A Pioneer Usage of Gold Nanoparticles in Sandwich Elisa Based on Circulating Hydatid Antigen as a Tool for Improvement of Serodiagnostic Testing

Rashed, Samia; Nasr, M E; Shalash, Ibrahim; Ali, Nagwa; EL-GHANNAM, AMIRA; Elawamy, Waleed E.

doi:10.21608/jesp.2020.131097

Cited by 1 publication

(1 citation statement)

References 22 publications

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“…25 Detection of circulating hydatid antigen in patients' serum samples by nanosilver sandwich ELISA This method followed that described in a previous study by Rashed et al, 2020. 26 Briefly, the wells of the microtiter plate were colored white with 100 μl of a 20 μg/ml of purified polyclonal IgG conjugated with AgNPs and diluted in 0.1 M carbonate buffer, pH 9.6 (Sigma, UK) overnight at room temperature, then washed 3 times. The steps were completed as in sandwich ELISA.…”

Section: Detection Of Circulating Hydatid Antigen In Human Sera Sampl...mentioning

confidence: 99%

Conjugated silver nanoparticles as a diagnostic tool for circulating hydatid antigens

Shakra

Abou-Sheishaa

Hafez

et al. 2022

EJI

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Cystic hydatid disease is one of the most significant worldwide zoonotic diseases. The causative agent is the larval stage of Echinococcus granulosus. The diagnosis of cystic echinococcosis by clinical symptoms and scanning alone is often difficult and confusing. The definite diagnosis needs sensitive and reliable serological tests. This study aimed to evaluate a nano silver-based enzyme linked immunosorbent assay (ELISA) in the detection of circulating hydatid antigen in human serum samples. The study included 66 human serum samples (36 hydatidosis confirmed cases, 15 cases infected with other parasites, and 15 normal subjects as negative control). The circulating protoscolices antigen was assayed by the nano-silver dot ELISA, nano-silver sandwich ELISA and the traditional methods (dot ELISA and sandwich ELISA). Our study revealed that the sensitivity and specificity of the nano-silver dot ELISA were 97.2% and 93.3%, respectively. The sensitivity and specificity of nano-silver sandwich ELISA were 94.4% and 96.7% respectively. The nano-silver-based ELISA showed higher sensitivity, specificity, positive predictive value, negative predictive value, and diagnostic accuracy than the traditional ELISA. In conclusion, the nano-silver-based ELISA can be proposed as a confirmatory test in the diagnosis of cystic echinococcosis.

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Section: Detection Of Circulating Hydatid Antigen In Human Sera Sampl...mentioning

confidence: 99%

Conjugated silver nanoparticles as a diagnostic tool for circulating hydatid antigens

Shakra

Abou-Sheishaa

Hafez

et al. 2022

EJI

View full text Add to dashboard Cite

show abstract

A Pioneer Usage of Gold Nanoparticles in Sandwich Elisa Based on Circulating Hydatid Antigen as a Tool for Improvement of Serodiagnostic Testing

Cited by 1 publication

References 22 publications

Conjugated silver nanoparticles as a diagnostic tool for circulating hydatid antigens

Conjugated silver nanoparticles as a diagnostic tool for circulating hydatid antigens

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