A planar interdigitated ring electrode array via dielectrophoresis for uniform patterning of cells

Manipulation of microparticles for construction of array patterns by negative dielectrophoresis using multilayered array and grid electrodes

“…DEP manipulation can be applied for various species, including non-charged materials, while electrophoretic manipulation is applied only for charged species. Therefore, many systems proposed in recent years have used DEP with a non-homogeneous electric field as the force for non-contact manipulation of cells or microparticles (Albrecht et al, 2006(Albrecht et al, , 2007Bocchi et al, 2009;Hsiung et al, 2008;Kim et al, 2007;Lee et al, 2008;Mittal et al, 2007;Peterson, 2005;Suzuki et al, 2004Suzuki et al, , 2007Suzuki et al, , 2008Taff and Voldman, 2005;Yantzi et al, 2007;Yasukawa et al, 2007). DEP techniques can be roughly categorized into three types, according to the way the electric field interacts with bioparticles; p-DEP, negative-DEP (n-DEP), and a combination of the two.…”

“…A method for the placement of microparticles or cells in arrays on a substrate is useful for studying and engineering interactions between cells (Albrecht et al, 2006;Ino et al, 2007Ino et al, , 2009), performing image-based cell selection (Taff and Voldman, 2005), creating cell-based biosensors, and high-throughput assays. Electrical forces, including electrophoresis and DEP, have been applied for the fabrication of microparticle and cell array patterns (Albrecht et al, 2006(Albrecht et al, , 2007Hsiung et al, 2008;Mittal et al, 2007;Taff and Voldman, 2005). DEP manipulation can be applied for various species, including non-charged materials, while electrophoretic manipulation is applied only for charged species.…”

“…The physical manipulation of biological particles is a vital component of miniaturized biotechnological platforms such as ''lab-on-a-chip'' devices and arrays for high-throughput assays. In recent years, many techniques regarding the manipulation of bioparticles on chips have been developed using hydrodynamics (El-Ali et al, 2006;Peterson 2005;Yang et al, 2007), magnetic forces (Ino et al, , 2009Ito et al, 2007Ito et al, , 2005Tsuchiya et al, 2008), dielectrophoresis (DEP) (Albrecht et al, 2006(Albrecht et al, , 2007Bocchi et al, 2009;Hsiung et al, 2008;Kim et al, 2007;Lee et al, 2008;Suzuki et al, 2004Suzuki et al, , 2007Suzuki et al, , 2008Taff and Voldman, 2005;Tornay et al, 2008;Wang et al, 2007;Yantzi et al, 2007;Yasukawa et al, 2007), electrophoresis , and pneumatic force (Hiranishi et al, 2007;Jeong and Konishi, 2009).…”

Label-free isolation of circulating tumor cells in microfluidic devices: Current research and perspectives

“…82 The main applications in biology are related to cell trapping, 83 focusing, 84 identification, 85 separation of cell populations, 86,87 or even cell patterning. [88][89][90] According to the electrical properties of the particles, medium and frequency of the electric field, the particles can move to high electric field strength (positive DEP) or to the low electric field strength (negative DEP). This aspect allows separating and collecting populations of cells in different locations of the microfluidic device.…”

Cell patterning using a dielectrophoretic–hydrodynamic trap

“…This study was further developed in (Albrecht et al 2006), reporting 3D micropatterning of chondrocytes within hydrogels using DEP and in (Albrecht et al 2007) where cells packaged in hydrogel microcapsules were trapped using pDEP generating a "tissue-like" structure. A uniform patterning of HepG2 cells in a 2D structure using a planar interdigitated ring electrode array is presented by Hsiung et al (2008). Ho et al (2006) demonstrate a 2D arrangement of cells in a structure that mimics the morphology of the liver tissue.…”