A Placebo-Controlled Treatment Trial of Blastocystis hominis Infection with Metronidazole

Nigro, Luciano; Larocca, Licia; Massarelli, Laura; Patamia, Ildebrando; Minniti, Salvatore; Palermo, Filippo; Cacopardo, Bruno

doi:10.2310/7060.2003.31714

Cited by 83 publications

(61 citation statements)

References 13 publications

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“…It was suggested that the intensity of infection should be reported, and the general criteria are whether five or more parasites are seen in a high-powered field (ϫ400) for wet mounts (77,133,166,188,194,215,319) or under oil immersion (ϫ1,000) (175, 179, 307) if permanent-stained smears are used. Using this criterion and in comparison with reports that do not use this criterion, accumulating studies suggest a correlation between infection density and symptoms (51,60,67,82,85,111,116,155,165,167,175,177,215,307). There are, however, a number of studies that reported a lack of such a correlation (63,86,131,145,180,216).…”

Section: Microscopymentioning

confidence: 99%

“…However, some infections persist for months if left untreated (90,94,112,131,165,175), and it is currently unknown if chronic infections influence seropositivity. A single study (112) of the serological response of Blastocystis-positive asymptomatic individuals showed that the highest IFA titer was obtained from a healthy individual infected with Blastocystis for 2 years.…”

Section: Serologymentioning

confidence: 99%

“…Symptoms can be variable, ranging from mild diarrheal illness (90) and chronic diarrhea (205) to acute gastroenteritis (16,136,161). A number of studies suggested that the finding of greater than five parasites per highpower field (ϫ400) or, less commonly, oil immersion (ϫ1,000) objective is associated with the acute presentation of gastrointestinal symptoms (51,67,82,85,111,116,155,165,167,177,215), while one study described an association between infection density and allergic cutaneous diseases (80). In a study of Blastocystis-positive patients from a Turkish hospital, the criteria for selection were the absence of any other coinfecting pathogens and the presence of more than five parasites per high-power field (116).…”

Section: Infection and Diseasementioning

confidence: 99%

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Extreme anoxia tolerance in embryos of the annual killifishAustrofundulus limnaeus: insights from a metabolomics analysis

Podrabsky

Lopez

Fan

et al. 2007

Journal of Experimental Biology

129

154

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SUMMARY The annual killifish Austrofundulus limnaeus survives in ephemeral pond habitats by producing drought-tolerant diapausing embryos. These embryos probably experience oxygen deprivation as part of their normal developmental environment. We assessed the anoxia tolerance of A. limnaeus embryos across the duration of embryonic development. Embryos develop a substantial tolerance to anoxia during early development, which peaks during diapause II. This extreme tolerance of anoxia is retained during the first 4 days of post-diapause II development and is then lost. Metabolism during anoxia appears to be supported mainly by production of lactate, with alanine and succinate production contributing to a lesser degree. Anoxic embryos also accumulate large quantities of γ-aminobutyrate (GABA), a potential protector of neural function. It appears that the suite of characters associated with normal development and entry into diapause II in this species prepares the embryos for long-term survival in anoxia even while the embryos are exposed to aerobic conditions. This is the first report of such extreme anoxia tolerance in a vertebrate embryo, and introduces a new model for the study of anoxia tolerance in vertebrates.

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Section: Microscopymentioning

confidence: 99%

Section: Serologymentioning

confidence: 99%

Section: Infection and Diseasementioning

confidence: 99%

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Extreme anoxia tolerance in embryos of the annual killifishAustrofundulus limnaeus: insights from a metabolomics analysis

Podrabsky

Lopez

Fan

et al. 2007

Journal of Experimental Biology

129

154

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show abstract

“…Los resultados se entregaron a los padres o acudientes de los menores y se suministró el tratamiento indicado en los casos con resultado positivo para Blastocystis sp. u otros parásitos intestinales patógenos (24).…”

Section: Consideraciones éTicasunclassified

Frecuencia y fuentes de Blastocystis sp. en niños urbanos de 0 a 5 años de hogares infantiles del estado del municipio de Calarcá, Colombia

et al. 2014

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“…Blastocystis spp. tespit edilen hastalarda ise metronidazol 750 mg/gün 7 gün kullanılması planlanmıştır (20,21). Sağaltım tamamlandıktan bir hafta sonra tüm hastalar kontrole çağrılmış, kısa bir görüşme yapılıp semptomların ortadan kalkıp kalkmadığı, verilen ilacın yan etkiye yol açıp açmadığı sorgulanmış, daha sonra aynı yöntemlerle dışkının parazitolojik incelemesi yapılmış ve parazitin eradike olup olmadığı araştırıl-mıştır.…”

Section: Introductionunclassified

İrrite Bağırsak Sendromunda Dientamoeba fragilis ve Blastocystis spp.'nin Rolü

Mumcuoğlu¹,

Coşkun²,

Aksu³

et al. 2013

TurkiyeParazitolDerg

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ÖZET Amaç: Bu çalışmada, irrite bağırsak sendromu (İBS) tanısı konulmuş hastalarda Dientamoeba fragilis ve Blastocystis spp. parazitlerinin sıklığı ve İBS oluşumuyla ilişkilerinin araştırılması amaçlanmıştır. Yöntemler: İBS tanısı almış 55 hastadan, akut gastroenterit şikayetleri olan 80 hastadan oluşan grubu 1'den (KG-1) ve sağlıklı 50 gönüllüden oluşan Kontrol Grubu 2'den (KG-2) toplanan dışkı örnekleri çalışmaya alınmıştır. Örnekler direkt mikroskobi, trikrom boyama ve spesifik kültür yapılarak incelenmiştir. Bulgular: Blastocystis spp. görülme sıklığı açısından İBS hasta grubu ile KG-1 arasında anlamlı fark görülmezken (p>0,05), KG-2 arasında anlamlı fark bulunmuştur (p<0,05). İBS hasta grubunda, direkt mikroskobide (X40) her sahada 5 ve üzeri Blastocystis spp. görülmesi kontrol gruplarından daha sıktır. Tedavi sonrası takiplerde 4 (%22,2) hasta şikayetlerinin tamamen geçtiğini bildirirken, 11 (%61,1) hasta diğer bulgularda hafifleme olmakla birlikte kabızlığın devam ettiğini, 3 (%16,7) hasta ise şikayetlerinde hiçbir değişiklik olmadığını bildirmiştir. Hiçbir örnekte D. fragilis tespit edilmemiştir. Sonuç: Hiçbir hastada D. fragilis tespit edilememiş olmasının nedeni bölgemizde bu parazitin infeksiyon oranının düşük olmasına bağlı olabilir. İBS hastalarında her mikroskobi sahasında (X40) 5 ve üzeri Blastocystis spp.'nin sık görülmesi ve bu hastaların önemli bir kısmında tedavi ile şikayetlerin gerilemesi, İBS ile Blastocystis spp. enfeksiyonları arasında bir bağlantı olabileceğini düşündürmüştür. (Turkiye Parazitol Derg 2013; 37: 73- Methods: Stool specimens collected from 55 IBS patients, 80 patients with gastroenteritis as control group 1 (CG-1) and 50 healthy volunteers as control group 2 (CG-2) were included the study. Samples were examined by direct microscopy, trichrome staining and culture methods. Results: While there was no significant difference in the prevalence of Blastocystis spp. between IBS patients and CG-1 (p>0.05), a significant difference was found between IBS and CG-2 (p<0.05). Patients with IBS were found to have five or more Blastocystis spp. per field than control groups. After eradication, all symptoms were cured in four patients, there were only constipation problems left in eleven patients and there were no changes in clinical findings in three patients. D. fragilis was not found in any of the samples. Conclusion:The reason we did not find any D. fragilis may be due to the low infection rate in the region. However, significantly having five or more Blastocystis spp. per field (X40) in IBS patients and regression of IBS symptoms after treatment in most of the patients suggested a possible link between IBS and Blastocystis spp. (Turkiye Parazitol Derg 2013; 37: 73-7)

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A Placebo-Controlled Treatment Trial of Blastocystis hominis Infection with Metronidazole

Cited by 83 publications

References 13 publications

Extreme anoxia tolerance in embryos of the annual killifishAustrofundulus limnaeus: insights from a metabolomics analysis

Extreme anoxia tolerance in embryos of the annual killifishAustrofundulus limnaeus: insights from a metabolomics analysis

Frecuencia y fuentes de Blastocystis sp. en niños urbanos de 0 a 5 años de hogares infantiles del estado del municipio de Calarcá, Colombia

İrrite Bağırsak Sendromunda Dientamoeba fragilis ve Blastocystis spp.'nin Rolü

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