A plant-infecting subviral RNA associated with poleroviruses produces a subgenomic RNA which resists exonuclease XRN1 in vitro

Campbell, Alexandra J.; Anderson, John R.; Wilusz, Jeffrey

doi:10.1016/j.virol.2021.11.002

Cited by 4 publications

(2 citation statements)

References 57 publications

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“…In addition to the recently identified polerovirus, we detected contigs exhibiting homology with tombusvirus-like associated RNAs (tlaRNAs). These subviral, positivesense, single-stranded RNAs which are closely related to viruses of the family Tombusviridae, replicate autonomously but rely on a coinfecting virus for encapsidation and transmission 56,57 .…”

Section: Solemoviridae Family and Associated Virusesmentioning

confidence: 99%

Exploring the Viral Landscape of Saffron through Metatranscriptomic Analysis

Martinez-Fajardo,

Navarro-Simarro,

Morote

et al. 2024

Preprint

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Saffron (Crocus sativus L.), a historically significant crop valued for its nutraceutical properties, has been poorly explored from a phytosanitary perspective. This study conducted a thorough examination of viruses affecting saffron samples from Spanish cultivars, using high-throughput sequencing alongside a systematic survey of transcriptomic datasets from Crocus sativus at the Sequence Read Archive. Our analysis unveiled a broad diversity and abundance, identifying 17 viruses across the 45 analyzed libraries, some of which were highly prevalent. This includes known saffron-infecting viruses and previously unreported ones. In addition, we discovered novel viruses from the Alphaflexiviridae, Betaflexiviridae, Potyviridae, Solemoviridae, and Geminiviridae families, with some present in libraries from various locations. These findings indicate that the saffron-associated virome is more complex than previously reported, emphasizing the potential of phytosanitary analysis to enhance saffron productivity.

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Section: Solemoviridae Family and Associated Virusesmentioning

confidence: 99%

Exploring the Viral Landscape of Saffron through Metatranscriptomic Analysis

Martinez-Fajardo,

Navarro-Simarro,

Morote

et al. 2024

Preprint

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“…For example, the P0 protein of some poleroviruses, including TuYV, functions as a suppressor of the RNA interference (RNAi) system of the host plant (Baumberger et al, 2007;Bortolamiol et al, 2007;Csorba et al, 2010). TlaRNA ST9 was found to have a structural feature in the 3′ untranslated region (UTR) of its genomic RNA that functions to stall host XRN1 degradation (Campbell et al, 2022), which may explain the dramatic effect ST9 appeared to have on TuYV and CMoV accumulation in co-infected plants. The long distance movement protein (encoded by ORF3) of umbraviruses has been shown to form protective ribonucleoprotein complexes with both umbravirus and heterologous virus RNAs (Ryabov et al, 2001b;, and has also been shown in PEMV2 to protect (1979) showed that TuYV [formerly referred to as beet western yellows virus (BWYV)] was occasionally mechanically transmitted from plants co-infected with the umbravirus, lettuce speckles mottle virus (LSMV), and PLRV has been observed to gain mechanical transmissibility as a result of co-infection with PEMV2 (Falk et al, 1979;Ryabov et al, 2001a).…”

Section: Viruses In Inoculummentioning

confidence: 99%

Dissecting dynamic plant virus synergism in mixed infections of poleroviruses, umbraviruses, and tombusvirus-like associated RNAs

Erickson

Falk

2023

Front. Microbiol.

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Mixed infections of a plant infecting polerovirus, umbravirus, and/or tombusvirus-like associated RNAs (tlaRNAs) produce unique virus disease complexes that exemplify “helper-dependence” interactions, a type of viral synergism that occurs when a “dependent” virus that lacks genes encoding for certain protein products necessary for it to complete its infection cycle can utilize complementary proteins encoded by a co-infecting “helper” virus. While much research has focused on polerovirus-umbravirus or polerovirus-tlaRNA interactions, only recently have umbravirus-tlaRNA interactions begun to be explored. To expand on the limited understanding of umbravirus-tlaRNA interactions in such disease complexes, we established various co-infection pairings of the polerovirus turnip yellows virus (TuYV), the umbravirus carrot mottle virus (CMoV), and three different tlaRNAs—carrot red leaf virus aRNAs (CRLVaRNAs) gamma and sigma, and the TuYVaRNA ST9—in the model plant Nicotiana benthamiana, then investigated the effects of these different co-infections on tlaRNA systemic movement within the host, and on virus accumulation, and aphid and mechanical transmission of each of these viruses. We found that CMoV alone could support systemic movement of each of the tlaRNAs, making this the second report to demonstrate such an interaction between an umbravirus and tlaRNAs. We also report for the first time that CMoV could also impart mechanical transmissibility to the tlaRNAs sigma and ST9, and that co-infections of either of these tlaRNAs with both TuYV and CMoV increased the efficiency with which TuYV could be mechanically co-transmitted with CMoV.

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