A planar bilayer lipid membrane biosensor based on enzymes in the service of environmental monitoring

Abstract

The purpose of this study is the design of a biosensor taking into account the needs, the existing parameters and the environmental constraints in various ecosystems. Real time measurements from such biosensor could lead to reliable decisions about the status of an ecosystem. Two different approaches are presented in this paper. The first is a knowledge-based approach aimed to create an information tool suitable for measuring the critical parameters of a specific ecosystem. The second is an analytical development approach of the construction of a horseradish biosensor for peroxide detection.

Techno-economic design requirements for integrating biosensors in environmental monitoring of aquatic ecosystems

“…The results presented herein for the metal-supported enzyme biosensor built on horseradish peroxidase to quantitate peroxide and can be regarded as proof-of-principle for the detection of PAHs (Georgopoulos and Siontorou, 2016). Although the electrochemical study alone is not adequate to draw comprehensive conclusions, necessitating an in-depth investigation of the enzyme-lipid interactions and the mechanism of signal generation, quantitative detection has been demonstrated.…”

“…Sensor development is feasible using complicated and possibly multilayered interference suppression schemes. The monitoring of peroxide consumption is less prone to interference and has been investigated herein for the development of a metal-supported lipid-membrane biosensor with incorporated HRP for the indirect detection of PAHs (Georgopoulos and Siontorou, 2016). To our knowledge, such an approach has not been proposed to literature so far.…”