A plasmonic nanoledge array sensor for detection of anti-insulin antibodies of type 1 diabetes biomarker

Abstract

Here we present a plasmonic nanoledge device with high sensitivity and selectivity used to detect protein biomarkers simply by functionalizing the device, which specifically binds to particular biomolecule or biomarkers. We employ this plasmonic nanoledge device for the detection of anti-insulin antibodies of type 1 diabetes (T1D) in buffer and human serum at the range of pg ml −1 to 100 ng ml −1 . The signal transduction is based on the extraordinary optical transmission (EOT) through the nanoledge array and the optical spectral changes with the biological binding reaction between the surface functionalized insulin with anti-insulin antibody. Control experiments indicate little interferences from the human serum background and addition of other proteins such as bovine serum albumin (BSA) and epidermal growth factor (EGF) at 20 ng ml −1 . The high sensitivity, specificity and easy adaptability of the plasmonic device offer new opportunities in biosensing and diagnostic applications for T1D.

Molecularly Imprinted Plasmonic Sensors as Nano-Transducers: An Effective Approach for Environmental Monitoring Applications

“…Over the years, our scientific research group has garnered knowledge in developing plasmonic nanostructured sensors for detecting both small molecules and biomolecules in various matrices. The focal point of our work is to explore nanomaterials and nanostructures to develop efficient analytical tools for rapid testing and monitoring processes and to help address challenges that traditional standard methods encounter in detecting a variety of molecular compounds, such as OPPs [18], protein biomarkers [19][20][21][22] and DNAs [23,24].…”

A Design Strategy for Surface Nanostructures to Realize Sensitive Refractive-Index Optical Sensors

“…The requirement of an elaborate optical configuration that includes a total-reflection setup is a drawback of SPR sensors. Therefore, the development of refractive-index sensors that can operate in compact and simple optical configurations using surface nanostructures has been explored in the visible and near-infrared ranges [3][4][5][6][7][8][9][10][11][12][13]. From a similar motive, fiber-based refractive-index sensors were studied [14][15][16][17][18]; these sensors have long light propagating paths and high responsivity in the near-infrared range, while the sensing range is mostly limited to a narrow refractive-index range of 1.…”

“…If periodic structures are chosen, the next step is whether or not to conduct designs based on dispersion: (i) if yes, feasible periodic structures realizing specific dispersions are designed, tested using a numerical simulation method, and evaluated in terms of performance as a refractive-index sensor; (ii) if no, nanostructures are designed based on interest and/or preference by each researcher. From this reason, Mie resonances in metallic nanoparticles [4,5], local resonances in metallic/dielectric nanostructures [3,[6][7][8][9][10], and bound states in the continuum (BIC) [11][12][13] have often been studied. Artificial surfaces were designed by assembling the nanostructures on a substrate; subsequently, the surface structures were tested using the simulation method, and their performance was evaluated.…”