A Plug and Play Approach for the Decoration of Nanoparticles With Recombinant Proteins

Ferrari, Enrico

doi:10.2217/nnm-2018-0261

Cited by 2 publications

(2 citation statements)

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“…Gold nanoparticles coated with α-amylase were prepared following passive adsorption, producing stable and active enzyme-nanoparticle conjugates without need for chemical modification of the enzyme (Figure ). , The citrate-capped AuNP are used to form αA-AuNP and the excess protein is removed by centrifugation. The final concentration of nanoparticles is 3.9 × 10 12 NP/mL, with an estimated 35 μg/mL load of α-amylase (see Supporting Information, SI1).…”

Section: Results and Discussionmentioning

confidence: 99%

“…Single fibers and woven textile were sampled from cotton textile and mounted on aluminum stubs. ESEM-EDX data were recorded before and after the application of dispersions, allowing 30 fibers, while backscattered electron (BSE) images were used to study the surface distribution and the penetration depth of gold nanoparticles modified with amylase in cross sections of cotton specimens. 2.6.…”

Section: Methodsmentioning

confidence: 99%

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Immobilized Enzymes on Gold Nanoparticles: From Enhanced Stability to Cleaning of Heritage Textiles

Gherardi

Turyanska

Ferrari

et al. 2019

ACS Appl. Bio Mater.

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Enzyme-based treatments are used in heritage conservation for the effective removal of glues and other damaging organic layers from the surfaces of historic and artisitc works. Despite their potential, however, the application of enzymatic treatments is currently limited due to their poor efficiency, and low operational and environmental stability. We demonstrate the use of α-amylase immobilized on gold nanoparticles to improve the efficacy of enzymatic treatments enchancing both the reactivity and the stability of the formulations. Gold nanoparticles coated with α-amylase exhibit significant advantages compared to free enzymes.We report up to 5-times greater resistance to environmental changes, up to 2-times higher efficacy towards removal of starch-based glues from textile, and deeper penetration through the fibres, without causing damage or inducing salt precipitation. These results offer exciting prospects for the development of novel enzymatic formulations, both for heritage conservation and the wider application of enzymes, such as in medicine, the detergent industry and green chemistry.

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Section: Results and Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%