A plasma membrane antigen highly associated with oat‐cell carcinoma of the lung and undetectable in normal adult tissue

Ce, Bell; Seetharam, Shakuntla

doi:10.1002/ijc.2910180509

Cited by 18 publications

(5 citation statements)

References 17 publications

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“…Several groups have prepared polyclonal antibodies with potential specificity for lung cancer by using standard immunologic methods (1)(2)(3)(4)(5)(6)(7)(8). The development of somatic cell hybrid technology for the production of monoclonal antibodies (9) offers a different approach, and several monoclonal antibodies have been reported with various degrees of specificity to a variety of human neoplasms (10)(11)(12)(13)(14)(15)(16)(17).…”

mentioning

confidence: 99%

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Cuttitta¹,

Rosen²,

Gazdar³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

121

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Monoclonal antibodies with selectivity for human lung cancer were produced by immunizing BALB/c mice with an established line of human small cell lung cancer (NCI-H69) and fusing the mouse spleen cells to mouse myeloma line X63-Ag8.653. The resulting hybrid cells were initially screened' by immunoautoradiography for production of antibodies that would react with NCI-H69 and another small cell lung cancer line (NCI-H128) but not its autologous B-lymphoblastoid line (NCI-H128BL). Stable monoclonal antibody-producing lines were isolated by repeated cloning. Three independently derived monoclonal antibodies, designated 525A5, 534F8, and 538F12, were found to react with three of the major types of human lung cancer (small cell, adenocarcinoma, and squamous carcinoma). They did not react with bronchioloalveolar and large cell lung cancers, myeloma, lymphomas, leukemias, osteogeneic sarcoma, mesothelioma, hypernephroma, malignant melanoma, simian virus 40-transformed human fetal lung cells, skin fibroblast lines, human B-lymphoblastoid lines, human erythrocytes, and rodent cells. Interestingly, these antibodies also bound to three out ofthree human neuroblastomas and two out of three breast cancers but failed to react with mouse neuroblastoma and rat pheochromocytoma. The monoclonal antibodies reacted with human small cell lung cancer tumors obtained at autopsy, but had insignificant reactions with normal human lung, liver, spleen, and skeletal muscle. We conclude that monoclonal antibodies have been generated that react with common antigenic determinants expressed on several human lung cancer types, neuroblastoma, and some breast cancers, but are not detectable by our current assays on a variety of other human tumors or normal adult human tissues. Such antibodies are of potential clinical and biological importance.Antibodies with sufficient specificity for human lung cancer are potentially important diagnostic and therapeutic tools. Several groups have prepared polyclonal antibodies with potential specificity for lung cancer by using standard immunologic methods (1-8). The development of somatic cell hybrid technology for the production of monoclonal antibodies (9) offers a different approach, and several monoclonal antibodies have been reported with various degrees of specificity to a variety of human neoplasms (10-17). In this report, we describe the use of this technology to prepare antibodies with specificity for human lung cancer. As a first approach, we wanted to make monoclonal antibodies that react selectively with lung tumors but not with autologous histocompatibility antigens. Thus, we immunized mice with a line of small cell lung cancer (SCLC) and screened the resultant hybrid cell antibodies for reactivity against the immunizing line and another SCLC line and for lack ofreactivity with an autologous B-lymphoblastoid cell line. Using this strategy, we have isolated, cloned, and developed stable hybrid cell lines producing antibodies that detect determinants expressed on human SCLC, and to a lesser ...

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mentioning

confidence: 99%

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Cuttitta¹,

Rosen²,

Gazdar³

et al. 1981

Proc. Natl. Acad. Sci. U.S.A.

121

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Section: Methodsmentioning

confidence: 99%

“…These were raised to extracts as described in Bell & Seetharam (1976), Gaffar et al (1979, Gennings et al (1979, Gropp et al (1979, Ibrahim et al (1980), Lamerz et al (1979), McIntire & Sizaret (1974), Mohr et al (1974), Veltri et al (1977), Wolf (1978. In the case of Ford et al (1980), the antisera were raised to viable bronchial tumour cells in culture. It may be noted that there are wide differences in the normal tissues with which Electroimmunoprecipitation.-Two methods of electrophoretic separation were used: (1) Fused-rocket immunoelectrophoresis (IEP), (2) crossed IEP with intermediate gel.…”

Section: Methodsmentioning

confidence: 99%

Collaborative study of bronchial tumour-associated antigens

Gennings¹,

Bagshawe²,

Axelsen³

et al. 1981

Br J Cancer

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Summary.-Eleven groups of workers submitted a total of 21 bronchial tumourassociated antigen preparations and 19 antisera for comparative studies. Many of the antisera proved to be polyspecific despite absorption procedures. Most of the antigen preparations contained some material reactive towards a reference antiserum to normal human serum proteins.While it appeared that no participants were studying identical antigen-antibody reactions, several cross-reactivities were identified in the antisera. When immune reactions to CEA, AFP, NCA, ferritin, lactoferrin, human pepsin and gastricsin, and the pregnancy proteins, SP1 and SP3 were excluded by use of reference antisera and electroimmunoprecipitation methods, there remained 5 antigen-antibody reactions defining unique antigens. The clinical usefulness of any of these 5 antigens has yet to be determined.

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“…Bell used IHC to demonstrate an antigen highly associated with oat cell carcinoma and undetectable in normal adult tissue (148). The antigen was also characteristic of certain normal neural crest-derived cells in the peripheral nervous system (149).…”

Section: Markers Of Lung Tumorsmentioning

confidence: 99%

Immunohistochemical techniques and their applications in the histopathology of the respiratory system.

Linnoila¹,

Petrusz²

1984

Environ Health Perspect

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Subsequent to the first report in the 1940s on incubation of tissue sections with fluorescein-conjugated antibodies for localization of antigens, a great number of modifications were introduced to improve the validity of immunohistochemistry which has become a growingly popular tool. The use of immunoenzymatic techniques eliminates the need for expensive fluorescence microscopy equipment, the lack of permanency of preparations and the lack of electron density required in ultrastructural localization of antigens. Regardless of the technique, it is also important to choose a correct fixation which allows the proper preservation of antigens and morphology and the penetration of antibodies through the entire thickness of the preparation. A variety of immunohistochemical techniques have been applied to study several components of the lung, such as collagen, surface active material, lung specific antigens, and enzymes and the detection of tumor markers, immunoglobulins and infectious agents in the respiratory system which is reviewed. The large surface area and the multiplicity of cell types provided by the respiratory tract epithelium of humans for exposure to microbial as well as toxic substances in the environment make this organ system very vulnerable but a good early indicator of adverse health effects. Immunohistochemistry provides valuable information complementary to the immunochemical and biochemical characterization of this barrier.

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A plasma membrane antigen highly associated with oat‐cell carcinoma of the lung and undetectable in normal adult tissue

Cited by 18 publications

References 17 publications

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.

Collaborative study of bronchial tumour-associated antigens

Immunohistochemical techniques and their applications in the histopathology of the respiratory system.

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