A point-of-care single nucleotide variation assay based on strand-displacement-triggered recombinase polymerase amplification

Zhang, Lutan; Xu, Lulu; Zhang, Jian; Wang, Wenbo; Huang, Yanru; Zhou, Yixi; Yao, Xingmei; Liu, Zhaohui; Ge, Yunsheng

doi:10.1016/j.snb.2023.135075

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Modification Of Au Microelectrodes With Mesoporous Silica Th...1

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2024

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Cited by 3 publications

(1 citation statement)

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“…To facilitate the morphological characterization, mesoporous silica thin film was also modified onto an ITO electrode at a constant cathodic current density of 0.7 mA cm −2 for 10 s. 35 3 Results and discussion…”

Section: Modification Of Au Microelectrodes With Mesoporous Silica Th...mentioning

confidence: 99%

Ultrasensitive detection of dopamine using Au microelectrodes integrated with mesoporous silica thin films

Huang,

Zhou,

Zou

et al. 2024

Analyst

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Section: Modification Of Au Microelectrodes With Mesoporous Silica Th...mentioning

confidence: 99%

Ultrasensitive detection of dopamine using Au microelectrodes integrated with mesoporous silica thin films

Huang,

Zhou,

Zou

et al. 2024

Analyst

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Two‐Stage Mixed‐Dye‐Based Isothermal Amplification with Ribonuclease‐Cleavable Enhanced Probes for Dual‐Visualization Detection of SARS‐CoV‐2 Variants of Interest

Ding,

Wang,

Gui

et al. 2024

Advanced Science

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Rapid and visual detection of SARS‐CoV‐2 variants is vital for timely assessment of variant transmission in resource‐limited settings. Here, a closed‐tube, two‐stage, mixed‐dye‐based isothermal amplification method with ribonuclease‐cleavable enhanced probes (REP), termed REP‐TMAP, for dual‐visualization detection of SARS‐CoV‐2 variants including JN.1, BA.2, BA.4/5, and Delta is introduced. The first stage of REP‐TMAP is reverse transcription recombinase polymerase amplification and the second stage is dual‐visualization detection synergistically mediated by the REP and the mixed dyes of cresol red and hydroxy naphthol blue. In REP‐TMAP reaction, the color change under ambient light indicates SARS‐CoV‐2 infection, while the fluorescence change under blue light excitation specifies variant type. On detecting transcribed RNA of SARS‐CoV‐2 spike gene, this assay is rapid (within 40 min), highly sensitive (10–200 copies per reaction), and highly specific (identification of single‐base mutations). Furthermore, the assay has been clinically validated to accurately detect JN.1, BA.2, and BA.4/5 variants from 102 human oropharyngeal swabs. The proposed assay therefore holds great potentials to provide a rapid, dual‐visualization, sensitive, specific, point‐of‐care detection of SARS‐CoV‐2 variants and beyond.

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Point-of-care isothermal nucleic acid amplification tests: progress and bottlenecks for extraction-free sample collection and preparation

Wilkinson,

Barra,

Novak

et al. 2024

Expert Review of Molecular Diagnostics

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A point-of-care single nucleotide variation assay based on strand-displacement-triggered recombinase polymerase amplification

Cited by 3 publications

References 21 publications

Ultrasensitive detection of dopamine using Au microelectrodes integrated with mesoporous silica thin films

Ultrasensitive detection of dopamine using Au microelectrodes integrated with mesoporous silica thin films

Two‐Stage Mixed‐Dye‐Based Isothermal Amplification with Ribonuclease‐Cleavable Enhanced Probes for Dual‐Visualization Detection of SARS‐CoV‐2 Variants of Interest

Point-of-care isothermal nucleic acid amplification tests: progress and bottlenecks for extraction-free sample collection and preparation

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