytotoxic, and apoptosis-inducing properties of olive-leaf extracts

Cited by 70 publications

References 57 publications

Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

Olive leaf extract activity against Candida albicans and C. dubliniensis – the in vitro viability study

Role of Zucchini and Its Distinctive Components in the Modulation of Degenerative Processes: Genotoxicity, Antigenotoxicity, Cytotoxicity and Apoptotic Effects

A review on genotoxic and mutagenic effects of monoterpenes

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