A pleiotropic chemoreceptor facilitates the production and perception of mating pheromones

Vernier, Cassondra; Leitner, Nicole; Zelle, Kathleen M.; Foltz, Merrin; Dutton, Sophia; Liang, Xitong; Halloran, Sean; Millar, Jocelyn G.; Ben‐Shahar, Yehuda

doi:10.1016/j.isci.2022.105882

Cited by 5 publications

(2 citation statements)

References 89 publications

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“…Previously, desat1 has been shown to be involved in both pheromone production and perception ( 71 ). A recent study found that the Gr8a gene, which belongs to the gustatory receptor gene family, is expressed specifically in male oenocytes and plays a role in the synthesis of certain male CHCs by regulating the level of Desat1 ( 72 ). On the basis of our RNA-seq analysis, desat1 is a likely downstream target of fru in the oenocyte.…”

Section: Discussionmentioning

confidence: 99%

Integrating lipid metabolism, pheromone production and perception by Fruitless and Hepatocyte Nuclear Factor 4

et al. 2023

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Sexual attraction and perception are crucial for mating and reproductive success. In Drosophila melanogaster , the male-specific isoform of Fruitless (Fru), Fru M , is a known master neuro-regulator of innate courtship behavior to control the perception of sex pheromones in sensory neurons. Here, we show that the non–sex-specific Fru isoform (Fru COM ) is necessary for pheromone biosynthesis in hepatocyte-like oenocytes for sexual attraction. Loss of Fru COM in oenocytes resulted in adults with reduced levels of cuticular hydrocarbons (CHCs), including sex pheromones, and show altered sexual attraction and reduced cuticular hydrophobicity. We further identify Hepatocyte nuclear factor 4 ( Hnf4 ) as a key target of Fru COM in directing fatty acid conversion to hydrocarbons. Fru or Hnf4 depletion in oenocytes disrupts lipid homeostasis, resulting in a sex-dimorphic CHC profile that differs from doublesex- and transformer- dependent CHC dimorphism. Thus, Fru couples pheromone perception and production in separate organs to regulate chemosensory communications and ensure efficient mating behavior.

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Section: Discussionmentioning

confidence: 99%

Integrating lipid metabolism, pheromone production and perception by Fruitless and Hepatocyte Nuclear Factor 4

et al. 2023

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“…In Helicoverpa armigera , female moths release cis-11-Hexadecenol (Z11-16:OH) to repel males and avoid non-optimal mating 13 . Likewise, in Drosophila , Gr8-associated alkenes inhibit courtship behaviors 14 . Collectively, these findings suggest that the inverse agonist may be widespread in insects.…”

Section: Discussionmentioning

confidence: 99%

The dual coding of a single sex pheromone receptor in regulating the mating behavior of Asian HoneybeeApis cerana

Ke,

Bohbot,

Chi

et al. 2023

Preprint

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In honeybee society, a virgin queen usually mates only once with several drones before founding a colony. For the rest of her prolific life, she will not engage in subsequent mating events. In the Asian honeybeeApis cerana, the mechanisms controlling this reproductive strategy involves the queen-released mandibular pheromone (QMP). This pheromone blend regulates the physiology and reproductive behavior of workers and drones. Its main component, 9-oxo-(E)-2-decenoic acid (9-ODA), acts as a sex pheromone and attracts drones. However, how the QMP prevents additional mating later in the queen’s life remains elusive. Using behavioral and chemical analysis, we show that the QMP component methylp-hydroxybenzoate (HOB) released by mated queens inhibits male attraction to 9-ODA. Furthermore,in vivoelectroantennogram and single sensillum recording data indicated that HOB alone significantly reduces the spontaneous spike activity of 9-ODA-sensitive olfactory receptor neurons (ORNs). To explore the molecular mechanism underlying this inverse effect, we conducted qPCR and in situ hybridization assays. The results indicated thatAcerOr11is specifically expressed in sensilla placodea from the drone’s antennae, which are the sensilla that narrowly respond to both 9-ODA and HOB. We then cloned and expressedAcerOr11in a Xenopus oocyte expression system, whereAcerOr11induced robust inward (regular) currents in response to 9-ODA. Intriguingly, HOB induced inverse currents in a dose-dependent manner. This suggests that HOB may act as an inverse agonist againstAcerOr11, providing additional odor-coding information. Based on these findings, we propose a model in whichAcerOr11function as a dual modulators in regulating the mating behavior ofA. cerana. The inverse agonist, HOB, can help manage the population dynamics of honeybees in apiculture.

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Beyond 'push–pull': unraveling the ecological pleiotropy of plant volatile organic compounds for sustainable crop pest management

Munawar,

Zhu,

Machado

et al. 2023

Crop Health

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Plants produce an array of different volatile organic compounds (VOCs) that have diverse eco-physiological functions and agricultural applications. Hence, the potential of VOCs as a natural and eco-friendly solution to increase crop productivity has garnered substantial attention. In particular, VOC-based pest management measures, such as Push–Pull, have been widely investigated in different cropping systems. However, our understanding of how the release and use of these VOCs impact agro-ecosystems beyond the targeted effects is still very limited. Therefore, studying the functions of plant VOCs from the perspective of pleiotropy may offer insights into optimizing and improving the effectiveness of VOC-based agronomic practices. Here, we discuss the current literature on the pleiotropic functions of plant VOCs, illustrate the underlying mechanism for their production and propose a systematic perspective for the usage of plant VOCs to enhance the sustainable management of crop health.

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A pleiotropic chemoreceptor facilitates the production and perception of mating pheromones

Cited by 5 publications

References 89 publications

Integrating lipid metabolism, pheromone production and perception by Fruitless and Hepatocyte Nuclear Factor 4

Integrating lipid metabolism, pheromone production and perception by Fruitless and Hepatocyte Nuclear Factor 4

The dual coding of a single sex pheromone receptor in regulating the mating behavior of Asian HoneybeeApis cerana

Beyond 'push–pull': unraveling the ecological pleiotropy of plant volatile organic compounds for sustainable crop pest management

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