A point mutation in the photosystem I P700 chlorophyll a apoprotein A1 gene confers variegation in Helianthus annuus L

Azarin, Kirill; Усатов, А. В.; Makarenko, Maksim; Козел, Н. В.; Kovalevich, A. A.; Dremuk, I. A.; Yemelyanova, Anna; Logacheva, Mariya D.; Федоренко, А. Г.; Аверина, Н. Г.

doi:10.1007/s11103-020-00997-x

Cited by 24 publications

(19 citation statements)

References 89 publications

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“…Previous studies have reported that the fine structure of chloroplasts altered in the yellow sectors of variegated leaves. For example, chloroplasts in the green sectors of H. annuus L showed well-organized stromal and granal thylakoids, while plastids in the yellow sectors only contained poorly developed granum-stroma thylakoid membrane system [12]. In our study, TEM revealed a strong contrast in chloroplast ultrastructure between juvenile yellow leaves, yellow sectors and green sectors of the variegated leaves.…”

Section: Discussioncontrasting

confidence: 40%

“…Whereas, a recent study noticed that mutations in SCO2 could affect development of true leaves in Arabidopsis under short-day conditions, and induce leaf variegation in Lotus japonicas [11]. In plant species other than Arabidopsis, it was found that mutation in chlorophyll a apoprotein A1 gene could lead to variegation in Helianthus annuus L. [12]. In Cymbidium sinense, the ethylene response factors (ERFs) gene CsERF2 played crucial roles in leaf variegation [13].…”

Section: Introductionmentioning

confidence: 99%

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Cytological and Transcriptomic Analysis Provide Insights into the Formation of Variegated Leaves in Ilex × altaclerensis ‘Belgica Aurea’

Zhang

Huang

Zhou

et al. 2021

Plants

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Ilex × altaclerensis ‘Belgica Aurea’ is an attractive ornamental plant bearing yellow-green variegated leaves. However, the mechanisms underlying the formation of leaf variegation in this species are still unclear. Here, the juvenile yellow leaves and mature variegated leaves of I. altaclerensis ‘Belgica Aurea’ were compared in terms of leaf structure, pigment content and transcriptomics. The results showed that no obvious differences in histology were noticed between yellow and variegated leaves, however, ruptured thylakoid membranes and altered ultrastructure of chloroplasts were found in yellow leaves (yellow) and yellow sectors of the variegated leaves (variegation). Moreover, the yellow leaves and the yellow sectors of variegated leaves had significantly lower chlorophyll compared to green sectors of the variegated leaves (green). In addition, transcriptomic sequencing identified 1675 differentially expressed genes (DEGs) among the three pairwise comparisons (yellow vs. green, variegation vs. green, yellow vs. variegation). Expression of magnesium-protoporphyrin IX monomethyl ester (MgPME) [oxidative] cyclase, monogalactosyldiacylglycerol (MGDG) synthase and digalactosyldiacylglycerol (DGDG) synthase were decreased in the yellow leaves. Altogether, chlorophyll deficiency might be the main factors driving the formation of leaf variegation in I.altaclerensis ‘Belgica Aurea’.

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Section: Discussioncontrasting

confidence: 40%

Section: Introductionmentioning

confidence: 99%

Cytological and Transcriptomic Analysis Provide Insights into the Formation of Variegated Leaves in Ilex × altaclerensis ‘Belgica Aurea’

Zhang

Huang

Zhou

et al. 2021

Plants

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“…KEGG enrichment analysis with the 20 most enriched KEGG terms shown (b). High and low p-values are represented by blue and red, respectively variegated mutants is either absent or very low [25]. These results suggest that inhibition of POR in the yellow leaf mutant reduces Chl content and leads to the yellow leaf phenotype.…”

Section: Discussionmentioning

confidence: 81%

“…It has been reported that mutations in the PORB gene lead to a yellow/white leaf variegation phenotype in rice [ 5 ]. In addition, in Arabidopsis , the transcriptional activity of PORB and PORC in the yellow tissues of variegated mutants is either absent or very low [ 25 ]. These results suggest that inhibition of POR in the yellow leaf mutant reduces Chl content and leads to the yellow leaf phenotype.…”

Section: Discussionmentioning

confidence: 99%

Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm

et al. 2021

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Background Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant “WY16–13” was identified, which exhibited yellow-green leaf color during its entire growth period. However, current understanding of the molecular mechanism underlying Chl metabolism and chloroplast development of “WY16–13” is limited. Results Total Chl and carotenoid content in WY16–13 was reduced by 60.92 and 58.82%, respectively, as compared with its wild type parental line W16–13. Electron microscopic investigation revealed fewer chloroplasts per cell and looser stroma lamellae in WY16–13 than in W16–13. A comparative transcriptome profiling was performed using leaves from the yellow-green leaf type (WY16–13) and normal green-leaf type (W16–13). A total of 54.12 million (M) (WY16–13) and 56.17 M (W16–13) reads were generated. A total of 40,578 genes were identified from the mapped libraries. We identified 3882 differentially expressed genes (DEGs) in WY16–13 compared with W16–13 (i.e., 1603 upregulated genes and 2279 downregulated genes). According to the Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, these DEGs are involved in porphyrin and Chl metabolism [i.e., chlorophyllase (CLH), heme oxygenase (HO), chlorophyll (ide) b reductase (NYC), and protochlorophyllide oxidoreductase (POR) genes], carbohydrate metabolism, photosynthesis, and carbon fixation in photosynthetic organisms. Moreover, deficiency in Chl biosynthetic intermediates in WY16–13 revealed that the formation of the yellow-green phenotype was related to the disorder of heme metabolism. Conclusions Our results provide valuable insights into Chl deficiency in the yellow-green leaf mutant and a bioinformatics resource for further functional identification of key allelic genes responsible for differences in Chl content.

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“…It has been reported that mutations in the PORB gene lead to a yellow/white leaf variegation phenotype in rice [5]. In addition, in Arabidopsis, the transcriptional activity of PORB and PORC in the yellow tissues of variegated mutants is either absent or very low [26]. These results suggest that inhibition of POR in the yellow leaf mutant reduces Chl content and leads to the yellow leaf phenotype.…”

Section: Discussionmentioning

confidence: 91%

Characterization and Transcriptomic Analysis of a Novel Yellow-Green Leaf Wucai (Brassica Campestris L.) Germplasm

Nie

Yu-shan

Zhang

et al. 2020

Preprint

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Background: Leaf color mutants are the ideal materials to explore the pathways of chlorophyll (Chl) metabolism, chloroplast development, and photosynthesis system. In this study, a spontaneous yellow-green leaf wucai (Brassica campestris L.) mutant “WY16-13” was identified, which exhibited yellow-green leaf color during its entire growth period. However, current understanding of the molecular mechanism underlying Chl metabolism and chloroplast development of “WY16-13” is limited.Results: Comparative transcriptome profiling was performed using leaves from the yellow-green leaf type (WY16-13) and normal green-leaf type (W16-13). A total of 54.12 million (M) (WY16-13) and 56.17 M (W16-13) reads were generated. A total of 40,578 genes were identified from the mapped libraries. We identified 3,882 differentially expressed genes (DEGs) in WY16-13 compared with wild-type W16-13 (i.e., 1,603 upregulated genes and 2,279 downregulated genes). According to the Gene Ontology (GO) term and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses, these DEGs are involved in porphyrin and Chl metabolism [i.e., chlorophyllase (CLH), heme oxygenase (HO), chlorophyll(ide) b reductase (NYC), and protochlorophyllide oxidoreductase (POR) genes], carbohydrate metabolism, photosynthesis, and carbon fixation in photosynthetic organisms. Moreover, deficiency in Chl biosynthetic intermediates in the mutant revealed that the formation of the yellowing phenotype was related to the disorder of heme metabolism.Conclusions: Our results provide valuable insights into Chl deficiency in the yellow-green leaf mutant and a bioinformatics resource for further functional identification of key allelic genes responsible for differences in Chl content.

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A point mutation in the photosystem I P700 chlorophyll a apoprotein A1 gene confers variegation in Helianthus annuus L

Cited by 24 publications

References 89 publications

Cytological and Transcriptomic Analysis Provide Insights into the Formation of Variegated Leaves in Ilex × altaclerensis ‘Belgica Aurea’

Cytological and Transcriptomic Analysis Provide Insights into the Formation of Variegated Leaves in Ilex × altaclerensis ‘Belgica Aurea’

Characterization and transcriptomic analysis of a novel yellow-green leaf wucai (Brassica campestris L.) germplasm

Characterization and Transcriptomic Analysis of a Novel Yellow-Green Leaf Wucai (Brassica Campestris L.) Germplasm

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