A pivotal peptide (Val-Ser-Glu-Glu) from duck egg white promotes calcium uptake and structure-activity relationship study

Hou, Tao; Lu, Yulin; Guo, Dayong; Liu, Weiwei; Shi, Wen; He, Hui

doi:10.1016/j.jff.2018.07.034

Cited by 15 publications

(11 citation statements)

References 39 publications

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“…Studies reported that many protein hydrolysates from different sources containing glutamic acids had calcium-chelating ability, such as soybean protein hydrolysates, porcine blood plasma protein hydrolysates, and wheat germ protein hydrolysates . With the gradual in-depth research in recent years, calcium-chelating peptides containing glutamic acid residues have been isolated and purified from different hydrolysates; their sequences were identified as Trp-Glu-Trp-Leu-His-Tyr-Trp (WEWLHYW) and Val-Ser-Glu-Glu (VSEE). , However, studies on the digestive process of peptides chelated with calcium are scarce, especially on the digestive stability and dynamic structural changes of the peptide–calcium complexes during gastrointestinal digestion.…”

Section: Introductionmentioning

confidence: 99%

“…12 With the gradual in-depth research in recent years, calcium-chelating peptides containing glutamic acid residues have been isolated and purified from different hydrolysates; their sequences were identified as Trp-Glu-Trp-Leu-His-Tyr-Trp (WEWLHYW) and Val-Ser-Glu-Glu (VSEE). 13,14 However, studies on the digestive process of peptides chelated with calcium are scarce, especially on the digestive stability and dynamic structural changes of the peptide−calcium complexes during gastrointestinal digestion.…”

Section: ■ Introductionmentioning

confidence: 99%

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Calcium Delivery Systems Assembled using Antarctic Krill Derived Heptapeptides: Exploration of the Assembly Mechanism, In Vitro Digestion Profile, and Calcium Absorption Behavior

Sun

Wang

et al. 2022

J. Agric. Food Chem.

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A novel heptapeptide QEELISK derived from Antarctic krill was used to assemble a calcium delivery system, of which the calcium binding mechanism of QEELISK, in vitro digestion kinetics, and calcium absorption behaviors were explored. QEELISK with continuous Glu possessed higher calcium binding capacity than that of QELEISK and QAALISK. Ca2+ bound to the carboxyl oxygen of Glu at position 3 of the QEELISK peptide at a stoichiometric ratio of 1:1 through charge–charge interaction; the formed QEELISK–Ca showed superior stability. Moreover, QEELISK–Ca underwent disaggregation and self-assembly during in vitro digestion reflected by visualization of calcium ions and circular dichroism spectra. QELEISK was partially stable during gastrointestinal digestion, and calcium chelation improved the digestive stability of QELEISK. In addition, a significant enhancement of calcium absorption with QELEISK–Ca occurred in the duodenum and ileum when compared to CaCl2 absorption, which indicated that QEELISK might carry calcium ions through the gastrointestinal tract.

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Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Calcium Delivery Systems Assembled using Antarctic Krill Derived Heptapeptides: Exploration of the Assembly Mechanism, In Vitro Digestion Profile, and Calcium Absorption Behavior

Sun

Wang

et al. 2022

J. Agric. Food Chem.

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“…The amount of soluble calcium in the proximal duodenum and jejunum determines the amount of calcium absorbed by the body. 6 However, absorption is influenced by dietary factors that form insoluble calcium salts, such as phytic acid, oxalate, and tannic acid, 7 as well as the alkaline pH in the intestine.…”

Section: ■ Introductionmentioning

confidence: 99%

Three Newly Isolated Calcium-Chelating Peptides from Tilapia Bone Collagen Hydrolysate Enhance Calcium Absorption Activity in Intestinal Caco-2 Cells

Liao

Chen

Jin

et al. 2020

J. Agric. Food Chem.

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In this study, we used reversed-phase high performance liquid chromatography (LC) to isolate three novel peptides with calcium-chelating capacity from tilapia bone collagen hydrolysate. Using LC–tandem mass spectrometry, we determined the amino acid sequences to be GPAGPHGPVG, FDHIVY, and YQEPVIAPKL. We then synthesized the three peptides and verified their calcium-chelating activity. Results showed that the calcium-chelating activity of GPAGPHGPVG, FDHIVY, and YQEPVIAPKL reached 18.80 ± 0.49, 35.73 ± 0.74, and 28.4 ± 0.94 mg/g, respectively. We next investigated how each peptide enhanced intestinal calcium absorption using Caco-2 cell monolayers. Compared with the control group, GPAGPHGPVG, FDHIVY, and YQEPVIAPKL potently enhanced calcium transport within 30 min by 89 ± 9, 202 ± 12, and 130 ± 7%, respectively. Results suggest that these peptides isolated from tilapia bone hydrolysate can be used as dietary supplements to increase calcium absorption.

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“…Caseinophosphopeptides (CPPs; Ser-Ser-Ser-Glu-Glu) can increase calcium absorption and were the first peptides found to have the ability to bind calcium ions [ 56 , 57 ]. Studies have reported that calcium-binding peptides derived from desalted duck egg white [ 58 ] and algae [ 59 ] also contain Ser. Another study found that the arginine carboxyl group of bradykinin is a metal-binding site [ 60 ].…”

Section: Resultsmentioning

confidence: 99%

Isolation, Purification and Structure Identification of a Calcium-Binding Peptide from Sheep Bone Protein Hydrolysate

Wang

Sun

et al. 2022

Foods

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To isolate a novel peptide with calcium-binding capacity, sheep bone protein was hydrolyzed sequentially using a dual-enzyme system (alcalase treatment following neutrase treatment) and investigated for its characteristics, separation, purification, and structure. The sheep bone protein hydrolysate (SBPH) was enriched in key amino acids such as Gly, Arg, Pro, Leu, Lys, Glu, Val, and Asp. The fluorescence spectra, circular dichroism spectra, and Fourier-transform infrared spectroscopy results showed that adding calcium ions decreased the α-helix and β-sheet content but significantly increased the random and β-turn content (p < 0.05). Carboxyl oxygen and amino nitrogen atoms of SBPH may participate in peptide–calcium binding. Scanning electron microscopy and energy dispersive spectrometry results showed that SBPH had strong calcium-chelating ability and that the peptide–calcium complex (SBPH–Ca) combined with calcium to form a spherical cluster structure. SBPH was separated and purified gradually by ultrafiltration, gel filtration chromatography, and reversed-phase high-performance liquid chromatography. Liquid chromatography-electrospray ionization/mass spectrometry identified the amino acid sequences as GPSGLPGERG (925.46 Da) and GAPGKDGVRG (912.48 Da), with calcium-binding capacities of 89.76 ± 0.19% and 88.26 ± 0.25%, respectively. The results of this study provide a scientific basis for the preparation of a new type of calcium supplement and high-value utilization of sheep bone.

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A pivotal peptide (Val-Ser-Glu-Glu) from duck egg white promotes calcium uptake and structure-activity relationship study

Cited by 15 publications

References 39 publications

Calcium Delivery Systems Assembled using Antarctic Krill Derived Heptapeptides: Exploration of the Assembly Mechanism, In Vitro Digestion Profile, and Calcium Absorption Behavior

Calcium Delivery Systems Assembled using Antarctic Krill Derived Heptapeptides: Exploration of the Assembly Mechanism, In Vitro Digestion Profile, and Calcium Absorption Behavior

Three Newly Isolated Calcium-Chelating Peptides from Tilapia Bone Collagen Hydrolysate Enhance Calcium Absorption Activity in Intestinal Caco-2 Cells

Isolation, Purification and Structure Identification of a Calcium-Binding Peptide from Sheep Bone Protein Hydrolysate

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