A plasmid DNA vaccine encoding the extracellular domain of porcine endoglin induces anti-tumour immune response against self-endoglin-related angiogenesis in two liver cancer models

Jiao, Jie-ge; Li, Y.-N.; Wang, H.; Liu, Q.; Cao, Jasmin; Bai, Ruizhen; Huang, Feng‐Ying

doi:10.1016/j.dld.2006.04.014

Cited by 17 publications

(15 citation statements)

References 29 publications

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“…After 14 d, mice were injected intravenously with 100 μL of 100 mg/kg FITC-dextran solution. Beads were surgically removed and FITC-dextran was quantified against a standard curve of FITC-dextran as previously described [9][10][11][12] .…”

Section: Alginate Encapsulation Assaymentioning

confidence: 99%

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Immunotherapy of hepatoma with a monoclonal antibody against murine endoglin

Tan¹

2007

WJG

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AIM:To explore the capability of a monoclonal antibody (mAb) against murine endoglin to inhibit tumor angiogenesis and suppression of hepatoma growth in murine models. METHODS:A monoclonal antibody against murine endoglin was purified by affinity chromatography and passively transfused through tail veins in two murine hepatoma models. Tumor volume and survival time were observed at three-day intervals for 48 d. Microvessels in tumor tissues were detected by immunohistochemistry against CD31, and angiogenesis in vivo was determined by alginate encapsulated assay. In addition, tumor cell apoptosis was detected by TUNEL assay. RESULTS:Passive immunotherapy with anti-endoglin mAb could effectively suppress tumor growth, and prolonged the survival time of hepatoma-bearing mice. Angiogenesis was apparently inhibited within the tumor tissues, and the vascularization of alginate beads was also reduced in the mice passively transfused with antiendoglin mAb. In addition, increased apoptotic cells were observed within the tumor tissues from the mice passively transfused with anti-endoglin mAb. CONCLUSION:Passive immunotherapy with antiendoglin mAb effectively inhibits tumor growth via inhibiting tumor angiogenesis and increasing tumor cell apoptosis, which may be highly correlated with the blockage of endoglin-related signal pathway induced by anti-endoglin mAb.

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Section: Alginate Encapsulation Assaymentioning

confidence: 99%

“…To determine microvessel density (MVD), frozen sections of tumor tissues were prepared, and subjected to immunohistochemical staining for CD31 as previously described [9][10][11][12] . Briefly, frozen sections were fixed in acetone, incubated, and stained with an antibody reactive to CD31.…”

Section: Immunohistochemical Detection Of Tumor Microvessel Densitymentioning

confidence: 99%

Immunotherapy of hepatoma with a monoclonal antibody against murine endoglin

Tan¹

2007

WJG

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“…Therapeutic vaccination of mice with an extracellular portion of porcine endoglin was shown to induce immune responses directed against colorectal and lung cancers. The generation of such responses depended on functional activity of CD4+ T-lymphocytes and resulted in the appearance of endoglin-binding autoantibodies [32,33]. A significant enhancement of antitumor effect was achieved when protein vaccination was combined with DNA vaccination.…”

Section: Xenovaccinotherapy In Animal Modelsmentioning

confidence: 97%

“…An antitumor effect of DNA vaccination with porcine endoglin was also demonstrated in a murine model of liver cancer. This effect was mediated by both cellular and humoral immune reactions [35]. Tie-2 is an endothelium-specific receptor tyrosine kinase known to play a key role in tumor angiogenesis.…”

Section: Xenovaccinotherapy In Animal Modelsmentioning

confidence: 99%

Xenovaccinotherapy for Cancer

Селедцов¹,

Shishkov²,

Селедцова³

2011

Current Cancer Treatment - Novel Beyond Conventional Approaches

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“…17,18) Some studies have shown that immunization with plasmid DNA encoding angiogenesis-related antigens can inhibit tumor associated angiogenesis. [19][20][21][22] These DNA vaccines can induce either antigen-specific humoral or cellular immune responses. The vaccination procedure generated antibodies or T-cell-mediated cytotoxicity against the targeted cells can inhibit tumor growth and lead to tumor rejection.…”

mentioning

confidence: 99%