A Plasma Biochemical Analysis of Acute Lead Poisoning in a Rat Model by Chemometrics-Based Fourier Transform Infrared Spectroscopy: An Exploratory Study

Tian, Wenli; Wang, Dan; Fan, Haoran; Yang, Lüjuan; Ma, Gang

doi:10.3389/fchem.2018.00261

Cited by 13 publications

(6 citation statements)

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“…[ 10 ] As reported by previous studies, elevated blood lead concentration is associated with hematologic findings such as BS of red cells and hemolytic anemia, and it has been proven that decreased P5N activity is partially responsible for these findings. [ 10 11 ] Also, high blood lead level (BLL) could damage the majority of soft tissues mainly the liver. [ 10 ] The central role of hepatic tissue in detoxification emphasizes the importance of liver function in clearance of xenobiotics especially in drug-addicted individuals.…”

Section: Introductionmentioning

confidence: 82%

“…[ 9 ] In fact, lead intoxication shares some features with hereditary P5N deficiency. [ 10 ] As reported by previous studies, elevated blood lead concentration is associated with hematologic findings such as BS of red cells and hemolytic anemia, and it has been proven that decreased P5N activity is partially responsible for these findings. [ 10 11 ] Also, high blood lead level (BLL) could damage the majority of soft tissues mainly the liver.…”

Section: Introductionmentioning

confidence: 82%

“…[ 10 11 ] Also, high blood lead level (BLL) could damage the majority of soft tissues mainly the liver. [ 10 ] The central role of hepatic tissue in detoxification emphasizes the importance of liver function in clearance of xenobiotics especially in drug-addicted individuals. Meanwhile, lead poisoning impairs detoxification of xenobiotics in liver which may result in more complicated problems in drug abusers.…”

Section: Introductionmentioning

confidence: 99%

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Lead poisoning in opium-addicted subjects, its correlation with pyrimidine 5′-nucleotidase activity and liver function tests

2019

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Background:Lead may be added to the opium by drug smugglers. It can cause elevated blood lead level (BLL) in opium-addicted patients. Erythrocyte pyrimidine 5′-nucleotidase (P5N) activity is susceptible to high BLL. The aim of this study was to find out whether opium-addicted patients show erythropathy and elevated liver enzymes explainable by high BLL and decreased P5N activity.Methods:Forty orally opium-addicted subjects and 40 normal healthy volunteers were enrolled in this study. BLL was measured in whole blood specimens using atomic absorption spectrometry instrumentation. Enzymatic activity, protein amount of P5N, and erythrocyte purine/pyrimidine ratio were determined. Blood films were analyzed for the presence of basophilic stippling of red cells and hemolytic anemia. The level of liver function enzymes was measured.Results:The mean BLL for opium-addicted patients was significantly higher than control group (P < 0.001). On the contrary, P5N activity showed a valid decrease in opium-addicted patients when compared with control group (P < 0.001). In line with repressed P5N activity, erythrocyte purine/pyrimidine ratio in patients was lower than control group (P < 0.001). A statistically significant reverse correlation was found between BLL and P5N activity (P < 0.05, r = −0.85). The prevalence of both basophilic stippling (P < 0.001, z = 6.62) and hemolytic anemia (P < 0.001, z = 6.52) in study population was significantly associated with elevated BLL. We could not find any significant correlation between serum level of liver enzymes and BLL.Conclusions:Opium-addicted patients in Tehran, Iran, are at high risk of lead poisoning which may result in hematologic problems and possibly hepatic damage.

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Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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Lead poisoning in opium-addicted subjects, its correlation with pyrimidine 5′-nucleotidase activity and liver function tests

2019

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“…The model of acute lead poisoning is established by giving experimental animals a single administration of a lead compound ( 27 ) whereas establishment of the model of chronic lead poisoning involves multiple administrations of a lead compound ( 28 ).Rats were divided in random to groups (five rats/group). The rat model of chronic lead poisoning was established by daily intragastric Pb(Ac) 2 injections at a high dosage of 20 mg/kg body weight for 1 week, and also at a low dosage (5 mg/kg body weight) of Pb(Ac) 2 solution for 30 days after a recovery period of 3 days.…”

Section: Methodsmentioning

confidence: 99%

A novel mushroom (Auricularia polytricha) glycoprotein protects against lead-induced hepatoxicity, promotes lead adsorption, inhibits organ accumulation of lead, upregulates detoxifying proteins, and enhances immunoregulation in rats

Zhao

Gao²,

Wang

et al. 2023

Front. Nutr.

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IntroductionLead is a ubiquitous environmental and industrial pollutant. Its nonbiodegradable toxicity induces a plethora of human diseases. A novel bioactive glycoprotein containing 1.15% carbohydrate, with the ability of adsorbing lead and effecting detoxification, has been purified from Auricularia polytricha and designated as APL. Besides, its mechanisms related to regulation of hepatic metabolic derangements at the proteome level were analyzed in this study.MethodsChromatographic techniques were utilized to purify APL in the current study. For investigating the protective effects of APL, Sprague-Dawley rats were given daily intraperitoneal injections of lead acetate for establishment of an animal model, and different dosages of APL were gastrically irrigated for study of protection from lead detoxification. Liver samples were prepared for proteomic analyses to explore the detoxification mechanisms.Results and discussionThe detoxifying glycoprotein APL displayed unique molecular properties with molecular weight of 252-kDa, was isolated from fruiting bodies of the edible fungus A. polytricha. The serum concentrations of lead and the liver function biomarkers aspartate and alanine aminotransferases were significantly (p<0.05) improved after APL treatment, as well as following treatment with the positive control EDTA (300 mg/kg body weight). Likewise, results on lead residue showed that the clearance ratios of the liver and kidneys were respectively 44.5% and 18.1% at the dosage of APL 160 mg/kg, which was even better than the corresponding data for EDTA. Proteomics disclosed that 351 proteins were differentially expressed following lead exposure and the expression levels of 41 proteins enriched in pathways mainly involved in cell detoxification and immune regulation were normalized after treatment with APL-H. The results signify that APL ameliorates lead-induced hepatic injury by positive regulation of immune processing, and suggest that APL can be applied as a therapeutic intervention of lead poisoning in clinical practice. This report represents the first demonstration of the protective action of a novel mushroom protein on lead-elicited hepatic toxicity.

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“…Compared with the most advanced X-ray fluorescence technology, the detection limit, detection speed, and detection accuracy of CO 2 laser-induced spectroscopy are comparable, and the hardware of CO 2 laser-induced spectroscopy has a lower price and is expected to replace X-ray fluorescence spectroscopy to realize the detection of lead in glass. Tian et al [52] used Fourier transform infrared spectroscopy based on principal component analysis (PCA) and partial least squares (PLS-DA) to study the biochemical changes in plasma during acute lead poisoning (ALP) in rats. The researchers first collected a large number of plasma samples from rats with and without ALP and found that the corresponding biochemical changes between plasma and lead can be used as potential spectral biomarkers for the diagnosis of lead poisoning.…”

Section: Spectrometrymentioning

confidence: 99%

Detection Techniques for Lead Ions in Water: A Review

Hu²,

Cheng

et al. 2023

Molecules

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Lead pollution has increasingly become the focus of environmental pollution, which is a great harm to the ecological environment and human health. Strict control of the emission of lead pollutants and accurate monitoring of lead are very important. The lead ion detection technologies are introduced here, including spectrophotometry, electrochemical method, atomic absorption spectrometry, and other detection methods, and the methods’ applicability, the advantages, and disadvantages are discussed. The detection limits of voltammetry and atomic absorption spectrometry are as low as 0.1 μg/L, and those of atomic absorption spectrometry are as low as 2 μg/L. The detection limit of photometry is higher (0.01 mg/L), but this method can be achieved in most laboratories. The application of different extraction pretreatment technologies in lead ion detection is introduced. The new technologies develop at home and abroad, such as precious metal nanogold technology, paper microfluidic technology, fluorescence molecular probe technology, spectroscopy, and other emerging technologies in recent years, are reviewed, and the principle and application of various technologies are expounded.

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A Plasma Biochemical Analysis of Acute Lead Poisoning in a Rat Model by Chemometrics-Based Fourier Transform Infrared Spectroscopy: An Exploratory Study

Cited by 13 publications

References 34 publications

Lead poisoning in opium-addicted subjects, its correlation with pyrimidine 5′-nucleotidase activity and liver function tests

Lead poisoning in opium-addicted subjects, its correlation with pyrimidine 5′-nucleotidase activity and liver function tests

A novel mushroom (Auricularia polytricha) glycoprotein protects against lead-induced hepatoxicity, promotes lead adsorption, inhibits organ accumulation of lead, upregulates detoxifying proteins, and enhances immunoregulation in rats

Detection Techniques for Lead Ions in Water: A Review

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