A Pocket‐Sized Convective PCR Thermocycler

Agrawal, Nitin; Hassan, Yassin A.; Ugaz, Victor M.

doi:10.1002/anie.200700306

Cited by 75 publications

(35 citation statements)

References 16 publications

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“…Several 'portable' PCR instruments (e.g. Agrawal et al 2007) have been developed that may enable analysis to be undertaken at the sampling site, although the DNA extraction step and prevention of contamination while working in the field remain a challenge.…”

Section: Microbial Source Trackingmentioning

confidence: 99%

Molecular genetic tools for environmental monitoring of New Zealand's aquatic habitats, past, present and the future

Wood

Smith

Banks

et al. 2013

New Zealand Journal of Marine and Freshwater Research

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Section: Microbial Source Trackingmentioning

confidence: 99%

Molecular genetic tools for environmental monitoring of New Zealand's aquatic habitats, past, present and the future

Wood

Smith

Banks

et al. 2013

New Zealand Journal of Marine and Freshwater Research

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“…There are already nano-technology-based, battery-operated, mini-PCR machines available 101 which could provide a DNA profile of a plant material within an hour. This information could be compared on-site, or through satellite communications with large databases currently being established.…”

Section: A Mini-pcr Machinementioning

confidence: 99%

Sustainable drugs and global health care

Cordell¹

2010

Planta Med

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Each day, Earth's finite resources are being depleted for energy, for material goods, for transportation, for housing, and for drugs. As we evolve scientifically and technologically, and as the population of the world rapidly approaches 7 billion and beyond, among the many issues with which we are faced is the continued availability of drugs for future global health care. Medicinal agents are primarily derived from two sources, synthetic and natural, or in some cases, as semi-synthetic compounds, a mixture of the two. For the developed world, efforts have been initiated to make drug production "greener", with milder reagents, shorter reaction times, and more efficient processing, thereby using less energy, and reactions which are more atom efficient, and generate fewer by-products. However, most of the world's population uses plants, in either crude or extract form, for their primary health care. There is relatively little discussion as yet, about the long term effects of the current, non-sustainable harvesting methods for medicinal plants from the wild, which are depleting these critical resources without concurrent initiatives to commercialize their cultivation. To meet future public health care needs, a paradigm shift is required in order to adopt new approaches using contemporary technology which will result in drugs being regarded as a sustainable commodity, irrespective of their source. In this presentation, several approaches to enhancing and sustaining the availability of drugs, both synthetic and natural, will be discussed, including the use of vegetables as chemical reagents, and the deployment of integrated strategies involving information systems, biotechnology, nanotechnology, and detection techniques for the development of medicinal plants with enhanced levels of bioactive agents.

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“…The first research group used one plastic hollow cylinder as a reagent container and clamped the two opening ends of the cylinder by metal blocks [9,10]. The temperature gradient was created when heating metal blocks with different temperatures.…”

Section: Introductionmentioning

confidence: 99%

Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection

et al. 2017

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Polymerase chain reaction (PCR) has been one of the principal techniques of molecular biology and diagnosis for decades. Conventional PCR platforms, which work by rapidly heating and cooling the whole vessel, need complicated hardware designs, and cause energy waste and high cost. On the other hand, partial heating on the various locations of vessels to induce convective solution flows by buoyancy have been used for DNA amplification in recent years. In this research, we develop a new convective PCR platform, capillary loop convective polymerase chain reaction (clcPCR), which can generate one direction flow and make the PCR reaction more stable. The U-shaped loop capillaries with 1.6 mm inner diameter are designed as PCR reagent containers. The clcPCR platform utilizes one isothermal heater for heating the bottom of the loop capillary and a CCD device for detecting real-time amplifying fluorescence signals. The stable flow was generated in the U-shaped container and the amplification process could be finished in 25 min. Our experiments with different initial concentrations of DNA templates demonstrate that clcPCR can be applied for precise quantification. Multiple sample testing and real-time quantification will be achieved in future studies.

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A Pocket‐Sized Convective PCR Thermocycler

Cited by 75 publications

References 16 publications

Molecular genetic tools for environmental monitoring of New Zealand's aquatic habitats, past, present and the future

Molecular genetic tools for environmental monitoring of New Zealand's aquatic habitats, past, present and the future

Sustainable drugs and global health care

Development of Capillary Loop Convective Polymerase Chain Reaction Platform with Real-Time Fluorescence Detection

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